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Date of Document: June 8, 2017
CURRICULUM VITAE
David N. Frick PhD
Associate Professor
Department of Chimistry & Biochemistry
OFFICE ADDRESS:
3210 N. Cramer St
Milwaukee, WI 53211
BIBLIOGRAPHY
Refereed Journal Publications/Original Papers
1. Abeygunawardana C, Weber DJ, Gittis AG, Frick DN, Lin J, Miller AF, Bessman MJ, Mildvan AS. Solution
structure of the MutT enzyme, a nucleoside triphosphate pyrophosphohydrolase. Biochemistry. 1995 Nov
21;34(46):14997-5005.
2. Frick DN, Townsend BD, Bessman MJ. A novel GDP-mannose mannosyl hydrolase shares homology with the MutT
family of enzymes. J Biol Chem. 1995 Oct 13;270(41):24086-91.
3. Frick DN, Weber DJ, Abeygunawardana C, Gittis AG, Bessman MJ, Mildvan AS. NMR studies of the
conformations and location of nucleotides bound to the Escherichia coli MutT enzyme. Biochemistry. 1995 Apr
25;34(16):5577-86.
4. Frick DN, Bessman MJ. Cloning, purification, and properties of a novel NADH pyrophosphatase. Evidence for a
nucleotide pyrophosphatase catalytic domain in MutT-like enzymes. J Biol Chem. 1995 Jan 27;270(4):1529-34.
5. Abeygunawardana C, Weber DJ, Frick DN, Bessman MJ, Mildvan AS. Sequence-specific assignments of the
backbone 1H, 13C, and 15N resonances of the MutT enzyme by heteronuclear multidimensional NMR. Biochemistry.
1993 Dec 07;32(48):13071-80.
6. Frick DN, Weber DJ, Gillespie JR, Bessman MJ, Mildvan AS. Dual divalent cation requirement of the MutT
dGTPase. Kinetic and magnetic resonance studies of the metal and substrate complexes. J Biol Chem. 1994 Jan
21;269(3):1794-803.
7. Lin J, Abeygunawardana C, Frick DN, Bessman MJ, Mildvan AS. The role of Glu 57 in the mechanism of the
Escherichia coli MutT enzyme by mutagenesis and heteronuclear NMR. Biochemistry. 1996 May 28;35(21):6715-26.
8. O'Handley SF, Frick DN, Bullions LC, Mildvan AS, Bessman MJ. Escherichia coli orf17 codes for a nucleoside
triphosphate pyrophosphohydrolase member of the MutT family of proteins. Cloning, purification, and characterization
of the enzyme. J Biol Chem. 1996 Oct 04;271(40):24649-54.
9. Bessman MJ, Frick DN, O'Handley SF. The MutT proteins or "Nudix" hydrolases, a family of versatile, widely
distributed, "housecleaning" enzymes. J Biol Chem. 1996 Oct 11;271(41):25059-62.
10. Lin J, Abeygunawardana C, Frick DN, Bessman MJ, Mildvan AS. Solution structure of the quaternary MutT-M2+AMPCPP-M2+ complex and mechanism of its pyrophosphohydrolase action. Biochemistry. 1997 Feb 11;36(6):1199211.
11. O'Handley SF, Frick DN, Dunn CA, Bessman MJ. Orf186 represents a new member of the Nudix hydrolases,
active on adenosine(5')triphospho(5')adenosine, ADP-ribose, and NADH. J Biol Chem. 1998 Feb 06;273(6):3192-7.
12. Frick DN, Baradaran K, Richardson CC. An N-terminal fragment of the gene 4 helicase/primase of bacteriophage
T7 retains primase activity in the absence of helicase activity. Proc Natl Acad Sci U S A. 1998 Jul 07;95(14):7957-62.
PMCID: PMC20911
13. Dunn CA, O'Handley SF, Frick DN, Bessman MJ. Studies on the ADP-ribose pyrophosphatase subfamily of the
nudix hydrolases and tentative identification of trgB, a gene associated with tellurite resistance. J Biol Chem. 1999 Nov
05;274(45):32318-24.
14. Frick DN, Richardson CC. Interaction of bacteriophage T7 gene 4 primase with its template recognition site. J Biol
Chem. 1999 Dec 10;274(50):35889-98.
