Download Enzymes, Essential for Life!

Flora Manufacturing
November 21, 2005
Contents
The Digestive System
Enzyme Units
TNO Research
Flora Digestive Products
Enzymes in Digestion
Mouth
Carbohydrates
-amylase
Stomach
Proteins
Pepsin
Small
Intestine
Carbohydrates
amylase, sucrase,
lactase, maltase
trypsin, chymotrypsin
carboxypeptidase, etc.
lipase
Proteins
Fats
Endogenous to the human body
Digestive System
Supplementary digestive
enzymes are most effective when
they work in the stomach and
small intestine, and so must work
over a broad pH range.
When digestion is more rapid and
thorough, ingested material
passes more quickly through the
digestive tract. This addresses
problems which arise when food
sits in the stomach too long
causing excess acid and
indigestion.
pH 1.0 - 3.0
pH 6.0 - 8.0
Most Common Problems Associated with
Improper Digestion
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Constipation, gas, bloating, heartburn
Intolerance and allergies
Reduced function of the immune system
Lack of energy
What does a comprehensive digestive
enzyme formula look like?
Lipases; break down fat
as is found in butter,
cheese, and meat.
Proteases;break down
protein as is found in
meats, nuts, and
cheese.
Carbohydrases - break
down starches,
polysaccharides,
sugars, and fiber as are
found in fruits,
vegetables, grains, and
snack foods.
How Do You Recognize a Good
Formula?
•Activity Units and
not Weight
•Broad Range of
Activity
•Acid Stability
•Fungal Enzymes
Anecdotal Evidence:
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I feel better after taking enzymes
I have reduced gas, bloating,
constipation…
I eat less if I take enzymes
I feel I am absorbing more from my
food
Only qualitative data, no quantitative
results
Do Fungal Enzymes increase
the digestibility and
bioaccessibility of
proteins and carbohydrates?
TNO: Background
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TNO Nutrition and Food Research
Independent research organization
in the Netherlands
Link between fundamental research
and its application to food and drugs
NEC – TNO collaboration
TNO: What is TIM?
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in vitro Dynamic gastrointestinal
model
Simulate stomach, small intestine
and large intestine conditions
Sampling during digestion
Specific protocol development and
validation
STOMACH
DUODENUM
JEJUNUM
ILEUM
Study Protocol
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Aim: To determine the efficacy of
exogenous NEC enzymes on the
digestibility of proteins and carbs
Standard FDA-type test meal
Proteins, carbohydrates and fat
Mouth, stomach and small intestine
conditions
Protocol: cont’d
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Meal mixed with artificial saliva and
water
Stomach secretions: acid and pepsin
Duodenum: bicarbonate and
pancreatin
Small intestine
Protocol: cont’d
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Gastric emptying and intestinal
passage mimicked for humans
Temperature, peristalsis, pH, etc…
controlled by computer
Sampling spots: Jejunum and Ileum
Small intestine: Semi-permeable
membrane
Conditions Tested
1.
2.
3.
4.
Perfect digestion
Perfect digestion with NEC
enzymes
Impaired digestion (30% of
pancreatin)
Impaired digestion with NEC
enzymes
Most of us fall in between these
two extreme conditions
Condition 1: Perfect Digestion
Jejunal Glucose Bioaccessibility
Perfect Digestion
Perfect Digestion with NEC Enzymes
Glucose Absorbed g/L
2
1.8
1.6
1.4
1.2
1
0.8
0.6
0.4
0.2
0
0-1 Hours
1-2 Hours
2-3 Hours
3-5 Hours
Ileal Glucose Bioaccessibility
Perfect Digestion
Perfect Digestion with NEC Enzymes
1.6
Glucose Absorbed g/L
1.4
1.2
1
0.8
0.6
0.4
0.2
0
0-1 Hours
1-2 Hours
2-3 Hours
3-5 Hours
Total Free Glucose Bioaccessibility
Small Intestine (Perfect Digestion)
2
1.5
1
0.5
0
Jejunum
Control with NEC Enzymes
2.5
Control
3
Control with NEC Enzymes
3.5
Control
Glucose Absorbed g/L
4
Ileum
Total Free Nitrogen Bioaccessibility
Small Intestine (Perfect Digestion)
3.5
1
0.5
Control with NEC Enzymes
1.5
Control
2
Control with NEC Enzymes
2.5
Control
Nitrogen mmol/L
3
0
Jejunum
Ileum
