Download Consulta: creatorFacets:"Ferreres, F." Registros recuperados: 32

Consulta: creatorFacets:"Ferreres, F."
Registros recuperados: 32
Data/hora: 09/06/2017 01:10:23
Analysis and quantification of flavonoidic compounds from Portuguese olive (Olea europeae L.) leaf
cultivars
Provedor de dados: 188
Autores: Meirinhos, J.; Silva, B.M.; Valentão, P.; Seabra, R.M.; Pereira, J.A.; Dias, A.; Andrade, P.B.; Ferreres, F.
Twenty three samples of 18 Portuguese olive leaf cultivars were analysed by a reversed-phase HPLC/DAD procedure and eight
flavonoidic compounds were identified and quantified (luteolin 7,40-O-diglucoside, luteolin 7-O-glucoside, rutin, apigenin
7-O-rutinoside, luteolin 40-O-glucoside, luteolin, apigenin and diosmetin). Luteolin 7,40-O-diglucoside and luteolin
40-O-glucoside were identified by HPLC/DAD/MS/MS – ESI. The studied olive leaf samples showed a common phenolic pattern,
in which luteolin 40-O-glucoside was almost always the major compound
Tipo: Article
Palavras-chave: Olea europaea L.; Olive leaves; Olive cultivars; HPLC-DAD/MS/MS/ESI; Flavonoids.
Ano: 2005
URL: http://hdl.handle.net/10198/728
Antioxidative properties of tronchuda cabbage (Brassica oleraceae var. costata) external leaves
against DPPH, superoxide radical, hydroxyl radical and hypochlorous acid
Provedor de dados: 188
Autores: Vrchovska, V.; Sousa, C.; Valentão, P.; Ferreres, F.; Pereira, J.A.; Seabra, R.M.; Andrade, P.B.
The ability of the aqueous extract of tronchuda cabbage (Brassica oleracea L. var. costata DC) external leaves to act as a
scavenger of DPPH- and reactive oxygen species (superoxide radical, hydroxyl radical and hypochlorous acid) was investigated.
A phytochemical study was also undertaken, and thirteen phenolic compounds and five organic acids were identified and
quantified. Tronchuda cabbage extracts exhibited antioxidant capacity in a concentration-dependent manner in all assays, although
some pro-oxidant effect was also noticed. The samples with higher phenolic and organic acid contents displayed the major
antioxidant potentials.
Tipo: Article
Palavras-chave: Tronchuda cabbage; Brassica oleracea L. var. costata DC; DPPH; Superoxide radical; Hydroxyl radical;
Hypochlorous acid; Phenolics; Organic acids.
Ano: 2006
URL: http://hdl.handle.net/10198/744
Chemical assessment and in vitro antioxidant capacity of Ficus carica latex
Provedor de dados: 188
Autores: Oliveira, A.P.; Silva, L.R.; Ferreres, F.; Pinho, P. Guedes de; Valentão, P.; Silva, B.M.; Pereira, J.A.; Andrade, P.B.
Ficus species possess latex-like material within their vasculatures, affording protection and selfhealing from physical attacks. In
this work, metabolite profiling was performed on Ficus carica latex. Volatiles profile was determined by HS-SPME/GC-IT-MS,
with 34 compounds being identified, distributed by distinct chemical classes: 5 aldehydes, 7 alcohols, 1 ketone, 9 monoterpenes, 9
sesquiterpenes and 3 other compounds. Sesquiterpenes constituted the most abundant class in latex (ca. 91% of total identified
compounds). Organic acids composition was also characterized, by HPLC-UV, and oxalic, citric, malic, quinic, shikimic and
fumaric acids were determined. Malic and shikimic acids were present in higher amounts (ca. 26%, each). The antioxidant
potential...
Tipo: Article
Palavras-chave: Ficus carica latex; Volatiles; Organic acids; Antioxidant potential; Acetylcholinesterase inhibition.
Ano: 2010
URL: http://hdl.handle.net/10198/3116
Chemical composition and antioxidant activity of tronchuda cabbage internal leaves
Provedor de dados: 188
Autores: Ferreres, F.; Sousa, C.; Vrchovska, V.; Valentão, P.; Pereira, J.A.; Seabra, R.M.; Andrade, P.B.
A phytochemical study was undertaken on the internal leaves of tronchuda cabbage (Brassica oleracea L. var. costata DC).
Seventeen phenolic compounds were characterized and quantified by reversed-phase HPLCDAD-ESI-MSn and HPLC/DAD,
respectively: quercetin 3-O-sophoroside-7-O-glucoside, 3-p-coumaroylquinic acid, kaempferol 3-O-sophoroside-7-O-glucoside,
kaempferol
3-O-(caffeoyl)-sophoroside-7-O-glucoside,
3-O-(sinapoyl)-sophoroside-7-Oglucoside,
kaempferol
sinapoyl
glucoside
acid,
kaempferol
3-O-(feruloyl)-sophoroside-7-Oglucoside,
kaempferol
3-O-(p-coumaroyl)-sophoroside- 7-O-glucoside, 4-p-coumaroylquinic acid, sinapic acid, kaempferol 3-O-sophoroside, 3 isomeric
forms of 1,2- disinapoylgentiobiose, 1-sinapoyl-2-feruloylgentiobiose,...
