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New Somatic Mutation in the PIG-A Gene Emerges at Relapse of Paroxysmal Nocturnal Hemoglobinuria by Khédoudja Nafa, Monica Bessler, H. Joachim Deeg, and Lucio Luzzatto Blood Volume 92(9):3422-3427 November 1, 1998 ©1998 by American Society of Hematology Long history of PNH in patient MSK13. Khédoudja Nafa et al. Blood 1998;92:3422-3427 ©1998 by American Society of Hematology Flow cytometry analysis with anti-CD59 of PMN from a normal control (broken line) and PNH patient MSK13 (full line). Khédoudja Nafa et al. Blood 1998;92:3422-3427 ©1998 by American Society of Hematology Analysis of exon 6 of the PIG-A gene before BMT and after relapse. Khédoudja Nafa et al. Blood 1998;92:3422-3427 ©1998 by American Society of Hematology Insertion duplication in exon 6 of the PIG-A gene in patient MSK13. Khédoudja Nafa et al. Blood 1998;92:3422-3427 ©1998 by American Society of Hematology Khédoudja Nafa et al. Blood 1998;92:3422-3427 ©1998 by American Society of Hematology Khédoudja Nafa et al. Blood 1998;92:3422-3427 ©1998 by American Society of Hematology Point mutations in exon 2 of the PIG-A gene in patient MSK13 before BMT. (A) Exon 2 of the PIGA gene was first amplified by external primers -I1 and I2, and then reamplified with the internal primers (see Table 1). Khédoudja Nafa et al. Blood 1998;92:3422-3427 ©1998 by American Society of Hematology