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Efficient In Vivo Marking of Primary CD4+ T Lymphocytes in Nonhuman Primates Using a Gibbon Ape Leukemia VirusDerived Retroviral Vector by Bruce A. Bunnell, Mark Metzger, Ellen Byrne, Richard A. Morgan, and Robert E. Donahue Blood Volume 89(6):1987-1995 March 15, 1997 ©1997 by American Society of Hematology Map of the retroviral vector GC-(anti–Tat/Rev) SNDC. The vector contains an antisense Tat/Rev gene that encompasses the entire Rev sequence and the second exon of the Tat gene. Bruce A. Bunnell et al. Blood 1997;89:1987-1995 ©1997 by American Society of Hematology Detection and persistence of transduced CD4+ lymphocytes in the PB. Following each reinfusion of transduced lymphocytes, peripheral blood samples were isolated at regular intervals and used to isolate PBMC. Semiquantitative PCR for the NeoR gene was perform... Bruce A. Bunnell et al. Blood 1997;89:1987-1995 ©1997 by American Society of Hematology (A) Detection of the transduced lymphocytes in vivo. Bruce A. Bunnell et al. Blood 1997;89:1987-1995 ©1997 by American Society of Hematology Expression of the Tat/Rev antisense gene. Bruce A. Bunnell et al. Blood 1997;89:1987-1995 ©1997 by American Society of Hematology
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