Tipo: Article
Palavras-chave: Tronchuda cabbage internal leaves; Brassica oleracea L. var. costata DC; Phenolics; Organic acids; Antioxidant
capacity.
Ano: 2006
URL: http://hdl.handle.net/10198/738
Composição química e actividade antioxidante das folhas internas da couve tronchuda
Provedor de dados: 188
Autores: Pereira, J.A.; Sousa, C.; Ferreres, F.; Valentão, P.; Seabra, R.M.; Andrade, P.B.
A couve tronchuda (Brassica oleracea L. var. costata DC) é uma das couves mais consumidas pelos portugueses, especialmente
durante o Inverno, apresentando as folhas (internas e externas) diferenças organolépticas marcadas. No presente trabalho
procedeu-se à caracterização química das folhas internas de couve tronchuda, através da identificação e quantificação dos
compostos fenólicos e dos ácidos orgânicos que as compõem e avaliou-se o seu potencial antioxidante. Foram identificados e
quantificados,
por
HPLC-DAD-ESI-MS
3-O-soforósido-7-O-glucósido,
ácido
e
HPLC/DAD,
3-p-cumaroilquínico,
respectivamente,
campferol
17
compostos
fenólicos:
3-O-soforósido-7-O-glucósido,
quercetina
campferol
3-O-(cafeoil)-soforósido-7-O-glucósido, ácido sinapoilglucósido,...
Tipo: ConferenceObject
Palavras-chave: Couve tronchuda; Compostos fenólicos; Ácidos orgânicos; Actividade antioxidante.
Ano: 2007
URL: http://hdl.handle.net/10198/6040
Flavonóides em folhas de cultivares portuguesas de oliveira.
Provedor de dados: 188
Autores: Meirinhos, J.; Silva, B.M.; Valentão, P.; Seabra, R.M.; Pereira, J.A.; Dias, A.; Andrade, P.B.; Ferreres, F.
Tipo: ConferenceObject
Ano: 2005
URL: http://hdl.handle.net/10198/5561
Free water-soluble phenolics profiling in barley (Hordeum vulgare L.)
Provedor de dados: 188
Autores: Ferreres, F.; Kršková, Z.; Gonçalves, Rui; Valentão, P.; Pereira, J.A.; Dušek, J.; Martin, J.; Andrade, P.B.
The phenolic profile of barley (Hordeum vulgare L.) leaves, seeds, awns, and stems, collected in two different locations from
Portugal, was determined by a high-performance liquid chromatography/ diode array detector (HPLC/DAD). A total of 28
compounds were identified and quantified, which included 4 phenolic acids, 6 C-glycosylflavones, and 18 O-glycosyl-C-glycosyl
flavones, with some of them acylated. Distinct profiles were noticed among the analyzed materials. The greatest diversity of
compounds was found in barley leaves (26 flavonoids and 2 phenolic acid derivatives), which also exhibited the highest
concentration of phenolics. Isoorientin-7-O-glucoside (lutonarin) was the major compound in leaves, while, in general, the pair
isovitexin-7-O-rutinoside...
Tipo: Article
Palavras-chave: Barley; Hordeum vulgare L.; Phenolic compounds; Vegetal materials; HPLC/DAD.
Ano: 2009
URL: http://hdl.handle.net/10198/3047
Hazel (Corylus avellana L.) leaves as source of antimicrobial and antioxidative compounds
Provedor de dados: 188
Autores: Oliveira, I.; Sousa, A.; Valentão, P.; Andrade, P.B.; Ferreira, Isabel C.F.R.; Ferreres, F.; Bento, Albino; Seabra, R.M.;
Estevinho, Leticia M.; Pereira, J.A.
Aqueous extracts of leaves of different hazel (Corylus avellana L.) cultivars (Cv. M. Bollwiller, Fertille de Coutard and Daviana),
were analysed by reversed-phase HPLC/DAD for the definition of their phenolic composition. Antioxidant potential was assessed
by the reducing power assay, and the scavenging effect on DPPH (2,2-diphenyl-1-picrylhydrazyl) radicals and b-carotene
linoleate model system. Their antimicrobial capacity was also tested against Gram positive (Bacillus cereus, Bacillus subtilis,
Staphylococcus aureus) and Gram negative bacteria (Pseudomonas aeruginosa, Escherichia coli, Klebsiella pneumoniae) and
fungi (Candida albicans, Cryptococcus neoformans). Eight phenolic compounds were identified: 3-, 4- and 5-caffeoylquinic
acids,...
Tipo: Article
Palavras-chave: Hazel leaves; Phenolics; Antioxidant potential; Antimicrobial activity.
