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1 Nutrient Availability of Tea Growing Soil Influenced by 2 Different Rates of Dolomite 3 4 ABSTRACT 5 Teas (Camellia sinensis L.) exclusively prefer to grow in acid soils but in very acidic nature it is 6 detrimental to the available nutrient content especially Ca, Mg and Mn in soil. Dolomite is soil 7 amendment which used to mitigate the soil acidity and also it provides some essential nutrient Ca 8 and Mg itself. Present investigation was undertaken to identify the effect of different rate of 9 Dolomite on major and micronutrient availability of Tea growing soils of low country wet zone. 10 Field trial was laid out in Randomized Complete Block Design consisting of five treatments in 11 different rate of Dolomite (kg/ha/pruning cycle) namely; T1 (control), T2 (1000), T3 (2000), T4 12 (3000), and T5 (4000). Soil nutrient content at 0-15cm and 15-30cm of depths were studied. The 13 data generated from the study was analyzed by using Analysis of Variance (ANOVA) in SAS 14 statistical package. Treatment means were compared at probability p< 0.05 using LSD. Soil 15 Exchangeable Al, Ca and D.T.P.A extractable Mn were had no effect. But soil available Fe was 16 significantly declined according to the dolomite rate. The highest average mean value of Fe was 17 obtained in control. Highest average means of soil Exchangeable Mg (101.33mg/kg) was 18 observed in highest dolomite applied plots at 0-15cm depth and highest K (130.67mg/kg) was 19 recorded in the treatment with 2000kg/ha/pruning cycle. 20 21 Key words: Calcium, Dolomite, Iron, Magnesium 22 23 1. INTRODUCTION 24 Tea plays a major role in the economy of Sri Lanka because it is one of the major foreign 25 exchange earners to the country. It is an acceptable fact that “Ceylon tea” is recognized 26 internationally and renowned worldwide for its quality. Sri Lankan tea has traditionally ranked 27 among the world’s prime-quality teas due to its strong flavour and aroma (Sector report, 2010). It 28 contributes, 1.3% to the Gross Domestic Product (GDP) (Anon, 2011), 16.57% of the total 29 export income and 67.38% of agricultural export earnings (Anon, 2010). 30 31 Tea growing areas in Sri Lanka mainly falls in high elevated area. Elevation or altitude is one of 32 the largest local or regional influencers of climate. As one climbs to a higher elevation, 33 temperatures become more variable, rainfall generally becomes higher, but humidity becomes 34 lower. The soil of the main tea growing areas in the country undergo leaching due to rainfall, and 35 hence generally poor in cations such as K, Mg, and Ca .Tea soils are generally rich in aluminum 36 ions (Al3+) and those ions also indirectly cause soil acidity. A soil suitable for tea growing is 37 moderately acidic with pH ranging of 4.5-5.5 (Zoysa, 2008) any significant deviation from this 38 range could cause difficulties in the uptake of nutrients. The pH is very critical for nursery soil in 39 tea cultivation. The preferable pH range is 4.5-5.5 but best result is obtained near pH 5.0 40 (Kathiravetpillai and Kulasegaram, 2008). 41 42 Dlomitic limestone (CaCO3.MgCO3) is recommended for a tea soil which is provides both 43 calcium and magnesium (Tea Circular 1989). Zoysa (2002) reported that the application of 44 dolomite powder to tea soils is an important agronomic practice. This helps the maintenance of 45 sustainability of tea cultivation in Sri Lanka. The amount of dolomite required to be applied to 46 the soil depends on the soil pH and the buffering ability of the soil. Because most soil can resist 47 pH changes to an appreciable extent when large amounts of materials either acid forming or base 48 forming fertilizers added (Zoysa et al., 2008). Dolomite should be applied to pruning field soon 49 before or after the pruning, while ensuring even distribution on the ground. The beneficial effects 50 of liming are: improvement of soil pH suitable for nutrient availability and absorption, supply of 51 an inexpensive source of magnesium and calcium, reduction of possible toxicity by aluminum 52 and manganese and improvement of soil physical and biological conditions. Iron, aluminum, 53 titanium, manganese, silica, sodium, potassium and organic matter are the usual minor 54 constituents of dolomite (Pathirana, 2000). 