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The different process of class switching and somatic hypermutation; a novel analysis by CD27− naive B cells by Haruo Nagumo, Kazunaga Agematsu, Norimoto Kobayashi, Koji Shinozaki, Sho Hokibara, Hisashi Nagase, Masaya Takamoto, Kozo Yasui, Kazuo Sugane, and Atsushi Komiyama Blood Volume 99(2):567-575 January 15, 2002 ©2002 by American Society of Hematology Preparation of adult CD27− naive and CB B cells.Highly purified adult CD27− B cells were separated by means of flow cytometry, and CB B cells were separated with the aid of CD19coated immunomagnetic beads. Haruo Nagumo et al. Blood 2002;99:567-575 ©2002 by American Society of Hematology TGF-β does not induce IgA production from adult CD27− and CB B cells.Highly purified adult CD27+, CD27−, and CB B cells were cultured with TGF-β (0.1, 1, and 10 ng/mL) in the presence of SAC (0.01%) + IL-2 (50 U/mL) + IL-10 (50 ng/mL) + anti-CD40 mAb (1 μg/... Haruo Nagumo et al. Blood 2002;99:567-575 ©2002 by American Society of Hematology Induction of mature γ1 and γ2 transcripts from naive B cells.Highly purified adult CD20+CD27+, CD20+CD27−, or CB B cells at the cell numbers of 0.5 × 106 cells were cultured with medium alone (lanes 1, 4, 7), 0.01% SAC, 50 U/mL IL-2, 50 ng/mL IL-10, and 1 μ... Haruo Nagumo et al. Blood 2002;99:567-575 ©2002 by American Society of Hematology Induction of AID from naive B cells.Highly purified adult CD20+CD27+, CD20+CD27−, or CB B cells at the cell numbers of 0.5 × 106 cells were cultured with medium alone (lanes 1, 4, 7), 0.01% SAC, 50 U/mL IL-2, 50 ng/mL IL-10, and 1 μg/mL anti-CD40 cross-link... Haruo Nagumo et al. Blood 2002;99:567-575 ©2002 by American Society of Hematology Induction of CD27 and analysis of somatic hypermutations in CD27+ B cells derived from naive B cells.(A) Highly purified CB B cells were stimulated with medium alone, 1 μg/mL anti-CD40 + CD32T, or 0.01% SAC + 50 U/mL IL-2. Haruo Nagumo et al. Blood 2002;99:567-575 ©2002 by American Society of Hematology Induction of plasma cells from CD27− naive B cells.Highly purified adult CD27− B cells were cultured with SAC (0.01%) + IL-2 (50 U/mL), or IL-10 (50 ng/mL) + anti-CD40 mAb (1 μg/mL), SAC + IL-2 + IL-10 + anti-CD40 mAb in conjunction with cross-linking by CD... Haruo Nagumo et al. Blood 2002;99:567-575 ©2002 by American Society of Hematology The characteristics and amino acid sequences of VH5 genes in plasma cells generated from naive B cells.(A) Highly purified CB B cells were cultured with SAC (0.01%), IL-2 (50 U/mL), IL-10 (50 ng/mL), and anti-CD40 mAb (1 μg/mL) with CD32T for 8 days. Haruo Nagumo et al. Blood 2002;99:567-575 ©2002 by American Society of Hematology The different process of class switching and somatic hypermutation and proposed generation of plasma cells in humans.After B-cell precursors succeed in generating functional antigen receptors in bone marrow, they are released into the B-cell pool as naive B... Haruo Nagumo et al. Blood 2002;99:567-575 ©2002 by American Society of Hematology
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