Ano: 2007
URL: http://hdl.handle.net/10198/753
HPLC-DAD-MS/MS-ESI screening of phenolic compounds in Pieris brassicae L. reared on Brassica
rapa var. rapa L..
Provedor de dados: 188
Autores: Ferreres, F.; Valentão, P.; Pereira, J.A.; Bento, Albino; Noites, A.; Seabra, R.M.; Andrade, P.B.
The phenolic profiles of cabbage white butterfly (Pieris brassicae L.; Lepidoptera: Pieridae) at different development stages
(larvae, exuviae, and butterfly), its excrements, and its host plant Brassica rapa var. rapa L. were determined by high performance
liquid
chromatography-
diode-array
detector-mass
spectrometry/mass
spectrometry-electrospray
ionization
(HPLC-DAD-MS/MS-ESI). Twenty-five acylated and nonacylated flavonoid glycosides and ferulic and sinapic acids were
identified in host plant, from which only 12 compounds were found in the excrements. In addition, the excrements showed the
presence of sulfate flavonoids and other flavonoid glycosides that were not detected in the leaves. In the larvae kept without food
for 12 h, only 3 compounds common...
Tipo: Article
Palavras-chave: Pieris brassicae L; Brassica rapa var. rapa L.; Turnip; Phenolic compounds; HPLC-DAD/MS/MS/ESI.
Ano: 2008
URL: http://hdl.handle.net/10198/775
In vitro cultures of Brassica oleracea L. var. costata DC: potential plant bioreactor for antioxidant
phenolic compounds.
Provedor de dados: 188
Autores: Taveira, M.; Pereira, D.M.; Sousa, C.; Ferreres, F.; Andrade, P.B.; Martins, Anabela; Pereira, J.A.; Valentão, P.
In this work were studied the phenolic composition of in vitro material (shoots, calli, and roots) of Brassica oleracea var. costata
and its antioxidant capacity. Samples were obtained in different culture medium, with distinct supplementations to verify their
influence on those parameters. Phenolic determination was achieved by HPLC-DAD. Antioxidant activity was assessed against
DPPH•. In calli and roots no phenolic compound was identified. In shoots was verified the presence of 36 compounds, which
included hydroxycinnamic acids, flavonoids (kaempferol and quercetin derivatives), and hydroxycinnamic acyl glycosides (with a
predominance of synapoyl gentiobiosides). MS liquid medium supplemented with 2 mg/L benzylaminopurine (BAP) and 0.1
mg/L...
Tipo: Article
Palavras-chave: Brassica oleracea L. var. costata DC; Shoots; Calli; Roots; Phenolic compounds; Antioxidant activity.
Ano: 2009
URL: http://hdl.handle.net/10198/3043
In vitro cultures of Brassica oleracea L. Var. Costata DC: potential plant bioreactor for antioxidant
phenolic compounds.
Provedor de dados: 188
Autores: Taveira, M.; Pereira, D.M.; Andrade, P.B.; Sousa, C.; Ferreres, F.; Martins, Anabela; Pereira, J.A.; Seabra, R.M.;
Valentão, P.
Tipo: ConferenceObject
Ano: 2008
URL: http://hdl.handle.net/10198/5579
Inflorescences of Brassicacea species as source of bioactive compounds: a comparative study
Provedor de dados: 188
Autores: Sousa, C.; Taveira, M.; Valentão, P.; Fernandes, F.; Pereira, J.A.; Estevinho, Leticia M.; Bento, Albino; Ferreres, F.;
Seabra, R.M.; Andrade, P.B.
Two Brassica oleracea varieties (B. oleracea L. var. costata DC and B. oleracea L. var. acephala) and Brassica rapa L. var. rapa
inflorescences were studied for their chemical composition and antioxidant capacity. Phenolic compounds and organic acids
profiles were determined by HPLC–DAD and HPLC–UV, respectively. B. oleracea var. costata and B. oleracea L. var. acephala
inflorescences presented a similar qualitative phenolic composition, exhibiting several complex kaempferol derivatives and
3-p-coumaroylquinic acid, while B. rapa var. rapa was characterized by kaempferol and isorhamnetin glycosides and several
phenolic acids derivatives. B. oleracea L. var. costata and B. rapa var. rapa showed the highest phenolics content. The three
Brassica exhibited the...
Tipo: Article
Palavras-chave: Brassica oleracea L. var. costata DC; Brassica oleracea L. var. acephala; Brassica rapa var. rapa L.;
Inflorescences; Phenolic compounds; Organic acids; Antioxidant activity; Antimicrobial activity.
Ano: 2008
URL: http://hdl.handle.net/10198/757
Influence of two fertilization regimes on the amounts of organic acids and phenolic compounds of
tronchuda cabbage (Brassica oleracea L. var. costata DC).
Provedor de dados: 188
Autores: Sousa, C.; Valentão, P.; Rangel, Joana; Lopes, Graciliana; Pereira, J.A.; Ferreres, F.; Seabra, R.M.; Andrade, P.B.