15. Frick DN, Kumar S, Richardson CC. Interaction of ribonucleoside triphosphates with the gene 4 primase of
bacteriophage T7. J Biol Chem. 1999 Dec 10;274(50):35899-907.
16. Tseng TY, Frick DN, Richardson CC. Characterization of a novel DNA primase from the Salmonella typhimurium
bacteriophage SP6. Biochemistry. 2000 Feb 22;39(7):1643-54.
17. Kato M, Frick DN, Lee J, Tabor S, Richardson CC, Ellenberger T. A complex of the bacteriophage T7 primasehelicase and DNA polymerase directs primer utilization. J Biol Chem. 2001 Jun 15;276(24):21809-20.
18. Frick DN, Richardson CC. DNA primases. Annu Rev Biochem. 2001;70:39-80.
19. Lam AM, Keeney D, Eckert PQ, Frick DN. Hepatitis C virus NS3 ATPases/helicases from different genotypes
exhibit variations in enzymatic properties. J Virol. 2003 Apr;77(7):3950-61. PMCID: PMC150621
20. Lam AM, Keeney D, Frick DN. Two novel conserved motifs in the hepatitis C virus NS3 protein critical for
helicase action. J Biol Chem. 2003 Nov 07;278(45):44514-24. PMCID: PMC3571693
21. Frick DN. Helicases as antiviral drug targets. Drug News Perspect. 2003 Jul-Aug;16(6):355-62. PMCID:
PMC3571683
22. Frick DN, Rypma RS, Lam AM, Gu B. The nonstructural protein 3 protease/helicase requires an intact protease
domain to unwind duplex RNA efficiently. J Biol Chem. 2004 Jan 09;279(2):1269-80. PMCID: PMC3571687
23. Lam AM, Rypma RS, Frick DN. Enhanced nucleic acid binding to ATP-bound hepatitis C virus NS3 helicase at
low pH activates RNA unwinding. Nucleic Acids Res. 2004;32(13):4060-70. PMCID: PMC506820
24. Frick DN, Rypma RS, Lam AM, Frenz CM. Electrostatic analysis of the hepatitis C virus NS3 helicase reveals both
active and allosteric site locations. Nucleic Acids Res. 2004;32(18):5519-28. PMCID: PMC524300
25. Lam AM, Frick DN. Hepatitis C virus subgenomic replicon requires an active NS3 RNA helicase. J Virol. 2006
Jan;80(1):404-11. PMCID: PMC1317551
26. Frick DN, Lam AM. Understanding helicases as a means of virus control. Curr Pharm Des. 2006;12(11):1315-38.
PMCID: PMC3571686
27. Frick DN. Step-by-step progress toward understanding the hepatitis C virus RNA helicase. Hepatology. 2006
Jun;43(6):1392-5. PMCID: PMC3571624
28. Frick DN, Banik S, Rypma RS. Role of divalent metal cations in ATP hydrolysis catalyzed by the hepatitis C virus
NS3 helicase: magnesium provides a bridge for ATP to fuel unwinding. J Mol Biol. 2007 Jan 26;365(4):1017-32.
PMCID: PMC1829317
29. Frick DN. The hepatitis C virus NS3 protein: a model RNA helicase and potential drug target. Curr Issues Mol
Biol. 2007 Jan;9(1):1-20. PMCID: PMC3571657
30. Neumann-Haefelin C, Frick DN, Wang JJ, Pybus OG, Salloum S, Narula GS, Eckart A, Biezynski A, Eiermann T,
Klenerman P, Viazov S, Roggendorf M, Thimme R, Reiser M, Timm J. Analysis of the evolutionary forces in an
immunodominant CD8 epitope in hepatitis C virus at a population level. J Virol. 2008 Apr;82(7):3438-51. PMCID:
PMC2268453
31. Heck JA, Lam AM, Narayanan N, Frick DN. Effects of mutagenic and chain-terminating nucleotide analogs on
enzymes isolated from hepatitis C virus strains of various genotypes. Antimicrob Agents Chemother. 2008
Jun;52(6):1901-11. PMCID: PMC2415805
32. Belon CA, Frick DN. Monitoring helicase activity with molecular beacons. Biotechniques. 2008 Oct;45(4):433-40,
442. PMCID: PMC2575809
33. Meng X, Zhou Y, Zhang S, Lee EY, Frick DN, Lee MY. DNA damage alters DNA polymerase delta to a form that
exhibits increased discrimination against modified template bases and mismatched primers. Nucleic Acids Res. 2009
Feb;37(2):647-57. PMCID: PMC2632934
34. Heck JA, Meng X, Frick DN. Cyclophilin B stimulates RNA synthesis by the HCV RNA dependent RNA
polymerase. Biochem Pharmacol. 2009 Apr 01;77(7):1173-80. PMCID: PMC2656422
35. Belon CA, Frick DN. Fuel specificity of the hepatitis C virus NS3 helicase. J Mol Biol. 2009 May 15;388(4):85164. PMCID: PMC2692962
36. Belon CA, High YD, Lin TI, Pauwels F, Frick DN. Mechanism and specificity of a symmetrical
benzimidazolephenylcarboxamide helicase inhibitor. Biochemistry. 2010 Mar 09;49(9):1822-32. PMCID:
