Download Table S3.

Table S3. Kinetic parameters and derived parameters used in the OLE network model.
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Symbol
 r ,a
Quantity
ADR1 mRNA half-life
Estimated value
22 min
 r ,c
CTA1 mRNA half-life
17 min
 r ,o
OAF1 mRNA half-life
15 min
 r,y
OAF3 mRNA half-life
22 min
 r, p
PIP2 mRNA half-life
22 min
 p, a
Adr1 protein half-life
180 min
 p,c
Cta1 protein half-life (oleate)
480 min
 p,o
Oaf1 protein half-life
54.6 min
 p, y
Oaf3 protein half-life
144 min
 p, p
Pip2 protein half-life
~40 min
rd , a
ro,c
rd ,o
ADR1 mRNA conc, glucose
CTA1 mRNA conc, oleate
OAF1 mRNA conc, glucose
0.25 molec/cell
76.7 molec/cell
0.6 molec/cell
rd , y
OAF3 mRNA conc, glucose
0.4 molec/cell
rd , p
ro ,a / rd ,a
PIP2 mRNA conc, glucose
ADR1 mRNA ratio, glycerol/glucose
CTA1 mRNA ratio, glycerol/glucose
OAF1 mRNA ratio, glycerol/glucose
OAF3 mRNA ratio, glycerol/glucose
PIP2 mRNA ratio, glycerol/glucose
ADR1 mRNA ratio, oleate/glucose
0.3 molec/cell
3.78
11.5
0.9794
1.43
2.13
4.75
ro ,c / rd ,c
CTA1 mRNA ratio, oleate/glucose
79.8
ro ,o / rd ,o
OAF1 mRNA ratio, oleate/glucose
0.9
ro , y / rd , y
OAF3 mRNA ratio, oleate/glucose
1.35
ro , p / rd , p
PIP2 mRNA ratio, oleate/glucose
7.4
rg,a /rd ,a
rg,c / rd ,c
rg,o / rd ,o
rg,y / rd ,y
rg, p / rd , p
rg,c /rg,c,adr1 CTA1 mRNA ratio, non-induced, WT/(adr1)
rg, p / rg, p,adr1 PIP2 mRNA ratio, non-induced, WT/(adr1)
p d ,a
total Adr1p concentration, glucose
3.1
1.3
~50 molec/cell
p d ,c
total Cta1p concentration, glucose
623 molec/cell
p d ,o
total Oaf1p concentration, glucose
92.3 molec/cell
pd , y
total Oaf1p concentration, glucose
149 molec/cell
pd , p
total Pip2p concentration, glucose
~50 molec/cell
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pg,a
pg,c
pg,o
pg,y
pg, p
189 molec/cell
2,242 molec/cell
90.4 molec/cell
213 molec/cell
107 molec/cell
k d ,r ,a
total Adr1p concentration, glycerol
total Cta1p concentration, glycerol
total Oaf1p concentration, glycerol
total Oaf3p concentration, glycerol
total Pip2p concentration, glycerol
ADR1 mRNA max expression ratio (relative to
glucose)
CTA1 mRNA max expression ratio (relative to
glucose)
OAF1 mRNA max expression ratio (relative to
glucose)
OAF3 mRNA max expression ratio (relative to
glucose)
PIP2 mRNA max expression ratio
(relative to glucose)
ADR1 mRNA rate of degradation
k d , r ,c
CTA1 mRNA rate of degradation
0.0408 min-1
k d , r ,o
OAF1 mRNA rate of degradation
0.0462 min-1
k d ,r , y
OAF3 mRNA rate of degradation
0.0315 min-1
k d ,r , p
PIP2 mRNA rate of degradation
0.0315 min-1
k d , p ,a
Adr1p rate of degradation
0.0039 min-1
kd , p ,c
Cta1p rate of degradation
0.00048 min-1
kd , p , o
Oaf1p rate of degradation
0.0127 min-1
kd , p , y
Oaf3p rate of degradation
0.00481 min-1
kd , p , p
Pip2p rate of degradation
k i ,r ,a
ADR1 max rate of initiation of transcription
k i , r ,c
CTA1 max rate of initiation of transcription
k i , r ,o
OAF1 max rate of initiation of transcription
k i ,r , y
OAF3 max rate of initiation of transcription
k i ,r , p
PIP2 max rate of initiation of transcription
k i , p ,a
ADR1 rate of initiation of translation, per mRNA
0.0173 min-1
0.118 (molec/cell)
min-1
4.12 (molec/cell)
min-1
0.164 (molec/cell)
min-1
0.0403
(molec/cell) min-1
0.189 (molec/cell)
min-1
0.770 min-1
k i , p ,c
CTA1 rate of initiation of translation, per mRNA
0.865 min-1
ki , p , o
OAF1 rate of initiation of translation, per mRNA
1.95 min-1
ki , p , y
OAF3 rate of initiation of translation, per mRNA
1.79 min-1
rx ,a / rd ,a
rx ,c / rd ,c
rx ,o / rd ,o
rx , y / rd , y
rx , p / rd , p
15.0
97.1
5.9
3.2
20
0.0315 min-1
ki , p , p
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c
p
PIP2 rate of initiation of translation, per mRNA
Constitutive term in fractional activity of CTA1
Constitutive term in fractional activity of PIP2
N
Avogadro’s constant
A
Vc
Vn
Moa
cell cross-sectional area, on oleate
cell volume, on oleate
nuclear volume, on oleate
Conversion between molecules/cell and molar, for a
species that is localized to the nucleus
Molecular weight of oleic acid
H
Conversion between % and molar, for oleic acid
Oic
Concentration of intracellular oleic acid
Dissociation constant for Oaf1p activation by free
oleic acid
Cooperativity of Adr1p and Oaf1p-Pip2p binding to
DNA
Asymptotic ratio of intracellular to extracellular free
fatty acid
Minimum fractional activity of Adr1p protein
Equilibrium constant for Oaf1p-Pip2p binding to
promoter
Equilibrium constant for Adr1p binding to promoter
Activation constant for CTA1
Activation constant for PIP2
Equilibrium constant for Oaf3p binding to promoter
Michaelis constant for oleate transcriptional response
Dissociation constant for Oaf1p-Pip2p heterodimer
formation
Michaelis constant for activation of Adr1p
Q
K D ,o
q
r
a
Kh
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Ka
Ac
Ap
Ky
K M ,s
K D,h
K M ,a
2.88 min-1
0.0382
0.0819
6.022 × 1023
molec/mol
11.8 m2
3  10-14 L
2.1  10-15 L
7.9 × 10-10
M/(molec/cell)
282.46 g/mol
0.0354 M / (1 g /
100 mL)
0 – 4.2510-6 M
1.65 × 10-8 M
2.5
0.01
0.193
8.19 molec/cell
19.5 molec/cell
28
197.3
500 molec/cell
7.61 × 10-6 M
3.89 × 10-6 M
8.42 × 10-5 M