A phytochemical study was undertaken on tronchuda cabbage ( Brassica oleracea L. var. costata DC) cultivated under
conventional and organic practices and collected at different times. Six organic acids (aconitic, citric, ascorbic, malic, shikimic,
and fumaric acids) were identified and quantified by HPLC-UV. Qualitative and quantitative differences were noted between
internal and external leaves. Analysis of the phenolics of the internal leaves was achieved by HPLC-DAD, and the phenolic
profile obtained was revealed to be distinct from that of the external leaves. By this means were identified and quantified 11
compounds: 3- p-coumaroylquinic acid, kaempferol 3-O-sophoroside-7-O-glucoside, kaempferol 3- O-(caffeoyl)sophoroside-7O-glucoside, kaempferol 3-...
Tipo: Article
Palavras-chave: Tronchuda cabbage; Brassica oleracea L. var. costata DC; Organic acids; Phenolic compounds; Organic and
conventional production.
Ano: 2005
URL: http://hdl.handle.net/10198/733
Metabolic and bioactivity insights into Brassica oleracea var. acephala
Provedor de dados: 188
Autores: Ferreres, F.; Fernandes, F.; Sousa, C.; Valentão, P.; Pereira, J.A.; Andrade, P.B.
Seeds of Brassica oleracea var. acephala (kale) were analyzed by HPLC/UV-PAD/MSn-ESI. Several phenolic acids and flavonol
derivatives were identified. The seeds of this B. oleracea variety exhibited more flavonol derivatives than those of tronchuda
cabbage (Brassica oleracea var. costata), also characterized in this paper. Quercetin and isorhamnetin derivatives were found only
in kale seeds. Oxalic, aconitic, citric, pyruvic, malic, quinic, shikimic, and fumaric acids were the organic acids present in these
matrices, malic acid being predominant in kale and citric acid in tronchuda cabbage seeds. Acetylcholinesterase (AChE) inhibitory
activity was determined in aqueous extracts from both seeds. Kale leaves and butterflies, larvae, and excrements of Pieris...
Tipo: Article
Palavras-chave: Brassica oleracea L. var. acephala; Brassica oleracea L. var. costata; Seeds; Pieris bassicae; Phenolic
compounds; Organic acids.
Ano: 2009
URL: http://hdl.handle.net/10198/3109
Metabolic profiling and biological capacity of Pieris brassicae fed with kale (Brassica oleracea L. var.
acephala).
Provedor de dados: 188
Autores: Ferreres, F.; Fernandes, F.; Oliveira, J.M.A.; Valentão, P.; Pereira, J.A.; Andrade, P.B.
Phenolic and organic acid profiles of aqueous extracts from Pieris brassicae material and the host kale (Brassica oleracea L. var.
acephala) leaves were determined by HPLC/UV–DAD/MSn-ESI and HPLC–UV, respectively. The identified phenolics included
acylated and nonacylated flavonoid glycosides, hydroxycinnamic acyl gentiobiosides, and sulphate phenolics. Kale exhibited the
highest content (11 g/kg lyophilized extract), while no phenolics were identified in the butterflies or exuviae. Nine different
organic acids were characterized in the materials, with kale showing the highest amount (112 g/kg lyophilized extract). With the
exception of the exuviae extract, the rest were screened for bioactivity. Using spectrophotometric microassays, all exhibited...
Tipo: Article
Palavras-chave: Pieris brassicae L.; Brassica oleracea L. var. acephala; Phenolic compounds; Organic acids; Biological activity.
Ano: 2009
URL: http://hdl.handle.net/10198/3103
Metabolic profiling and biological capacity of Pieris brassicae fed with kale (Brassica oleracea L. Var.
Acephala).
Provedor de dados: 188
Autores: Fernandes, F.; Ferreres, F.; Oliveira, J.; Valentão, P.; Pereira, J.A.; Seabra, R.M.; Andrade, P.B.
Vegetables of the Brassica group are the most commonly grown and consumed worldwide. Food plants with apparent cancer and
cardiovascular di sease-preventing properties include several varieties of Brassica oleraceae. The majority of the herbivorous
insect species are specialized feeders, for which the behavioral decision to accept a plant as food or oviposition substrate is mainly
related with sensory information. Pieris insects (Lepidoptera: Pieridae) are specialist herbivores of cruciferous plants.
Tipo: ConferenceObject
Ano: 2008
URL: http://hdl.handle.net/10198/5362
Phenolic compounds in external leaves of tronchuda cabbage (Brassica oleracea L. var. costata DC)
Provedor de dados: 188
Autores: Ferreres, F.; Valentão, P.; Llorach, R.; Pinheiro, C.; Cardoso, L.; Pereira, J.A.; Seabra, R.M.; Andrade, P.B.