PMC2832472
37. Belon CA, Frick DN. Helicase inhibitors as specifically targeted antiviral therapy for hepatitis C. Future Virol.
2009 May 01;4(3):277-293. PMCID: PMC2714653
38. Frick DN, Ginzburg O, Lam AM. A method to simultaneously monitor hepatitis C virus NS3 helicase and protease
activities. Methods Mol Biol. 2010;587:223-33. PMCID: PMC2838209
39. Meng X, Zhou Y, Lee EY, Lee MY, Frick DN. The p12 subunit of human polymerase delta modulates the rate and
fidelity of DNA synthesis. Biochemistry. 2010 May 04;49(17):3545-54. PMCID: PMC2860660
40. Lemon SM, McKeating JA, Pietschmann T, Frick DN, Glenn JS, Tellinghuisen TL, Symons J, Furman PA.
Development of novel therapies for hepatitis C. Antiviral Res. 2010 Apr;86(1):79-92.
41. Lam AM, Murakami E, Espiritu C, Steuer HM, Niu C, Keilman M, Bao H, Zennou V, Bourne N, Julander JG,
Morrey JD, Smee DF, Frick DN, Heck JA, Wang P, Nagarathnam D, Ross BS, Sofia MJ, Otto MJ, Furman PA. PSI7851, a pronucleotide of beta-D-2'-deoxy-2'-fluoro-2'-C-methyluridine monophosphate, is a potent and pan-genotype
inhibitor of hepatitis C virus replication. Antimicrob Agents Chemother. 2010 Aug;54(8):3187-96. PMCID:
PMC2916354
42. Mousseau G, Kota S, Takahashi V, Frick DN, Strosberg AD. Dimerization-driven interaction of hepatitis C virus
core protein with NS3 helicase. J Gen Virol. 2011 Jan;92(Pt 1):101-11. PMCID: PMC3052529
43. Peng LF, Schaefer EA, Maloof N, Skaff A, Berical A, Belon CA, Heck JA, Lin W, Frick DN, Allen TM, Miziorko
HM, Schreiber SL, Chung RT. Ceestatin, a novel small molecule inhibitor of hepatitis C virus replication, inhibits 3hydroxy-3-methylglutaryl-coenzyme A synthase. J Infect Dis. 2011 Aug 15;204(4):609-16. PMCID: PMC3144167
44. Li K, Frankowski KJ, Belon CA, Neuenswander B, Ndjomou J, Hanson AM, Shanahan MA, Schoenen FJ, Blagg
BS, Aubé J, Frick DN. Optimization of potent hepatitis C virus NS3 helicase inhibitors isolated from the yellow dyes
thioflavine S and primuline. J Med Chem. 2012 Apr 12;55(7):3319-30. PMCID: PMC3476947
45. Mastrangelo E, Pezzullo M, De Burghgraeve T, Kaptein S, Pastorino B, Dallmeier K, de Lamballerie X, Neyts J,
Hanson AM, Frick DN, Bolognesi M, Milani M. Ivermectin is a potent inhibitor of flavivirus replication specifically
targeting NS3 helicase activity: new prospects for an old drug. J Antimicrob Chemother. 2012 Aug;67(8):1884-94.
