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A Truncated Isoform of the Human β Chain Common to the Receptors for Granulocyte-Macrophage ColonyStimulating Factor, Interleukin-3 (IL-3), and IL-5 With Increased mRNA Expression in Some Patients With Acute Leukemia by Rosemary E. Gale, Robin W. Freeburn, Asim Khwaja, Rajesh Chopra, and David C. Linch Blood Volume 91(1):54-63 January 1, 1998 ©1998 by American Society of Hematology RT-PCR analysis of the GMRβC chain between nucleotides 1281 and 1620 of the published sequence17 using RNA from hematopoietic cell lines (TF-1 and U937) and leukemic blasts of 2 patients with AML (Pt). Rosemary E. Gale et al. Blood 1998;91:54-63 ©1998 by American Society of Hematology (A) Protein structure of the GMRβC chain compared with predicted structure of the βIT chain formed by splicing out the 104-bp exon in the membrane-proximal region. Rosemary E. Gale et al. Blood 1998;91:54-63 ©1998 by American Society of Hematology RNAse protection analysis of the GMRβ chain using total RNA from hematopoietic cell lines (TF1), primary myeloid cells (purified neutrophils, CD34+ cells, and mononuclear cells from normal bone marrow), and leukemic blasts from patients with AML. The full-... Rosemary E. Gale et al. Blood 1998;91:54-63 ©1998 by American Society of Hematology Relative expression of βIT mRNA as detected by RNAse protection assays (•) or semiquantitative RT-PCR (○) on total RNA from hematopoietic cell lines, primary myeloid cells, and leukemic blasts of patients with ALL or AML of varying FAB types (M0 to M6). Rosemary E. Gale et al. Blood 1998;91:54-63 ©1998 by American Society of Hematology Scatchard analysis of 125I-GM-CSF binding to COS-7 cells transiently transfected with either GMRα (○) or GMRα + βIT (•). Rosemary E. Gale et al. Blood 1998;91:54-63 ©1998 by American Society of Hematology JAK2 association with the βIT chain and subsequent phosphorylation by stimulation with GMCSF. Rosemary E. Gale et al. Blood 1998;91:54-63 ©1998 by American Society of Hematology Protein expression of the βIT chain. Rosemary E. Gale et al. Blood 1998;91:54-63 ©1998 by American Society of Hematology [3H]thymidine uptake assay of CTLL-2 clones transfected with vector (○), GMRα (•), GMRα + βC (αβC), or GMRα + βIT(αβIT) and stimulated with increasing concentrations of GM-CSF. Rosemary E. Gale et al. Blood 1998;91:54-63 ©1998 by American Society of Hematology
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