Glycosylated kaempferol derivatives from the external leaves of tronchuda cabbage ( Brassica oleracea L. var. costataDC)
characterized by reversed-phase HPLC-DAD-MS/MS-ESI were kaempferol 3- Osophorotrioside- 7-O-glucoside, kaempferol 3-O(methoxycaffeoyl/caffeoyl)sophoroside-7-
O-glucoside,
kaempferol
3-O-sophoroside-7-O-glucoside,
kaempferol
3-O-sophorotrioside-7-O-sophoroside, kaempferol 3- O-sophoroside-7- O-sophoroside, kaempferol 3- O-tetraglucoside-7O-sophoroside,
kaempferol
3-
O-(sinapoyl/caffeoyl)sophoroside-7-O-glucoside,
kaempferol
3-O-(feruloyl/caffeoyl)sophoroside-7- O-glucoside, kaempferol 3-O-sophorotrioside, kaempferol 3-O-(sinapoyl)sophoroside,
kaempferol 3-O- (feruloyl)sophorotrioside, kaempferol 3- O-(feruloyl)sophoroside, kaempferol...
Tipo: Article
Palavras-chave: Tronchuda cabbage; Brassica oleracea L. var. costata DC; Kaempferol derivatives; HPLC-DAD.
Ano: 2005
URL: http://hdl.handle.net/10198/729
Phenolic profile in the quality control of walnut (Juglans regia L.)leaves
Provedor de dados: 188
Autores: Amaral, J.S.; Seabra, R.M.; Andrade, P.B.; Valentão, P.; Pereira, J.A.; Ferreres, F.
Qualitative and quantitative determinations of phenolic compounds were carried out on walnut leaves samples from six different
cultivars, with the same agricultural, geographical and climatic conditions. The evolution of major phenolic compounds amounts
was monitored from May to September. Two extractive procedures were assayed and best results were obtained using acidified
water and a solid phase extraction column purification step. Qualitative analysis was performed by HPLC-DAD/MS and, in all
samples, seven phenolic compounds were identified (3-caffeoylquinic, 3-p-coumaroylquinic and 4-p-coumaroylquinic acids,
quercetin 3-galactoside, quercetin 3-arabinoside, quercetin 3-xyloside, quercetin 3-rhamnoside) and two other partially identified
phenolics...
Tipo: Article
Palavras-chave: Juglans regia L.; Walnut leaf; Phenolic compounds; HPLC-DAD; HPLC-DAD/MS-ESI.
Ano: 2004
URL: http://hdl.handle.net/10198/629
Phenolic profiles of Portuguese olive fruits (Olea europaea L.): influence of cultivar and geographical
origin
Provedor de dados: 188
Autores: Vinha, A.F.; Ferreres, F.; Silva, B.M.; Valentão, P.; Gonçalves, Artur; Pereira, J.A.; Oliveira, M.B.P.P.; Seabra, R.M.;
Andrade, P.B.
The phenolic compounds present in 29 samples of olive fruits were analysed by reversed-phase HPLC/DAD and/or HPLC-DAD/
ESI-MS/MS. All samples were collected during the normal picking period for olive oil production, in north and central Portugal,
and were obtained from 18 different olive cultivars. Two different extraction methods were necessary for the complete
quantification of phenolic compounds, a methanolic extraction and an extraction which included a solid-phase extraction (SPE)
cleaning step. The analyses showed that all samples presented a similar profile, which included at least six identified phenolic
compounds: hydroxytyrosol, luteolin 7-O-glucoside, oleuropein, rutin, apigenin 7-O-glucoside and luteolin. Several samples also
contained...
Tipo: Article
Palavras-chave: Olea europaea L.; Olive fruit; Portuguese olive cultivars; Anthocyanins; Phenolics; HPLC-DAD;
HPLC-DAD/MS/MS/ESI.
Ano: 2005
URL: http://hdl.handle.net/10198/727
Phenolics metabolism in insects: Pieris brassicae - Brassica oleracea var. costata ecological duo
Provedor de dados: 188
Autores: Ferreres, F.; Fernandes, F.; Pereira, D.M.; Pereira, J.A.; Valentão, P.; Andrade, P.B.
Changes in the phenolics composition of Pieris brassicae larvae fasted for distinct periods (1, 2, 4, 6, and 8 h) and their excrements
and of Brassica oleracea L. var. costata DC leaves were determined by high-pressure liquid chromatography/UV-photo diode
array detector/mass spectrometry- electrospray ionization. This is the first report following phenolics’ metabolism by P. brassicae
through time. The results evidence that P. brassicae sequesters and metabolizes the phenolic compounds from the host plant. In a
general way, deacylation was the main metabolic reaction that took place, but deglycosylation and sulfate conjugation reactions
also occur. Additionally, several kaempferol derivatives containing rhamnose, which is not common in Brassica, were found...
Tipo: Article
Palavras-chave: Pieris brassicae L. larva; Brassica oleracea L. var. costata DC; Phenolics; Metabolism.
Ano: 2009
URL: http://hdl.handle.net/10198/3107
Pieris brassicae inhibits xanthine oxidase.