PMCID: PMC3888155
46. Hanson AM, Hernandez JJ, Shadrick WR, Frick DN. Identification and analysis of inhibitors targeting the hepatitis
C virus NS3 helicase. Methods Enzymol. 2012;511:463-83. PMCID: PMC3571682
47. Mukherjee S, Hanson AM, Shadrick WR, Ndjomou J, Sweeney NL, Hernandez JJ, Bartczak D, Li K, Frankowski
KJ, Heck JA, Arnold LA, Schoenen FJ, Frick DN. Identification and analysis of hepatitis C virus NS3 helicase
inhibitors using nucleic acid binding assays. Nucleic Acids Res. 2012 Sep 01;40(17):8607-21. PMCID: PMC3458564
48. Ndjomou J, Kolli R, Mukherjee S, Shadrick WR, Hanson AM, Sweeney NL, Bartczak D, Li K, Frankowski KJ,
Schoenen FJ, Frick DN. Fluorescent primuline derivatives inhibit hepatitis C virus NS3-catalyzed RNA unwinding,
peptide hydrolysis and viral replicase formation. Antiviral Res. 2012 Nov;96(2):245-55. PMCID: PMC3571690
49. Shadrick WR, Ndjomou J, Kolli R, Mukherjee S, Hanson AM, Frick DN. Discovering new medicines targeting
helicases: challenges and recent progress. J Biomol Screen. 2013 Aug;18(7):761-81. PMCID: PMC4427233
50. Mannan MA, Shadrick WR, Biener G, Shin BS, Anshu A, Raicu V, Frick DN, Dey M. An ire1-phk1 chimera
reveals a dispensable role of autokinase activity in endoplasmic reticulum stress response. J Mol Biol. 2013 Jun
26;425(12):2083-99.
51. Sweeney NL, Shadrick WR, Mukherjee S, Li K, Frankowski KJ, Schoenen FJ, Frick DN. Primuline derivatives that
mimic RNA to stimulate hepatitis C virus NS3 helicase-catalyzed ATP hydrolysis. J Biol Chem. 2013 Jul
05;288(27):19949-57. PMCID: PMC3707695
52. Shadrick WR, Mukherjee S, Hanson AM, Sweeney NL, Frick DN. Aurintricarboxylic acid modulates the affinity of
hepatitis C virus NS3 helicase for both nucleic acid and ATP. Biochemistry. 2013 Sep 10;52(36):6151-9. PMCID:
PMC4272346
53. Aydin C, Mukherjee S, Hanson AM, Frick DN, Schiffer CA. The interdomain interface in bifunctional enzyme
protein 3/4A (NS3/4A) regulates protease and helicase activities. Protein Sci. 2013 Dec;22(12):1786-98. PMCID:
PMC3843632
54. Abeygunawardana C, Weber DJ, Gittis AG, Frick DN, Lin J, Miller AF, Bessman MJ, Mildvan AS Solution
structure of the MutT enzyme, a nucleoside triphosphate pyrophosphohydrolase. Biochemistry. 1995 Nov
21;34(46):14997-5005
55. Frick DN, Townsend BD, Bessman MJ A novel GDP-mannose mannosyl hydrolase shares homology with the
MutT family of enzymes. J Biol Chem. 1995 Oct 13;270(41):24086-91
56. Frick DN, Weber DJ, Abeygunawardana C, Gittis AG, Bessman MJ, Mildvan AS NMR studies of the
conformations and location of nucleotides bound to the Escherichia coli MutT enzyme. Biochemistry. 1995 Apr
25;34(16):5577-86
57. Frick DN, Bessman MJ Cloning, purification, and properties of a novel NADH pyrophosphatase. Evidence for a
nucleotide pyrophosphatase catalytic domain in MutT-like enzymes. J Biol Chem. 1995 Jan 27;270(4):1529-34
58. Abeygunawardana C, Weber DJ, Frick DN, Bessman MJ, Mildvan AS Sequence-specific assignments of the
backbone 1H, 13C, and 15N resonances of the MutT enzyme by heteronuclear multidimensional NMR. Biochemistry.
