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GATA3 up-regulation associated with surface expression of CD294/CRTH2: a unique feature of human Th cells by Umberto De Fanis, Francesca Mori, Rebecca J. Kurnat, Won Kyung Lee, Maria Bova, N. Franklin Adkinson, and Vincenzo Casolaro Blood Volume 109(10):4343-4350 May 15, 2007 ©2007 by American Society of Hematology In vitro polarization of human Th naive precursors. Umberto De Fanis et al. Blood 2007;109:4343-4350 ©2007 by American Society of Hematology The GATA-3 gene is expressed at similar levels in human Th1- and Th2-skewed cultures. Umberto De Fanis et al. Blood 2007;109:4343-4350 ©2007 by American Society of Hematology The occurrence of CRTH2+ and CCR5+ cells in human Th-cell–polarizing cultures. Umberto De Fanis et al. Blood 2007;109:4343-4350 ©2007 by American Society of Hematology CRTH2+ Th cells exhibit an “enhanced” Th2 phenotype. Umberto De Fanis et al. Blood 2007;109:4343-4350 ©2007 by American Society of Hematology Quantitative analysis of gene expression in Th2-skewed cells sorted by CRTH2 or IL-4 expression. Umberto De Fanis et al. Blood 2007;109:4343-4350 ©2007 by American Society of Hematology Ex vivo characterization of circulating CD4+CRTH2+ T cells. Umberto De Fanis et al. Blood 2007;109:4343-4350 ©2007 by American Society of Hematology