Download 532321-AT-LC50-I-heltri

ROBUST STUDY SUMMARY - Inherent Toxicity
Item
Yes
No
Reference: Ewell, W.S., Gorsuch, J.W., Kringle, R.O. Robillard, K.A. and R.C. Spiegel. 1986. Simultaneous
Evaluation of the Acute Effects of Chemicals on Seven Aquatic Species. Environmental Toxicology and
Chemistry, 5: 831-840.
Test Substance (CAS # and name): 532-32-1 (benzoic acid, sodium salt)
*Chemical composition of the substance (including purity, by-products)
X
Persistence/stability of test substance in aquatic solution
X
Method
References
*OECD, EU, national, or other standard method?
Justification of the method/protocol if not a standard method was used
X
X
*GLP (Good Laboratory Practice)
n/a
n/a
Test organisms (specify common and Latin names): snail (Helisoma trivolvis)
Latin or both Latin and common names reported?
Life cycle age / stage of test organism
Sex
Length and weight of test organisms
Number of test organisms per replicate
Food type / feeding periods (acclimation/during test)
X
X
n/a
n/a
X
X
n/a
n/a
Test design / conditions
Test type – acute or chronic (specify, but do not assess this item): acute
Experiment type (laboratory or field) specified?
System type (static, semi-static, flow through)?
Negative or positive controls (specify)? Negative control only
Number of replicates (including controls) and concentrations
Exposure pathways (food, water, both)
Exposure duration
*Measured concentrations reported?
Exposure media conditions (temperature, pH, electrical conductivity, hardness, TOC,
DOC, DO, major cations and anions; other)
Was pH within 6-9 range? (do not assess this item)
Was temperature within 5-28 °C range? (do not assess this item)
Photoperiod and light intensity
Stock and test solution preparation
Use of emulgators / solubilizers (especially for poorly soluble / unstable substances)
Analytical monitoring intervals
Statistical methods used
X
X
X
X
X
X
X
X
X
X
X
X
n/a
n/a
X
X
Results
Toxicity values (LC50, EC50, or IC50 - specify, do not assess this item): LC50 = >100 mg/L (96h)
Other endpoints reported - BCF/BAF, LOEC/NOEC (specify, do not assess this item): No
*Was toxicity value below the chemical’s water solubility?
X
Other adverse effects (carcinogenicity, mutagenicity, etc. Do not assess this item)
Score: major items - 1/4; overall score – 16/21 (76%)
EC Reliability code: 3
Reliability category (high, satisfactory, low): low
Comments: Three major items answered “no”; overall score 76%. Nominal water concentrations. NO
X
determinable replicates. Simultaneous testing of multiple species (n=7) and multiple chemicals (n=27). Although
two of the 27 chemicals were compared to single species and single chemical exposures and were not found to be
significantly different, this can not be certain for all chemicals tested such that antagonistic, potentiative or
synergistic effects may occur for the other 25 chemicals. This method is not considered to be reasonable for
obtaining pivotal values for categorization purposes. Low confidence; reliability code = 3.