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Download Sp1 interacted proteins in mitotic stage
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Supplementary Table 1. Proteins interact with Sp1 in mitotic stage. Cells were synchronized in mitosis, and cell lysates were harvested for immunoprecipitation assay with anti-Sp1 antibodies. Protein identification was performed by LTQ-ObiTrap (MASS). All of the identified proteins shown here have 5 fragments at least to be identified, and Sp1 determined by immunoblotting with anti-Sp1 antibodies was as an internal control. Gene name ACTG ACTBL ACTC ANXA2 CALM CAPZB CBP CC29L CNTLN CO3A1 CS021 DESM DREB FKB15 FGOP2 GBP1 GRP75 LMO7 LUZP1 MAT1 MRCKB MYH7B MYH9 MYH10 MYH11 MYH14 MYO1B MYO1C MYO1E MYO6 ML12A MYL9 MYL6B MYL3 Protein name Actin, cytoplasmic 2 Beta-actin-like protein 2 Actin, alpha cardiac muscle 1 Annexin A2 Calmodulin F-actin-capping protein subunit beta CREB-binding protein Putative coiled-coil domain-containing protein 29-like Centlein Collagen alpha-1(III) chain Uncharacterized protein c19 or f21 Desmin Drebrin FK506-binding protein 15 FGFR1 oncogene partner 2 Interferon-induced guanylate-binding protein 1 Stress-70 protein, mitochondrial LIM domain only protein 7 Leucine zipper protein 1 CDK-activating kinase assembly factor MAT1 Serine/threonine-protein kinase MRCK beta Myosin-7B Myosin-9 Myosin-10 Myosin-11 Myosin-14 Myosin-Ib Myosin-Ic Myosin-Ie Myosin-VI Myosin regulatory light chain 12A Myosin regulatory light polypeptide 9 Myosin light chain 6B Myosin light chain 3 NEBU ODFP2 PLEC1 POTEE RSF1 SLIM1 SRRT TBA1B TBB3 TBB5 TIM TITIN TNR11 TPM1 TPM2 TPM3 TPM3L TPM4 VIME Nebulin Outer dense fiber protein 2 Plectin-1 POTE ankyrin domain family member E Remodeling and spacing factor 1 SLIT and NTRK-like protein 1 Serrate RNA effector molecule homolog Tubulin alpha-1B chain Tubulin beta-3 Tubulin beta-5 chain Protein timeless homolog Titin Tumor necrosis factor receptor superfamily member 11A Tropomyosin alpha chain Tropomyosin beta chain Tropomyosin alpa-3 chain Putative tropomyosin alpha-3 chain Tropomyosin alpha-4 chain Vimentin Note: Search Parameters; Type of search: MS/MS Ion Search; Enzyme: Trypsin; Mass values: Monoisotopic; Protein Mass: Unrestricted; Peptide Mass Tolerance: ± 1 Da; Fragment Mass Tolerance: ± 1 Da; Max Missed Cleavages: 2; Instrument type: ESI-TRAP; Number of queries: 1826
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