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Consulta: subjectFacets:"Tetraploid" Registros recuperados: 6 Data/hora: 09/06/2017 09:53:38 A new variety of Bacopa monnieri obtained by in vitro polyploidization Provedor de dados: 69 Autores: Salvio Escandón,Alejandro; Hagiwara,Juan Carlos; Alderete,Liliana Marisol. The commercial value of Bacopa monnieri, a widespread herbaceous plant in Argentina, can be substantially improved increasing its flower size by chromosome doubling with colchicine. MS supplemented with 0.25 mg/L of 6-benzylaminopurine proved to be an appropriate medium for the in vitro multiplication of nodal segments of B. monnieri. Polyploidization was achieved submerging nodal segments during 24 or 48 hrs in colchicine solution 0.001 and 0.01% P/V, in 1% DMSO. Segments submerged in water and in DMSO 1% aqueous solution were used as controls. DNA contents from recovered plants was measured and characterized and their phenotype described. Two tetraploid plants originated from independent events were detected. These plants showed significant differences... Tipo: Journal article Palavras-chave: Colchicine; Flow cytometry; Ornamental plants; Scrophulariaceae; Tetraploid. Ano: 2006 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000300002 Changes in motility, ATP content, morphology and fertilisation capacity during the movement phase of tetraploid Pacific oyster (Crassostrea gigas) sperm Provedor de dados: 5 Autores: Suquet, Marc; Labbe, C.; Brizard, Raphael; Donval, Anne; Le Coz, Jean-rene; Quere, Claudie; Haffray, P. Changes in sperm features during the movement phase are especially interesting to study in external fertilization species whose sperm duration movement is long because this implies a significant adaptation of moving cells to the external medium. This study describes the changes in tetraploid Pacific oyster sperm characteristics in relation to time post activation. Sperm individually collected on three tetraploid males were activated in seawater. Their features were analysed over a 24 h period and compared to a sperm pool collected on three diploid males as a reference. The percentage of motile spermatozoa, the intracellular ATP content, and the fine structure of spermatozoa were studied in relation to time post activation. Furthermore, the fertilisation... Tipo: Text Palavras-chave: Crassostrea gigas; Sperm; Tetraploid; Motility; ATP; Ultrastructure. Ano: 2010 URL: http://archimer.ifremer.fr/doc/00006/11756/8639.pdf Chromosome loss in bi-parental progenies of tetraploid Pacific oyster Crassostrea gigas Provedor de dados: 5 Autores: Mccombie, Helen; Lapegue, Sylvie; Cornette, Florence; Ledu, Christophe; Boudry, Pierre. Alterations of chromosome number have been observed in the somatic tissue of Crassostrea gigas diploids and artificial polyploids. Tetraploid oysters with abnormal chromosome numbers in some or all of their tissue are considered undesirable as parents either for triploids (produced via a cross with diploids and of aquacultural interest) or for tetraploid breeding. Aneuploid tetraploid oysters may confer the tendency to lose chromosomes and to revert to lower ploidy levels to their offspring. More directly, their offspring could have a lower ploidy because of potential links between somatic and gametic chromosome loss. The present study evaluated the phenomenon in six bi-parental tetraploid families bred from parents of differing somatic ploidy quality. The... Tipo: Text Palavras-chave: Crassostrea gigas; Mitosis; Chromosome; Cytogenetics; Aneuploid; Polyptoid; Tetraploid; Oyster. Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/publication-633.pdf Chromosome loss in tetraploid families of the Pacific oyster Crassostrea gigas Provedor de dados: 5 Autores: Mccombie, Helen; Lapegue, Sylvie; Cornette, Florence; Ledu, Christophe; Boudry, Pierre. Alterations of chromosome number have been observed in the somatic tissue of Crassostrea gigas diploids and artificial polyploids bred in this species. Tetraploid animals with lower chromosome counts in some or all of their somatic tissue are considered undesirable as parents either for commercial triploids (produced via a cross with diploids) or for tetraploid breeding because (1) they may confer the tendency to lose chromosomes and revert to lower ploidy levels to their offspring or (2) their offspring could have a lower ploidy directly because of potential links between somatic and gametic chromosome loss. The present study evaluated the phenomenon in bi-parental tetraploid families bred from parents of differing ploidy quality, including a... Tipo: Text Palavras-chave: Tetraploid; Chromosome; Genetic; Crassostrea gigas; Oyster. Ano: 2003 URL: http://archimer.ifremer.fr/doc/2003/acte-3435.pdf New varieties of Magnolia biondii and Magnolia cylindrica (Magnoliaceae) in China Provedor de dados: 16 Autores: Wang, Y.L.; Ejder, E.; Yang, J.F.; Kang, Y.X.; Ye, W.; Zhang, S.Z. Magnolia biondii var. purpurascens var. nov. and Magnolia cylindrica var. purpurascens var. nov. (Magnoliaceae) are described as two new varieties endemic to China. Magnolia biondii var. purpurascens is mainly restricted to forests of Shaanxi and SE Gansu provinces. It differs from the typical variety, M. biondii var. biondii, in the dense indumentum of white hairs on twigs and the cup-shaped purplish red flowers. Magnolia cylindrica var. purpurascens occurs in secondary forests of Jingning and Songyang counties of southern Zhejiang province. It differs from the typical variety, M. cylindrica var. cylindrica, in the cup-shaped flowers of purplish red colour. The two new varieties are distinct in their distributions in both cases but share the chromosome... Tipo: Article / Letter to the editor Palavras-chave: China; Magnolia; Magnolia biondii; Magnolia cylindrica; Magnoliaceae; Tetraploid; Variety. Ano: 2013 URL: http://www.repository.naturalis.nl/record/525455 Wild ornamental germplasm exploration and domestication based on biotechnological approaches: In vitro colchicine treatment to obtain a new cultivar of Scoparia montevidiensis Provedor de dados: 69 Autores: Escandón,Alejandro S; Miyajima,Ikuo; Alderete,Marisol; Hagiwara,Juan Carlos; Facciuto,Gabriela; Mata,Diego; Soto,Silvina M. The genus Scoparia is native from Argentina. The diversity of colours and shape of their flowers bestows this genus a very interesting ornamental potential. The purpose of the present study is to explore the Scoparia species germplasm by means of in vitro polyploidization in order to improve their ornamental qualities. Accessions of S. montevidiensis var. montevidiensis, S. montevidiensis var. glandulifera, S. nudicaulis, S. hasleriana and S. dulcis were collected and maintained under greenhouse conditions. The Murashige-Skoog medium, supplemented with 0.25 mg/L BAP was used for the nodal segments multiplication of the five Scoparia species. Except for S. hasleriana, the multiplication rate of the other species ranged between 10 and 12 shoots per explant.... Tipo: Journal article Palavras-chave: Chimera; Flow cytometry; Micropropagation; Ornamental plants; Tetraploid. Ano: 2005 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582005000200010