Provedor de dados: 188
Autores: Sousa, C.; Pereira, D.M.; Valentão, P.; Ferreres, F.; Pereira, J.A.; Seabra, R.M.; Andrade, P.B.
The antioxidant potential of an aqueous extract obtained from Pieris brassicae larvae reared on Brassica oleracea L. var. costata
DC was evaluated against 2,2-diphenyl-1-picrylhydrazyl radical and several reactive oxygen species. The results revealed an
effective concentration-dependent protective activity against superoxide and hydroxyl radicals, being superior to that of the host
plant. In addition, the larvae extract also exhibited a strong inhibitory effect on xanthine oxidase that was not observed for B.
oleracea var. costata. A weak scavenging ability was noticed for hypochlorous acid. Several phenolic compounds with complex
chemical structures that are hard to synthesize in the laboratory were found in P. brassicae extract. This is the first time...
Tipo: Article
Palavras-chave: Pieris brassicae; Brassica oleracea var. costata; Phenolic compounds; Antioxidant activity.
Ano: 2009
URL: http://hdl.handle.net/10198/3102
Reactive nitrogen species scavenging by tronchuda cabbage.
Provedor de dados: 188
Autores: Sousa, C.; Guerra, L.; Valentão, P.; Ferreres, F.; Pereira, J.A.; Seabra, R.M.; Andrade, P.B.
Tipo: ConferenceObject
Ano: 2007
URL: http://hdl.handle.net/10198/5376
Rumex induratus leaves: interesting dietary source of potencial bioactive compounds
Provedor de dados: 188
Autores: Ferreres, F.; Ribeiro, V.; Gil Izquierdo, A.; Rodrigues, M.A.; Seabra, R.M.; Andrade, P.B.; Valentão, P.
The phenolic compounds of Rumex induratus leaves were determined by HPLC-DAD-MS/MS-ESI. The results revealed a profile
composed of 19 compounds: caffeoyl-hexoside, two p-coumaroyl-hexoside isomers, feruloyl-hexoside, sinapoyl-hexoside,
6-C-hexosyl-quercetin,
8-C-hexosyl-luteolin,
3-O-rutinosyl-quercetin,
6-C-hexosyl-luteolin,
7-O-hexosyl-diosmetin,
6-C-hexosyl-apigenin,
3-O-rutinosyl-isorhamnetin,
3-O-hexosyl-quercetin,
7-O-(acetyl)-pento-hexosyl-diosmetin,
6-C-hexosyl-genkwanin, and four unidentified O-glycosyl-C-glycosylflavones. The quantification of the identified phenolics by
HPLC-DAD showed 6-C-hexosyl-luteolin as the main compound. Organic acid composition was determined by HPLC-UV,
revealing a high content of oxalic acid. R. induratus was also...
Tipo: Article
Palavras-chave: Rumex induratus; Phenolic compounds; Oxalic acid; Antioxidant activity.
Ano: 2006
URL: http://hdl.handle.net/10198/2200
Rumex induratus leaves: phenolic compounds and antioxidant capacity
Provedor de dados: 188
Autores: Valentão, P.; Ribeiro, Vitor; Lopes, Graciliana; Rodrigues, M.A.; Izquierdo, Angel; Ferreres, F.; Seabra, R.M.
The phenolic composition of Rumex induratus was determined by HPLC-DAD-MS/MS-ESI and HPLC/DAD. The results showed
a profile composed by caffeoyl-hexoside. two p-coumaroyl-hexoside isomers, feruloyl-hexoside, sinapoyl-hexoside.
6-C-hexosyl-quercetin.
8-C-hexosyl-luteolin,
3-O-rutinosyl-quercetin.
6-C-hexosyl-
7.O.hexosyI-diosnietin,
luteolin,
6.C-hexosyl-apigenin.
3.O-rutinosyl-isorhamnetin,
3-O-
hexosyl-quercetin,
7-0.(acetyl)-pentohexosyl-diosmetin,
6-C-hexnsyl-genkwanin and four unidentified O.glycosyl_C-glycosylflavones. R. induratus was also investigated for its capacity
to act as a scavenger of DPPH and superoxide radicals. Good antioxidative results were obtained against both radicals.
Tipo: ConferenceObject
Ano: 2006
URL: http://hdl.handle.net/10198/2947
Screening of antioxidant compounds during sprouting of Brassica oleracea L. var. costata DC
Provedor de dados: 188
Autores: Sousa, C.; Lopes, Graciliana; Pereira, D.M.; Taveira, M.; Valentão, P.; Seabra, R.M.; Pereira, J.A.; Baptista, Paula;
Ferreres, F.; Andrade, P.B.