1993 Dec 7;32(48):13071-80
59. Frick DN, Weber DJ, Gillespie JR, Bessman MJ, Mildvan AS Dual divalent cation requirement of the MutT
dGTPase. Kinetic and magnetic resonance studies of the metal and substrate complexes. J Biol Chem. 1994 Jan
21;269(3):1794-803
60. Lin J, Abeygunawardana C, Frick DN, Bessman MJ, Mildvan AS The role of Glu 57 in the mechanism of the
Escherichia coli MutT enzyme by mutagenesis and heteronuclear NMR. Biochemistry. 1996 May 28;35(21):6715-26
61. O'Handley SF, Frick DN, Bullions LC, Mildvan AS, Bessman MJ Escherichia coli orf17 codes for a nucleoside
triphosphate pyrophosphohydrolase member of the MutT family of proteins. Cloning, purification, and characterization
of the enzyme. J Biol Chem. 1996 Oct 4;271(40):24649-54
62. Bessman MJ, Frick DN, O'Handley SF The MutT proteins or "Nudix" hydrolases, a family of versatile, widely
distributed, "housecleaning" enzymes. J Biol Chem. 1996 Oct 11;271(41):25059-62
63. Lin J, Abeygunawardana C, Frick DN, Bessman MJ, Mildvan AS Solution structure of the quaternary MutT-M2+AMPCPP-M2+ complex and mechanism of its pyrophosphohydrolase action. Biochemistry. 1997 Feb 11;36(6):1199211
64. O'Handley SF, Frick DN, Dunn CA, Bessman MJ Orf186 represents a new member of the Nudix hydrolases, active
on adenosine(5')triphospho(5')adenosine, ADP-ribose, and NADH. J Biol Chem. 1998 Feb 6;273(6):3192-7
65. Frick DN, Baradaran K, Richardson CC An N-terminal fragment of the gene 4 helicase/primase of bacteriophage
T7 retains primase activity in the absence of helicase activity. Proc Natl Acad Sci U S A. 1998 Jul 7;95(14):7957-62
66. Dunn CA, O'Handley SF, Frick DN, Bessman MJ Studies on the ADP-ribose pyrophosphatase subfamily of the
nudix hydrolases and tentative identification of trgB, a gene associated with tellurite resistance. J Biol Chem. 1999 Nov
5;274(45):32318-24
67. Frick DN, Richardson CC Interaction of bacteriophage T7 gene 4 primase with its template recognition site. J Biol
Chem. 1999 Dec 10;274(50):35889-98
68. Frick DN, Kumar S, Richardson CC Interaction of ribonucleoside triphosphates with the gene 4 primase of
bacteriophage T7. J Biol Chem. 1999 Dec 10;274(50):35899-907
69. Tseng TY, Frick DN, Richardson CC Characterization of a novel DNA primase from the Salmonella typhimurium
bacteriophage SP6. Biochemistry. 2000 Feb 22;39(7):1643-54
70. Kato M, Frick DN, Lee J, Tabor S, Richardson CC, Ellenberger T A complex of the bacteriophage T7 primasehelicase and DNA polymerase directs primer utilization. J Biol Chem. 2001 Jun 15;276(24):21809-20
71. Frick DN, Richardson CC DNA primases. Annu Rev Biochem. 2001;70:39-80
72. Lam AM, Keeney D, Eckert PQ, Frick DN Hepatitis C virus NS3 ATPases/helicases from different genotypes
exhibit variations in enzymatic properties. J Virol. 2003 Apr;77(7):3950-61
73. Lam AM, Keeney D, Frick DN Two novel conserved motifs in the hepatitis C virus NS3 protein critical for
helicase action. J Biol Chem. 2003 Nov 7;278(45):44514-24
74. Frick DN Helicases as antiviral drug targets. Drug News Perspect. 2003 Jul-Aug;16(6):355-62
75. Frick DN, Rypma RS, Lam AM, Gu B The nonstructural protein 3 protease/helicase requires an intact protease
domain to unwind duplex RNA efficiently. J Biol Chem. 2004 Jan 9;279(2):1269-80
76. Lam AM, Rypma RS, Frick DN Enhanced nucleic acid binding to ATP-bound hepatitis C virus NS3 helicase at low
pH activates RNA unwinding. Nucleic Acids Res. 2004;32(13):4060-70
77. Frick DN, Rypma RS, Lam AM, Frenz CM Electrostatic analysis of the hepatitis C virus NS3 helicase reveals both
active and allosteric site locations. Nucleic Acids Res. 2004;32(18):5519-28
78. Lam AM, Frick DN Hepatitis C virus subgenomic replicon requires an active NS3 RNA helicase. J Virol. 2006
Jan;80(1):404-11
79. Frick DN, Lam AM Understanding helicases as a means of virus control. Curr Pharm Des. 2006;12(11):1315-38
80. Frick DN Step-by-step progress toward understanding the hepatitis C virus RNA helicase. Hepatology. 2006
Jun;43(6):1392-5
81. Frick DN, Banik S, Rypma RS Role of divalent metal cations in ATP hydrolysis catalyzed by the hepatitis C virus
NS3 helicase: magnesium provides a bridge for ATP to fuel unwinding. J Mol Biol. 2007 Jan 26;365(4):1017-32
