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GENE EXPRESSION QUESTIONS 1. What is the "one-gene one-polypeptide" hypothesis? 2. A) Why are there three letters (bases) in the "words" of the genetic code? B) Why can't two letter "words" be used in the genetic code? 3. Is it true that there is redundancy but not ambiguity in the genetic code? 4. What are the two main steps in protein synthesis? 5. What is mRNA and how is it made? 6. What is RNA polymerase and in what direction does it work? 7. What are "promoters"? 8. Diagram a tRNA; indicate the anticodon and the site of amino acid attachment. 9. Describe the structure of the ribosome. How does a prokaryotic ribosome differ from a eukaryotic ribosome? 1 10. What are the three steps in translation and what happens in each step? 11. What polypeptide would result from the following single strand of DNA: ATCCGTAAGAATGCGCAT 12. What is a mutation? What causes mutations? 13. Describe substitution mutations and tell how the affect protein synthesis. 14. Describe frameshift mutations and tell how they affect protein synthesis. 2