The changes in antioxidant compounds of Brassica oleracea L. var. costata DC seeds were monitored during the first twelve days
of seedling development. Sprouts were screened at time intervals of two days for phenolic compounds and organic acids. The
identified phenolic compounds included esters of sinapic acid with glucose, gentiobiose and kaempferol, as well as
sinapoylcholine. The organic acids were oxalic, aconitic, citric, pyruvic, malic, shikimic, and fumaric acids. During germination, a
depletion of phenolic compounds was observed, although no qualitative changes were seen. Among individual compounds,
kaempferol, choline and glucose esters of sinapic acid showed a marked decrease between days two and six, whereas the changes
in gentiobiose esters of...
Tipo: Article
Palavras-chave: Brassica oleracea L. var. costata DC; Sprouts; Phenolic compounds; Organic acids.
Ano: 2007
URL: http://hdl.handle.net/10198/720
Screening of antioxidant phenolic compounds produced by in vitro shoots of Brassica oleracea L. var.
costata DC.
Provedor de dados: 188
Autores: Ferreres, F.; Sousa, C.; Pereira, D.M.; Valentão, P.; Taveira, M.; Martins, Anabela; Pereira, J.A.; Seabra, R.M.;
Andrade, P.B.
The phenolic compounds produced by in vitro shoots of Brassica oleracea L. var. costata DC were screened by
HPLC-DAD-MS/MS. Thirty seven compounds were characterized, which included chlorogenic acids, flavonoids (the majority of
them were hydroxycinnamic acid esters of kaempferol and quercetin glycosides) and hydroxycinnamic acyl glycosides (with
predominance of synapoyl gentiobiosides). The antioxidant capacity of the shoots was assessed against DPPH radical and two
reactive oxygen species (superoxide radical and hypochlorous acid). A strong concentrationdependent antioxidative capacity was
verified in the DPPH and superoxide radicals assays, but a reduced effect was noticed against hypochlorous acid. The results
obtained indicate that the in vitro...
Tipo: Article
Palavras-chave: Brassica oleracea L. var. costata DC; In vitro Shoots Phenolic compounds Antioxidant activity.
Ano: 2009
URL: http://hdl.handle.net/10198/3045
Targeted metabolite analysis and antioxidant potential of rumex induratus
Provedor de dados: 188
Autores: Guerra, Luís; Pereira, C.; Rodrigues, M.A.; Andrade, P.B.; Ferreres, F.
Targeted metabolite analysis of aqueous extract of Rumex induratus leaves, in terms of phenolic compounds and organic acids,
and the study of its antioxidant activity against the DPPH• radical, a reactive oxygen species, hypochlorous acid, and a reactive
nitrogen species, nitric oxide (•NO), were performed. The samples were collected in several locations, spontaneously occurring or
from greenhouse culture, at different stages of development and seasons. The phenolic composition was achieved by
high-performance liquid chromatography (HPLC)-diode array detection, and four hydroxycinnamic acid derivatives and 10
flavonoid glycosides (C- and O-heterosides) were determined. Organic acids composition was established by HPLC-UV,
revealing five compounds. The...
Tipo: Article
Palavras-chave: Rumex induratus; Phenolic compounds; Organic acids; Antioxidant activity.
Ano: 2008
URL: http://hdl.handle.net/10198/2197
Targeted metabolite analysis and biological activity of Pieris brassicae fed with B. rapa var. rapa.
Provedor de dados: 188
Autores: Pereira, D.M.; Noites, A.; Valentão, P.; Ferreres, F.; Pereira, J.A.; Vale-Silva, Luís A.; Pinto, Eugénia; Andrade, P.B.
For the first time, an insect-plant system, Pieris brassicae fed with Brassica rapa var. rapa, was tested for its biological capacity,
namely, antioxidant (DPPH•, •NO, and O2 •- radicals) and antimicrobial (bacteria and fungi) activities. Samples from the insect’s
life cycle (larvae, excrements, exuviae, and butterfly) were always found to be more efficient than the host plant. Also, P.
brassicae materials, as well as its host plant, were screened for phenolics and organic acids. The host plant revealed higher
amounts of both compounds. Two phenolic acids, ferulic and sinapic, as well as kaempferol 3-Osophoroside, were common to
insect (larvae and excrements) and plant materials, with excrements being considerably richer. Detection of sulfated compounds
in...
Tipo: Article
Palavras-chave: Brassica rapa var. rapa; Pieris brassicae; Plant-insect interactions; Phenolic compounds; Organic acids;
Antioxidant activity.
Ano: 2009
URL: http://hdl.handle.net/10198/4560
Tronchuda cabbage (Brassica oleracea L. var. costata DC) seeds: phenolic compounds and
antioxidant potential.
Provedor de dados: 188
Autores: Andrade, P.B.; Sousa, C.; Seabra, R.M.; Pereira, J.A.; Ferreres, F.
