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QUICK REFERENCE PHLEBOTOMY GUIDE
For Laboratory Blood Collections
I. By tube type/color: Chemistry and Immunochemistry
A.Yellow With Gel Top Tubes:
 Basic/Comprehensive Panels
 Blood Alcohol
 BUN/Creatinine
 Electrolytes
 Glucose
 CPK
 HCG
 PSA
 Intact PTH
E. Green top with gel
CPKMB/Troponin
G. Lavender Top Tube:
Hemoglobin A1c/Glycohcg
RBC Folate
B. Plain Red Top:
Thyroid Tests
* Therapeutic Drug Levels:
Dilantin, Digoxin,
Phenobarb., Tegetol
Vanco., Tobramycin
C. Large Purple Top Tube:
*BNP
D. Gray Top Tubes:
*Lactate (on ice)
F. Green Top Tube:
*Ammonia (on ice)
II. By tube type/color: Hematology
A. Lavender
B. Blue*must fill tubes
 CBC
*Prothrombin time (PT)
 CBC with Diff
*PTT
 Platelet Count
*Unfractionated Heparin
 Hemoglobin/Hematocrit
*Low Molecular Weight
 Retic
Heparin (LMWH)
* Fibrinogen
 Sed Rate
* D-Dimer
 Pediatric Blood Bank
PFA call Hem @28147
*Bleeding Time Call Hem
PLAVIX (special tube)
Call Hemo 28147
III. Blood Bank/Transfusion Services
Pink Top , 6ml EDTA tube
Optional tubes, both lavender and red
IV. Microbiology/Blood Cultures
 Blue and Purple bottles is a set: 5-10mls/bottle
 Yellow, pediatric/low volume: 1-4mls
 Blue, fungal blood culture: 5-10mls/bottle
 Black, AFB blood culture, call Micro 2-8152
V. Laboratory Collection Guide in Alpha Order
Must identify both patient and specimen following red rules
Test
Ammonia
Basic/Comprehensive
Panels
Blood bank testing
Tube
Type
Green
Yellow
Special Notes
Transport on ice
Pink 6ml Collector and Witness
required for collection
Blood Cultures,routine Blue/
5-10 mls/bottle
Purple
Must collect
aseptically
Blood Cultures, pedi
Yellow
1-4 mls /bottle
B-NP
Purple
6ml tube
CBC with Diff
Lavender
CBC
Lavender
CPK
Yellow
CPKMB/Troponin
Green
with gel
D-Dimer
Blue
Tube must be full
Electrolytes
Yellow
Fibrinogen
Blue
Tube must be full
Glucose
Yellow
HCG
Yellow
Hemoglobin/Hematocrit Lavender
Hemoglobin A1c
Lavender
Heparin,low molecular Blue
Tube must be full
weight (LMWH)
Heparin, Unfractionated Blue
Tube must be full
Intact PTH
Yellow
Dept.
Chemistry
Chemistry
Blood Bank
Microbiology
*call for afb
to 2-8152
microbiology
Chemistry
Hematology
Hematology
Chemistry
Chemistry
Hematology
Chemistry
Hematology
Chemistry
Chemistry
Hematolgy
Chemistry
Hematology
Hematology
Chemistry
Lactate
Platelet Count
Plavix
Prothrombin Time (PT)
PSA
PTT
Retic
Sed Rate/ESR
Thyroid Tests
Gray
Lavender
Special
tube
Blue
Yellow
Blue
Lavender
Lavender
or Black
Red
Transport on ice
Call hematology
28147
Tube must be full
Tube must be full
Tube must be full
Chemistry
Hematology
Hematology
Hematology
Chemistry
Hematology
Hematology
Hematology
Chemistry
Bleeding Time and PFA, Blue Top, call Hematology at 2-8147
Order of draw for test tubes
Vacutainer tube methode
White (discard)
Blue
Gold or Red
Lavender or Pink
Syringe and safety transfer device
Blue
Lavender or Pink
Gold or Red
Blood cultures are ALWAYS drawn first
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