Download Supplementary Material, Table 1

Supplementary Material, Table 1
A. Recombination between homologs in hhf1-10 (4K-Q)
Recombination
H4 Allele
Mutation
Chromosome LossNo.Expts.
Frequency
HHF1
KKKK (WT)
3.9 x 10-4
2.3 x 10-4
6
hhf1-10
QQQQ
10.6 x 10-4
4.5 x 10-4
6
Diploid strains heterozygous for recessive markers on the two arms of
chromosome V were constructed. The left arm is marked with can1/CAN1 and
the right arm is marked with hom3/HOM3.The can1 allele renders haploid cells
resistant to canavanine (Canr), while hom3 makes cells auxotrophic for
methionine (Met-). The heterozygous diploid is both Cans and Met+. In this
background we compared the homozygous wild-type (HHF1/HHF1) to the
homozygous hhf1-10/hhf1-10 mutant. Mitotic crossing over on the left arm of
chromosome V was assayed by scoring the frequency of Can r Met+
recombinants.
B. Recombination between direct repeats in hhf1-10 (4K-Q)
Recombination
Recombination
Frequency
Frequency
Mutation
- MMS
+ MMS
HHF1
KKKK (WT)
0.8 x 10-3
4.7 x 10-3
2
hhf1-10
QQQQ
6.5 x 10-3
16.3 x 10-3
2
H4 Allele
No.Expts.
Supplementary Material, Table 2
All genes expressed at 2-fold or lower levels in hhf1-10
Fold
Decrease
16.4
7.3
6.6
6.6
5.4
4.9
4.4
4.0
3.6
3.6
3.3
3.3
3.3
3.3
3.0
3.0
3.0
3.0
3.0
3.0
3.0
2.7
2.7
2.7
2.7
2.7
2.7
2.7
2.7
2.7
2.7
2.7
2.4
2.4
2.4
2.4
2.4
2.4
ORF
Name
YDR380W
YHR137W
YHR136C
YDR281C
YBR085W
YER011W
YJL088W
YPR200C
YOL058W
YMR319C
YLR467W
YLR367W
YIR030C
YDL039C
YOL126C
YIL114C
YIL020C
YIL011W
YGR286C
YER069W
YCL064C
YPL241C
YPL189W
YNL125C
YMR063W
YLR466W
YHR015W
YGR029W
YGR029W
YGL255W
YBR065C
YAR071W
YNL339C
YMR006C
YML123C
YLR467W
YIL013C
YHR184W
Gene
Name
ARO10
ARO9
SPL2
PHM6
AAC3
TIR1
ARG3
ARR2
ARG1
FET4
YRF1-5
RPS22B
DCG1
PRM7
MDH2
POR2
HIS6
TIR3
BIO2
ARG5,6
CHA1
CIN2
GUP2
ESBP6
RIM9
YRF1-4
MIP6
ERV1
ERV1
ZRT1
ECM2
PHO11
YRF1-6
PLB2
PHO84
YRF1-5
PDR11
SSP1
Description
Hypothetical ORF
Aromatic amino acid aminotransferase II
Suppressor of plc1-
Phosphate metabolism; transcription is regulated b
Highly homologous to PET9 (AAC2) and AAC1
Cold-shock induced protein of the Srp1p/Tip1p fa
Sixth step in arginine biosynthesis
Required for arsenate but not for arsenite resistanc
Arginosuccinate synthetase
Putative transmembrane low-affinity Fe(II) transp
Y'-helicase protein 1
Homology to rat S15a
Product of gene unknown
Pheromone-regulated membrane protein
Cytosolic malate dehydrogenase
Mitochondrial porin
Phosphoribosyl-5-amino-1-phosphoribosyl-4-imid
TIP1-related
Biotin synthase
N-acetyl-gamma-glutamyl-phosphate reductase an
Catabolism of hydroxy amino acids
Involvement in microtubule function
Involved in active glycerol uptake
Protein with similarity to mammalian monocarbox
Regulator of Rim1p, required for IME1 expression
Y'-helicase protein 1
RNA-binding protein, interacts with MEX67
Protein essential for mitochondrial biogenesis and
Protein essential for mitochondrial biogenesis and
High-affinity zinc transport protein
ExtraCellular Mutant
Acid phosphatase, secreted
Y'-helicase protein 1
Phospholipase B 2
Inorganic phosphate transporter, transmembrane p
Y'-helicase protein 1
ATP-dependent permease, member of ATP-bindin
Involved in the control of meiotic nuclear division
for mitosis, premeiotic DNA synthesis, recombina
initiation of prospore walls; required for spore wa
2.4
2.4
2.4
2.4
2.4
2.2
2.2
2.2
2.2
2.2
2.2
2.2
2.2
2.2
2.2
2.2
2.2
2.2
2.2
2.2
2.2
2.0
2.0
2.0
2.0
2.0
2.0
2.0
2.0
2.0
2.0
2.0
2.0
2.0
2.0
2.0
2.0
2.0
2.0
YHL028W
YGR177C
YER091C
YDR044W
YBR213W
YPR069C
YOR377W
YOR336W
YOR011W
YLR130C
YJR147W
YIL162W
YHR094C
YFR036W
YER157W
YDR538W
YDR021W
YDL205C
YDL049C
YBR093C
YAR073W
YPR176C
YPL122C
YOR229W
YOL143C
YOL102C
YNL220W
YKL132C
YGR029W
YGL027C
YFR025C
YER063W
YEL056W
YDR213W
YDL198C
YDL182W
YBR145W
YBL068W
YAR031W
WSC4
ATF2
MET6
HEM13
MET8
SPE3
ATF1
KRE5
AUS1
ZRT2
HMS2
SUC2
HXT1
CDC26
SEC34
PAD1
FAL1
HEM3
KNH1
PHO5
IMD1
BET2
TFB2
WTM2
RIB4
TPT1
ADE12
RMA1
ERV1
CWH41
HIS2
THO1
HAT2
UPC2
YHM1
LYS20
ADH5
PRS4
PRM9
Cell wall integrity and stress response component
Alcohol acetyltransferase
Vitamin B12-(cobalamin)-independent isozyme o
Oxygen-repressed, sixth step in heme biosynthetic
Protein involved in the expression of PAPS reduct
Biosynthesis of spermidine
Alcohol acetyltransferase
Appears to function early in (1,6)-beta-D-glucan s
ABC (ATP-binding cassette) protein involved in U
Low-affinity zinc transport protein
High-copy mep2 suppressor
Invertase (sucrose hydrolyzing enzyme)
High-affinity hexose (glucose) transporter
Component of anaphase-promoting complex
Secretion (golgi retention) deficient
Phenylacrylic acid decarboxylase
Similar to eukaryotic initiation factor eIF4a
Catalyzes the third step in heme biosynthesis
46% identical at amino acid level to Kre9p
Acid phosphatase, repressible
IMP dehydrogenase homolog
Geranylgeranyltransferase Type II beta subunit
Transcription/repair factor TFIIH subunit
WD repeat containing transcriptional modulator 2
Catalyzes synthesis of immediate precursor to ribo
Gene encodes a protein implicated in the last step
Adenylosuccinate synthetase
Similar to folyl-polyglutamate synthase
Protein essential for mitochondrial biogenesis and
Glucosidase I, involved in assembly of cell wall b
Histidinolphosphatase
Suppressor of the Transcriptional (T) defect of Hp
Subunit of histone acetyltransferase
Involved in sterol uptake
High copy suppressor of abf2
Homocitrate synthase
Alcohol dehydrogenase isoenzyme V
Ribose-phosphate pyrophosphokinase 4
Pheromone-regulated membrane protein