82. Frick DN The hepatitis C virus NS3 protein: a model RNA helicase and potential drug target. Curr Issues Mol Biol.
2007 Jan;9(1):1-20
83. Neumann-Haefelin C, Frick DN, Wang JJ, Pybus OG, Salloum S, Narula GS, Eckart A, Biezynski A, Eiermann T,
Klenerman P, Viazov S, Roggendorf M, Thimme R, Reiser M, Timm J Analysis of the evolutionary forces in an
immunodominant CD8 epitope in hepatitis C virus at a population level. J Virol. 2008 Apr;82(7):3438-51
84. Heck JA, Lam AM, Narayanan N, Frick DN Effects of mutagenic and chain-terminating nucleotide analogs on
enzymes isolated from hepatitis C virus strains of various genotypes. Antimicrob Agents Chemother. 2008
Jun;52(6):1901-11
85. Belon CA, Frick DN Monitoring helicase activity with molecular beacons. Biotechniques. 2008 Oct;45(4):433-40,
442
86. Meng X, Zhou Y, Zhang S, Lee EY, Frick DN, Lee MY DNA damage alters DNA polymerase delta to a form that
exhibits increased discrimination against modified template bases and mismatched primers. Nucleic Acids Res. 2009
Feb;37(2):647-57
87. Heck JA, Meng X, Frick DN Cyclophilin B stimulates RNA synthesis by the HCV RNA dependent RNA
polymerase. Biochem Pharmacol. 2009 Apr 1;77(7):1173-80
88. Belon CA, Frick DN Fuel specificity of the hepatitis C virus NS3 helicase. J Mol Biol. 2009 May 15;388(4):851-64
89. Belon CA, High YD, Lin TI, Pauwels F, Frick DN Mechanism and specificity of a symmetrical
benzimidazolephenylcarboxamide helicase inhibitor. Biochemistry. 2010 Mar 9;49(9):1822-32
90. Belon CA, Frick DN Helicase inhibitors as specifically targeted antiviral therapy for hepatitis C. Future Virol. 2009
May 1;4(3):277-293
91. Frick DN, Ginzburg O, Lam AM A method to simultaneously monitor hepatitis C virus NS3 helicase and protease
activities. Methods Mol Biol. 2010;587:223-33
92. Meng X, Zhou Y, Lee EY, Lee MY, Frick DN The p12 subunit of human polymerase delta modulates the rate and
fidelity of DNA synthesis. Biochemistry. 2010 May 4;49(17):3545-54
93. Lemon SM, McKeating JA, Pietschmann T, Frick DN, Glenn JS, Tellinghuisen TL, Symons J, Furman PA
Development of novel therapies for hepatitis C. Antiviral Res. 2010 Apr;86(1):79-92
94. Lam AM, Murakami E, Espiritu C, Steuer HM, Niu C, Keilman M, Bao H, Zennou V, Bourne N, Julander JG,
Morrey JD, Smee DF, Frick DN, Heck JA, Wang P, Nagarathnam D, Ross BS, Sofia MJ, Otto MJ, Furman PA PSI7851, a pronucleotide of beta-D-2'-deoxy-2'-fluoro-2'-C-methyluridine monophosphate, is a potent and pan-genotype
inhibitor of hepatitis C virus replication. Antimicrob Agents Chemother. 2010 Aug;54(8):3187-96
95. Mousseau G, Kota S, Takahashi V, Frick DN, Strosberg AD Dimerization-driven interaction of hepatitis C virus
core protein with NS3 helicase. J Gen Virol. 2011 Jan;92(Pt 1):101-11
96. Peng LF, Schaefer EA, Maloof N, Skaff A, Berical A, Belon CA, Heck JA, Lin W, Frick DN, Allen TM, Miziorko
HM, Schreiber SL, Chung RT Ceestatin, a novel small molecule inhibitor of hepatitis C virus replication, inhibits 3hydroxy-3-methylglutaryl-coenzyme A synthase. J Infect Dis. 2011 Aug 15;204(4):609-16
97. Li K, Frankowski KJ, Belon CA, Neuenswander B, Ndjomou J, Hanson AM, Shanahan MA, Schoenen FJ, Blagg