Condition 2: Impaired Digestion
Jejunal Glucose Bioaccessibility
Impaired Digestion
Impaired Digestion with NEC Enzymes
Glucose Absorbed g/L
2
1.8
1.6
1.4
1.2
1
0.8
0.6
0.4
0.2
0
0-1 Hours
1-2 Hours
2-3 Hours
3-5 Hours
Ileal Glucose Bioaccessibility
Impaired Digestion
Impaired Digestion with NEC Enzymes
Glucose Absorbed g/L
0.9
0.8
0.7
0.6
0.5
0.4
0.3
0.2
0.1
0
0-1 Hours
1-2 Hours
2-3 Hours
3-5 Hours
Total Free Glucose Bioaccessibility
Small Intestine (Impaired Digestion)
3
2.5
2
1.5
1
0.5
0
Jejunum
Control with
NEC Enzymes
3.5
Control
4
Control with NEC Enzymes
4.5
Control
Glucose Absorbed g/L
5
Ileum
Total Free Nitrogen Bioaccessibility
Small Intestine (Impaired Digestion)
1
0.5
0
Jejunum
Control with
NEC Enzymes
1.5
Control
2
Control with NEC Enzymes
2.5
Control
Nitrogen mmol/L
3
Ileum
Importance of study
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First quantitative evidence supporting the
use of supplemental digestive enzymes
NEC fungal enzymes do not need enteric
coating
NEC digestive enzymes improve the
digestibility and bioaccessibility of proteins
and carbs
Proves efficacy of NEC digestive enzymes
Flora: Digestive Enzyme Products
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Teenager’s Blend Enzyme
Adult’s Blend Enzyme
Advanced Adult Enzyme
Anti-Inflammatory Enzyme
Teenager’s Enzyme Blend
Amylase……………………………..5000 DU
Alpha-G……………………………...100 GalU
Protease 4.5…………………………5000 HUT
Glucoamylase………………………10 AGU
Lipase AN……………………………250 FIP
Malt Diastase………………………..250 DP
Protease 6.0…………………………1500 HUT
Invertase……………………………..250 SU
Cellulase……………………………..100 CU
Protease (A. niger)………………….399.3 BLGU
Protease (A. oryzae)………………..230.5 BLGU
Lactase……………………………….1000 ALU
Teenager’s Enzyme Blend
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Aid digestion of proteins, carbs,
sugars, fats and dairy
Maximize absorption of nutrients
Geared towards teens with
active lifestyle
Addresses intolerances and
other discomfort caused by
certain foods
Adult’s Enzyme Blend
Amylase…………………………………....5000 DU
Protease 4.5……………………………….10000 HUT
Lipase AN………………………………….1000 FIP
Bromelain………………………………….500000 FCC PU
Protease 6.0………………………………8000 HUT
Protease 3.0………………………………15 SAPU
Alpha-G……………………………………125 GALU
Glucoamylase……………………………..8 AGU
Peptidase…………………………………..2000 HUT
Malt Diastase………………………………300 DP
Invertase……………………………………150 SU
Cellulase……………………………………275 CU
Hemicellulase……………………………...758.8 HCU
Phytase……………………………………..1.9 FTU
Adult’s Enzyme Blend
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Support digestion for adults on
restrictive diets
Efficiently digest processed and
nutrient dense meals
Wide pH of proteolytic activity
Help alleviate gas, bloating,
constipation, heartburn
Advanced Adult’s Enzyme
Amylase…………………………………....5000 DU
Protease 4.5……………………………….14000 HUT
Protease 6.0……………………………….10000 HUT
Alpha-G………………………………………….175 GALU
Bromelain………………………………….500000 FCC PU
Protease 3.0………………………………15 SAPU
Glucoamylase……………………………..8 AGU
Peptidase…………………………………..2000 HUT
Lipase AN……………………………………….75 FIP
Lipase RO……………………………………….250 FIP
Malt Diastase………………………………300 DP
Invertase……………………………………150 SU
Protease (A. niger)……………………………..399.3 BLGU
Protease (A. oryzae)…………………………...230.5 BLGU
Lactase…………………………………………..1000 ALU
Cellulase……………………………………275 CU
Hemicellulase……………………………...758.8 HCU
Phytase……………………………………..1.9 FTU
Advanced Adult’s Enzyme
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Full spectrum digestive enzyme
formula
Help maximize absorption of
nutrients
Alleviate symptoms of gas,
bloating, constipation, heartburn
High proteolytic activity
Anti-inflammatory Enzyme
Protease AM………………………………….25 AP
Bromelain……………………………………..750000 FCC PU
Protease 6.0………………………………….10000 HUT
Papain………………………………………...500000 FCC PU
Peptidase……………………………………..2500 HUT
Protease (A. melleus)……………………….14950 U
Protease (A. oryzae)………………………...50 U
Anti-inflammatory Enzyme
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Proteolytic enzymes designed to
mediate inflammatory response
Joints and soft tissue as well as
intestinal inflammation
Specifically breakdown certain
cytokines
Fibrinolytic activity