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Figure legends to Supplementary data
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Table S1. Affiliation and accession number to OTUs from nifH gene clone libraries, 16S
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rRNA and nifH gene DGGE bands.
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Figure S1. Nitrate concentration along the light period for temperatures of 52 and 47 ºC
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during year 2012 at Porcelana hot spring.
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Figure S2. Phylogenetic affiliation of sequences retrieved using cyanobacterial specific 16S
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rRNA genes as targets combined with DGGE analyses of microbial mat associated DNA
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samples from Porcelana hot spring (Chile). The phylogram was constructed using the
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maximum likelihood distance method. Sequences obtained are given in bold, and each DGGE
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phylotype is designated by their accession number. The numberings are the same as those
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given in the 16S rRNA-DGGE profiles in Fig. S3a. The numbers given at the nodes represent
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bootstrap values of 10.000 replicates.
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Figure S3. DGGE fingerprint images showing microbial mat associated DNA samples along
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the temperature gradient and the four years investigated at Porcelana hot spring (Chile). For
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identity results (phylogenetic reconstructions), see Supplementary data, Figure S2 and S4. a)
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Image of the 16S rRNA gene analysis with cyanobacterial specific 16S rRNA primers,
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indicating the sequenced bands CYA1-CYA5. b) Image of the nifH gene analysis with
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cyanobacterial specific nifH primers, indicating the sequenced bands CN1-CN3.
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Abbreviations used in a): CYA1, CYA2, CYA3, CYA4 = Oscillatoriales and CYA5 =
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Stigonematales. Abbreviations used in b) CN1, CN2 y CN3= Stigonematales.
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Figure S4. Phylogenetic affiliation of sequences retrieved using universal and cyanobacterial
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specific nifH genes as targets combined with clone libraries (OTUs) and DGGE (phylotypes)
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analyses of microbial mat associated DNA samples from Porcelana hot spring (Chile). The
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phylogram was constructed using the maximum likelihood distance method. Sequences
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obtained are given in bold, and each OTU and DGGE phylotype is designated by their
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accession number. The numberings are the same as those given in the Supplementary data,
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Table S1 and nifH-DGGE profiles in the Fig. S3b. The numbers given at the nodes represent
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bootstrap values of 10.000 replicates.
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Figure S5. Canonical Correspondence Analysis (CCA) for the total environmental and
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biological variables at the four years of study (2009, 2011-2013).
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Figure S6. Redundancy analysis (RDA) for total environmental and biological variables
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obtained at the four years of study (2009, 2011-2013).
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