55 56 In this context this study was carried out to develop dolomite recommendation for the mid 57 country wet zone of Sri Lanka with the specific objective of to study effect of application of 58 dolomite on major and micro nutrient availability of tea growing soils. 59 60 2. MATERIALS AND METHODS 61 2.1 Description of the Study Area 62 A field experiment was conducted in 2015 at Rathode tea estate. The site is situated in the mid 63 country wet zone of Sri Lanka at latitude of 7°31'4.44" N and longitude of 80°43'23.87".The 64 experimental site lies at an altitude of about 884 mean sea level. It is characterized by average 65 rainfall of 1250-3150 mm, soils are derived from the colluvial material , well drained and very 66 deep having dark reddish brown, clay loam surface underlain by dark reddish brown, loam 67 surface soils (Mapa.,1999). 68 2.2 Treatments and Experimental Design 69 The field experiment was carried out using the tea cultivar TRI 2023 .The Randomized complete 70 Block Design (RCBD) with 3 replicates was used as an experimental design. Field plots were 71 established for five levels of treatments. Rectangular plots of highest length were recommended 72 because of sloppiness of land. Each plot was separated by the gourd row which separated the 73 treated area in order to prevent the treatment effect in any adjacent plots. Each individual plot 74 was marked with 30 bushes. 75 2.3 Fertilizer Application 76 Fertilizer was applied to all plots evenly. Nitrogen, K2O and P2O5 applied at the rate of 320, 120 77 and 35 Kg/ha/year respectively. 78 2.4 Sampling Procedure 79 Soil samples from two depth 0-15cm and 15-30cm were collected from the randomly selected 80 places in each plot as a bulk and sub sample was taken from the bulk. During soil sampling dead 81 plant, stones, area near tree and other inert materials were removed. A part of the sub sample was 82 allowed to air dried and passed through the 2mm sieve prior to chemical analysis in order to get 83 homogeneous sample. 84 2.5 Soil Analytical methods 85 Soil sample was analyzed at laboratory of soil and plant nutrition Division, Tea Research 86 Institute, St cooms Estate Talawakella. Trace elements iron and manganese were extracted by 87 DTPA and concentration was determined by AAS. Aluminium extracted by KCl and 88 concentration was measured using spectrophotometer at 530 nm wavelength (Bertsch et al., 89 1981). Exchangeable Ca2+, Mg2+ and K+ were extracted by ammonium chloride (Blackmore et 90 al, 1987). K determined by using flame photometer. Soil Ca and Mg were determined by using 91 AAS. 92 2.6 Statistical Analysis 93 Data were subjected to an analysis of variance (ANOVA) to examine the effect of dolomite 94 limestone application on major and micro nutrient availability of tea soil. A statistical analysis 95 was conducted using Statistical Analysis System (SAS) window version 9.1 and Microsoft Excel 96 2007 package. The least significance difference (LSD) test was used to separate significantly 97 differing treatment means after main effects were found significant at P < 0.05. 98 99 3. RESULT AND DISCUSSION 100 3.1 Effect of application of Dolomite on major Nutrient content of Tea soil 101 The effect of different rate of dolomite on soil Ca, Mg and K are shown in the Table 3.1 102 respectively. Different rate dolomite did not affect significantly on availability of Ca in both 103 depths. 104 105 3.1.1. Exchangeable Calcium 106 Ca is the major element present in dolomite which mitigating the soil pH. That displaces the H+ 107 and Al ions present in soil exchangeable sites. Also the soil of an experiment area has 108 considerable buffering capacity and the pH also not significant. According to that this results 109 obviously report that Ca2+ neutralized soil acidity and had no effect on available Ca on soil. 110 3.1.2. Exchangeable Magnesium 111 At 0-15cm depth Mg concentration had significant difference. Application of 4000kg 112 dolomite/ha/pruning cycle had highest Mg concentration compare to dolomite rate of 3000 and 113 plot without dolomite (Control). Plot with 1000, 2000 kg/ha/pruning cycle dolomite had no 114 effect. Dolomite provides Mg itself and Mg also responsible for the arrest the soil pH. As 115 application of lime had high Mg saturation in soil was found by Athanase et al., (2013). 