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Cells Use ‘Cellular’ Phones! Sivapriya Pavuluri In multicellular organisms, cells often do not live in isolation. They stick to other cells or to the noncellular components of their environment. Protein molecules on the cell surface make cells sticky. One of the most important groups of proteins that make cells sticky is integrin. An integrin is an integral membrane protein and is made up of two subunits, the subunit and the subunit. There are 24 types of integrins composed of one of 18 subunits and one of 8 subunits. Two main functions of integrins are attachment of cells together and transmission of signals from the extracellular matrix to the cell. Since integrins are useful for cell adhesion, growth, proliferation and differentiation, there might be a chance for cancer promoting factors to use integrins in order to avoid apoptosis and invade different parts of organs. Hence, it is useful to know how integrins work together with several growth factor receptors in a coordinated manner and different signaling mechanisms etc. During my project, I was able to perform a comparative analysis of the signals triggered by the 1subunit of integrin in 1A and 1B cells. I also cloned genes giving rise to smaller fragments of integrins f (with several combinations like ) and put all those genes into mouse cells. The idea behind iusing fragments rather than the whole integrins is to find the signaling patterns resulting from combination of different types of integrins. Every cell has to send information within its environment as well as outside. There are several proteins involved in carrying different messages like cell survival, cell death, cell division etc from cell to cell and with in cell to different molecules. Within the cell, integrins play a major role particularly in the process of cell division and cell adhesion. They send messages to the nucleus to perform these functions with the help of mediators called signaling proteins. There are different kinds of signaling proteins, one of which is called Mitogen-activated protein kinases. They are helpful in sending messages from integrins to the nucleus. In one of my experiment I was able to compare 1A and 1B cells and differentiate the signaling patterns of mitogen-activated protein kinases. Integrins are very important for the cell to adhere. Normal cells can adhere and cancer cells cannot adhere. Since integrins are major proteins responsible for adhesion, topics such as mechanism of cell signaling over the membrane by integrins, the cross talk between integrins and growth factor receptors through several signaling molecules needed to be found in a detailed manner. Knowledge on such topics will reveal how tumors can escape anchorage dependency growth and invade into several parts of the body. Degree project in Biology Examensarbete i biologi, 20 p, HT 2005 Biology Education Centre and Department of Medical Biochemistry and Microbiology, Uppsala University Supervisor: Staffan Johansson