Download Figures (Replenishment of the Enteric Nervous System, Katarina

Figures: Replenishment of the Enteric Nervous System, Katarina Sandgren
Figure 1. Culturing of donor cells. A) Intestinal cells are dissociated and cultured in
medium promoting the growth of ENS progenitor cells. B) Shows neural crest derived cells
(green) after 3 weeks in culture. Blue (DAPI) demonstrates the total population of cultured
cells. A large proportion of the cultured cells express Sox10 (characterising neural
progenitors). C) The relative proportion of EPCs increased during culture.
Figure 2. ENS progenitor cells grafted into
postnatal gut. Grafted cells shown in green could
be found at the level of the myenteric (A) as well
as the submucosal plexus (B).
Figure 3. EPCs grafted into the
basis of the caecum (A, B) of
Ret-mutant gut. After 4 days in
vivo the grafted EPCs have
colonised the entire caecum (B).
C shows grafted neurons (green)
that have integrated within the
endogenous neuronal plexus (red) in wild-type recipient gut.
Figure 4. A) Ultrasound-guided microinjection
into mouse embryo gut wall at E12.5. B) 36
hours after microinjection GFP+ grafted cells
have colonised the gut of the host embryo.
Figure 5. Phase holographic images
of enteric neurons after 6 days in
culture. Characteristic morphological
features, such as size, width,
thickness, irregularity and protrusions
can be measured. In this image the
varying thickness of cells is
represented by different colours.