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AP BIOLOGY REVIEW DNA, PROTEIN SYNTHESIS, BACTERIA, VIRUSES Scientists (10): Watson & Crick Wilkins & Franklin Nirenberg Meselson & Stahl Lederberg & Zinder Lederberg & Tatum Hershey & Chase Griffith Garrod Chargaff Beadle & Tatum Avery, McLeod, McCarty Enzymes (6): Primase DNA polymerase RNA polymerase Helicase Topoisomerase endonucleases/restriction enzymes reverse transcriptase ligase peptidyl transferase activating enzymes DNA structure & replication (15): X-ray diffraction provided what information? DNA dimensions in angstroms Shape? Strands? Building blocks? Bonds? 5’ and 3’ designations purines and pyrimidines (examples, general structure, percentages) nucleotides, gene, genetic code, mutations replication: enzymes, initiation, steps, Okazaki fragments Transcription & Translation (18): definitions, steps Amino acid sequence DNA nucleotide sequence; mRNA amino acid; DNA amino acid Requirements for protein synthesis Relationship between # of amino acids in a polypeptide & # of nucleotides Prokaryotic cells? Polyribosomes Ribosome structure Types, functions & processing of RNA Bacteria & Viruses (9): Operon – definition, components, transcription Prophage, lytic cycle, lysogenic cycle Viral structure (nucleic acid? Capsid? Envelope?) Bacterial genes: structural, regulatory, constitutive Repressor protein Lac operon & trp operon (inducible, repressible, product, substrate, repressor