116 Mg released by applied fertilizer is a may be a reason for high Mg in control plot. Mg content at 117 15-30cm had no effect. Increasing trend of soil exchangeable Mg was found by Krishnapillai et 118 al (1992) in study with incubation of different rate of dolomite with different soil type. They 119 reported soon after incubation Mg increased in all type of soil with increasing rate of dolomite 120 and there was no change in Mg with time period. 121 3.1.3 Exchangeable Potassium 122 There was no change in K content at 0-15cm depth. But considerable change was observed in 123 concentration of K at 15-30cm depth. Highest concentration of K was observed in 124 1000kg/ha/pruning cycle applied plots. Dolomite rate of 2000, 4000, control plots did not show 125 any significant effect. Lowest K concentration was found in dolomite rate of 3000 kg/ha/pruning 126 cycle. 127 availability on acid soil But Matale, soil series showed higher base saturation than the other soils 128 and also CEC of these soil also very high (10-20 cmolc kg-1) (Jayalath et al., 1998). Because of 129 this habit of soil there was no effect on Exchangeable cations in soil. Krishnapillai et al (1992) 130 reported that the exchangeable K+ had no variation with increasing rates of dolomite application. 131 Similar findings reported by Jensen (1972) and Udo (1978). Kovacevic and Rastija (2010) have shown that liming did not affect potassium 132 133 3.2 Effect of application of Dolomite on Trace element content of Tea soil 134 3.2.1 Soil Exchangeable Aluminium (Al) 135 According to results obtained from this study, significant effect on soil exchangeable Al was not 136 observed. But there was a reducing trend was observed when increasing rate of dolomite at 0- 137 15cm depth (Table 3.2) and there was no considerable change at 15-30cm soil depth. 138 Many soil scientists showed that decreasing pH decreased Exchangeable Al concentration in soil. 139 Pathirana,( 2000) observed significant reduction of Exchangeable Al by application of dolomite 140 in acid tea soil. There are many reports about the beneficial Ca effects on the amelioration of Al - 141 toxicity in different crops growing in acid soils (Mora et al., 1999; Mora et al., 2002). Other 142 studies have shown that soil pH increases after the application of Ca amendments due to the 143 displacement of Al3+ and H+ by Ca2+ from the exchange sites into the solution (Alva and Sumner, 144 1988; Mora et al. 1999). 145 D.T.P.A Extractable Mn and Fe in soil 146 The experimental data available do not furnish evidence that the application of different date of 147 dolomite on the availability of D.T.P.A extractable Mn at both depths (Table 3.2), while lowest 148 value was recorded in dolomite rate of 4000 kg/ha/pruning cycle. 149 Soil available Fe also did not show significant variation between the treatment in 0-15cm depth 150 but the significant different was observed in 15-30 cm soil depth. Lower Fe concentration was 151 observed in dolomite applied plots than control which had high available Fe in soil. 152 4. CONCLUSION 153 This study shows the advantage of incorporating dolomite which helps to supply the soil with 154 adequate quantities of available magnesium and at the same time to reduce the concentration of 155 aluminium and to eliminate possible toxic effects of aluminium, manganese and iron in tea soils. 156 Although it was observed that the soil exchangeable K did not show much variation with 157 increasing rates of dolomite application. 158 REFERENCES 159 160 161 Alva A. K., and Sumner M. E., 1988, Effects of phosphogypsum or calcium sulfate on reactive aluminum in solutions at varying pH, Commun. Soil Sci. Plan, 19: 1715 – 1730 162 Anon., 2010, Annual report of Tea Research Institute of Sri Lanka, pp 53 163 Anon, (2011) Annual report. Central Bank of Sri Lanka 164 Athanase N., Vicky R., Jayne N. M., and Athanase C. R., 2013, Effects of Unburned Lime on 165 Soil pH and Base Cations in Acidic Soil. Research Article ISRN Soil Science .Article 166 ID 707569, http://dx.doi.org/10.1155/2013/707569 167 168 169 170 171 172 Bertsch P.M., Alley M.M., and Ellmore T.L., 1981, Automated Aluminum Analysis With The Aluminon Methods. Soil Science Society of America Journal, .45: 666-667 Blackmore L.C., Searle P.L., and Daly B.K., 1987, Methods of chemical analysis of soils, Scientific Report 80, New Zealand Soil Bureau, Lower Hutt Jayalath K.D.D., Dassanayake A.R., and Mapa R.B., 1998, Suitability of Mid Country Wet Zone Lands for Plantation Agriculture, Tropical Agricultural Research, 10: 103-116 173 174 175 176 177 178 179 180 181 Jensen H. E., 1972, Cation adsorption isotherms derived from mass-action theory, Roy.Vet.Agric Univ, Copenhagen, pp 88-103 Kovacevic V., and Rastij M., 2010, Impacts of liming by dolomite on the maize and barley grain yields, Poljoprivreda ,16 (2) : 3-8 Krishnapillai S., Jeyachandran N., and Balakrishnan T., 1992, Effect of dolomite on soil reaction and nutrient availability in tea soils. Sri Lankan journal of tea science. 