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Microbiology II
Topics: The introduction into microbial genetics built around the concept of information flow in
cells. Major topics include: Synthesis of nucleic acids and proteins in microbes;
Chromosomal replication and bacterial growth; Transcription and translation as coupled
stages of gene expression; Post-translational modifications of proteins; Regulation of
transcription in bacteria; Families of bacterial and animal viruses, their modes of
reproduction and pathogenicity; Bacteriophages and lateral gene transfer; Retroviruses
and reverse transcription; Other acellular genetic systems; Mutations and mechanisms
of genetic homeostasis in bacteria (DNA repair systems); Modes of gene transfer in
bacteria.
Lab:
Lab exercises include testing for antibiotic resistance, gene regulation, use of the
spectrophotometer, isolation and propagation of bacteriophage, mutation and DNA
repair, and gene transfer.
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