Tronchuda cabbage (Brassica oleracca L. var. "oslalo DC) seeds were studied for their phenolic composition and antioxidant
capacity. Thirteen compounds were characterized and quantified by reversed-phase HPLC-OAO-MSIMSESI and HPLC-OAD,
respectively: two sinapoylgentiobiose isomers, three sinapoylglucosc isomers, kacmpferol-3- (sinapoyl)sophofOtrioside-7 -gI
ucosidc, sinapoyIchol inc; kaempferol-3, 7-diglucoside-4' -(sinapoyl)glucoside, three disinDpoylgentiobiose isomers, 1,2,2'
-trisinapoyIgentiobiose and I ,2-disinapoylglucosc. The aqueous extract of tronchuda cabbage seeds WDS investigated for its
capacity to act as a scavenger of DPPH radical and reactive oxygen species (superoxide and hydroxyl radicals Dnd hypochlorous
acid), exhibiting antioxidant...
Tipo: ConferenceObject
Ano: 2006
URL: http://hdl.handle.net/10198/5373
Tronchuda cabbage (Brassica oleracea L. var. costata DC) seeds: phytochemical characterization and
antioxidant potential.
Provedor de dados: 188
Autores: Ferreres, F.; Sousa, C.; Valentão, P.; Seabra, R.M.; Pereira, J.A.; Andrade, P.B.
Tronchuda cabbage (Brassica oleracea L. var. costata DC) seeds were studied for their chemical composition and antioxidant
capacity. Thirteen phenolic compounds were characterized and quantified by reversed-phase HPLC-DAD-MS/MS-ESI and
HPLC-DAD,
respectively:
two
sinapoylgentiobiose
isomers,
three
sinapoylglucose
isomers,
kaempferol-3-(sinapoyl)sophorotrioside-7-glucoside, sinapoylcholine, kaempferol-3,7-diglucoside-40-(sinapoyl)glucoside, three
disinapoylgentiobiose isomers, 1,2,20-trisinapoylgentiobiose and 1,2-disinapoylglucose. Seven organic acids (aconitic, citric,
ascorbic, malic, quinic, shikimic and fumaric acids) were also identified and quantified by HPLC-UV. The aqueous extract of
tronchuda cabbage seeds was investigated for its capacity to act...
Tipo: Article
Palavras-chave: Tronchuda cabbage seeds; Brassica oleracea L. var. costata DC; Phenolic compounds; Organic acids;
Antioxidant capacity.
Ano: 2007
URL: http://hdl.handle.net/10198/752
Tronchuda cabbage flavonoids uptake by Pieris brassicae
Provedor de dados: 188
Autores: Ferreres, F.; Sousa, C.; Valentão, P.; Pereira, J.A.; Seabra, R.M.; Andrade, P.B.
The flavonoid pattern of larvae of cabbage white butterfly (Pieris brassicae L.; Lepidoptera: Pieridae) reared on the leaves of
tronchuda cabbage was analysed by HPLC-DAD-MS/MS-ESI. Twenty flavonoids were identified or characterised, namely 16
kaempferol and four quercetin derivatives. Kaempferol 3-O-sophoroside, a minor component of tronchuda cabbage, was found to
be the main component in P. brassicae (15.8%). Apart from this, only two other flavonoids present in significant amounts in
tronchuda cabbage (kaempferol 3-O-sophoroside-7-O-glucoside and kaempferol 3-O-sophoroside-7-O-sophoroside) were found
in the larvae. The larvae have high amounts of quercetin derivatives (18.5%), which were present only in trace amounts in
tronchuda cabbage extracts,...
Tipo: Article
Palavras-chave: Pieris brassicae L. larvae; Tronchuda cabbage; Brassica oleracea L. var. costata DC; Flavonoids.
Ano: 2007
URL: http://hdl.handle.net/10198/745
Walnut (Juglans regia L.) leaves: phenolic compounds, antimicrobial activity ans antioxidant
potential of different cultivars.
Provedor de dados: 188
Autores: Pereira, J.A.; Oliveira, I.; Sousa, A.; Valentão, P.; Andrade, P.B.; Ferreira, Isabel C.F.R.; Ferreres, F.; Bento, Albino;
Seabra, R.M.; Estevinho, Leticia M.
Different cultivars of walnut (Juglans regia L.) leaves (Cv. Lara, Franquette, Mayette, Marbot, Mellanaise and Parisienne) grown
in Portugal, were investigated in what concerns phenolic compounds and antimicrobial and antioxidant properties. Phenolics
analysis was performed by reversed-phase HPLC/DAD and 10 compounds were identified and quantified: 3- and 5-caffeoylquinic
acids, 3- and 4-pcoumaroylquinic acids, p-coumaric acid, quercetin 3-galactoside, quercetin 3-pentoside derivative, quercetin
3-arabinoside, quercetin 3-xyloside and quercetin 3-rhamnoside. The antimicrobial capacity was screened against Gram positive
(Bacillus cereus, B. subtilis, Staphylococcus aureus) and Gram negative bacteria (Pseudomonas aeruginosa, Escherichia coli,
Klebsiella...
Tipo: Article
Palavras-chave: Walnut leaves; Phenolics; Antimicrobial activity; Antioxidant properties.
Ano: 2007
URL: http://hdl.handle.net/10198/719