61 (1): 4-14 Mapa R.B., Somasiri S., and Nagarajah S., 1999, Soils of the Wet Zone of Sri Lanka. Soil Sceince Society of Sri Lanka, pp 184 Mora M. L., Carte, P., Demanet R., and Cornfort, I.S., 2002, Effects of lime and gypsum on 182 pasture growth and composition on an acid Andisol in Chile, South America. Commun. Soil 183 Sci. Plant Anal, 33: 2069 - 2081 184 Mora M. L., Schnettler B., and Demanet R., 1999, Effect of liming and gypsum on soil 185 chemistry, yield, and mineral composition of ryegrass grown in an acidic Andisol. 186 Commun. Soil Sci. Plant Anal, 30: 1251 – 1266 187 188 Pathirana N.S.W., 2000, Application of Dolomite to tea soils Nutrient availability and estimation of dolomite residues 189 Sector Report tea industry, 2010 www.ram.com.lk 190 Udo E.J., 197,) Thermodynamics of Potassium - Calcium and Magnesium - Calcium exchange 191 192 reactions on a Kaolinitic soil clay, Soil Sci.Soc. Amer J, 42: 556-56 Zoysa A.K.N., Anandacoomaraswamy A., and De Silva M.S.D.L., 2008, Management of soil 193 Fertility in tea lands: In handbook on tea, Tea research institute of Sri Lanka, pp 27-33 194 Zoysa, A.K.N., 2002, Some Aspects of Dolomite Use in Tea Cultivation. Tea Bulletin vol 17 No 195 196 197 198 199 1 & 2 Dec 2002. The tea research institute of Sri Lanka 200 201 202 203 204 205 206 207 208 209 210 211 212 213 214 215 216 217 218 219 220 221 222 223 224 225 Table 2.1: Treatments Treatments Kg/ha/cycle Treatment (T1) Control Treatment (T2) 1000 Kg/ha/cycle Treatment (T3) 2000Kg/ha/cycle Treatment (T4) 3000Kg/ha/cycle Treatment (T5) 4000Kg/ha/cycle 226 227 Table 3.1 : Effect of application of different rate of dolomite on soil Exchangeable Ca, Mg 228 and K at 0-15cm and 15-30 cm depth 229 Level of Dolomite Major nutrient content at Major nutrient content 230 0-15cm depth at (kg/ha/pruning 231 15-30 cm depth 232 cycle) Ca Mg K Ca Mg K 233 (mg/kg (mg/kg (mg/kg) (mg/k (mg/k (mg/k g g) g) ) 234 235 236 237 0 210.33a 91.00a 66.33a 205.0a 58.33a 97.33ba 1000 220.33a 43.00b 66.33a 192.0a 43.67a 130.67 239 238 a 2000 199.33a 56.00b 86.00a 150.7a 48.33a ba 88.67 241 3000 318.33a 96.00a 100.00a 194.0a 53.33a 242b 66.67 4000 302.00a 101.33a 66.67a 251.0a 102.0a 243ba 97.33 LSD Value(<0.05% 158.82 34.323 40.415 252.22 82.93 244 59.34 245 P) 249 250 251 252 253 254 255 256 257 258 259 240 246 CV % 33.73 23.53 27.85 67.45 72.05 32.78 P Value 0.345 0.014 0.279 0.922 0.530 0.264 247 248 Means followed by the same letter in each column are not significantly different to LSD at 5% level 260 261 262 263 264 265 266 267 268 269 270 271 272 273 274 275 276 Figure 3.1: Major nutrient content at 0-15 cm depth 277 278 279 280 281 282 283 284 285 286 287 288 289 290 291 292 293 294 295 296 297 Figure 3.2: Major nutrient content at 15-30 cm depth 298 299 300 301 302 303 304 305 306 307 308 309 310 311 312 313 314 315 316 317 318 319 320 321 322 323 324 325 326 327 328 Figure 3.3: Al (mg/kg) deviation at different depth 329 Table 3.2: Effect of application of different rate of dolomite on trace elements in soil 330 Level of Dolomite Mn (mg/kg) Fe (mg/kg) Al (mg/kg) (Kg/ha/pruning cycle) 331 332 333 334 335 336 337 338 339 340 341 0-15cm 15-30cm 0-15cm 15-30cm 0-15cm 15-30cm 0 12.00a 30.00a 2.67a 3.67a 50.00a 50.33a 1000 10.67a 19.33a 1.33a 1.33ba 55.33a 14.00a 2000 9.33a 7.67a 1.33a 1.00b 43.00a 68.00a 3000 14.00a 23.33a 4.00a 2.33ba 37.33a 62.67a 4000 4.33a 11.00a 1.67a 2.33ba 24.00a 50.00a LSD Value (<0.05% P) 14.57 51.31 3.141 2.57 23.82 83.78 CV % 76.87 149.17 75.83 64.04 30.16 90.81 P value 0.634 0.848 0.313 0.231 0.103 0.629 Means followed by the same letter in each column are not significantly different to LSD at 5% level. 342 343 344 345 346 347 348 349 350 351 352 353 Figure 3.4: Fe (mg/kg) deviation at different depth 354 355 356 357 358 359 360 361 362 363 364 365 366 367 368 369 370 Figure 3.5: Mn (mg/kg) deviation at different depth 371 372 373 Figure 3.6: Overall available trace nutrients in soil