BS, Aubé J, Frick DN Optimization of potent hepatitis C virus NS3 helicase inhibitors isolated from the yellow dyes
thioflavine S and primuline. J Med Chem. 2012 Apr 12;55(7):3319-30
98. Mastrangelo E, Pezzullo M, De Burghgraeve T, Kaptein S, Pastorino B, Dallmeier K, de Lamballerie X, Neyts J,
Hanson AM, Frick DN, Bolognesi M, Milani M Ivermectin is a potent inhibitor of flavivirus replication specifically
targeting NS3 helicase activity: new prospects for an old drug. J Antimicrob Chemother. 2012 Aug;67(8):1884-94
99. Hanson AM, Hernandez JJ, Shadrick WR, Frick DN Identification and analysis of inhibitors targeting the hepatitis
C virus NS3 helicase. Methods Enzymol. 2012;511:463-83
100. Mukherjee S, Hanson AM, Shadrick WR, Ndjomou J, Sweeney NL, Hernandez JJ, Bartczak D, Li K, Frankowski
KJ, Heck JA, Arnold LA, Schoenen FJ, Frick DN Identification and analysis of hepatitis C virus NS3 helicase
inhibitors using nucleic acid binding assays. Nucleic Acids Res. 2012 Sep 1;40(17):8607-21
101. Ndjomou J, Kolli R, Mukherjee S, Shadrick WR, Hanson AM, Sweeney NL, Bartczak D, Li K, Frankowski KJ,
Schoenen FJ, Frick DN Fluorescent primuline derivatives inhibit hepatitis C virus NS3-catalyzed RNA unwinding,
peptide hydrolysis and viral replicase formation. Antiviral Res. 2012 Nov;96(2):245-55
102. Shadrick WR, Ndjomou J, Kolli R, Mukherjee S, Hanson AM, Frick DN Discovering new medicines targeting
helicases: challenges and recent progress. J Biomol Screen. 2013 Aug;18(7):761-81
103. Mannan MA, Shadrick WR, Biener G, Shin BS, Anshu A, Raicu V, Frick DN, Dey M An ire1-phk1 chimera
reveals a dispensable role of autokinase activity in endoplasmic reticulum stress response. J Mol Biol. 2013 Jun
26;425(12):2083-99
104. Sweeney NL, Shadrick WR, Mukherjee S, Li K, Frankowski KJ, Schoenen FJ, Frick DN Primuline derivatives
that mimic RNA to stimulate hepatitis C virus NS3 helicase-catalyzed ATP hydrolysis. J Biol Chem. 2013 Jul
5;288(27):19949-57
105. Shadrick WR, Mukherjee S, Hanson AM, Sweeney NL, Frick DN Aurintricarboxylic Acid Modulates the Affinity
of Hepatitis C Virus NS3 Helicase for Both Nucleic Acid and ATP. Biochemistry. 2013 Aug 26:
106. Aydin C, Mukherjee S, Hanson AM, Frick DN, Schiffer CA The interdomain interface in bifunctional enzyme
protein 3/4A (NS3/4A) regulates protease and helicase activities. Protein Sci. 2013 Dec;22(12):1786-98
107. Mukherjee S, Weiner WS, Schroeder CE, Simpson DS, Hanson AM, Sweeney NL, Marvin RK, Ndjomou J, Kolli
R, Isailovic D, Schoenen FJ, Frick DN. Ebselen inhibits hepatitis C virus NS3 helicase binding to nucleic acid and
prevents viral replication. ACS Chem Biol. 2014 Oct 17;9(10):2393-403. PMCID: PMC4201343
108. Andreev IA, Manvar D, Barreca ML, Belov DS, Basu A, Sweeney NL, Ratmanova NK, Lukyanenko ER,
Manfroni G, Cecchetti V, Frick DN, Altieri A, Kaushik-Basu N, Kurkin AV. Discovery of the 2-phenyl-4,5,6,7Tetrahydro-1H-indole as a novel anti-hepatitis C virus targeting scaffold. Eur J Med Chem. 2015;96:250-8.
109. Sweeney NL, Hanson AM, Mukherjee S, Ndjomou J, Geiss BJ, Steel JJ, Frankowski KJ, Li K, Schoenen FJ, Frick
DN. Benzothiazole and Pyrrolone Flavivirus Inhibitors Targeting the Viral Helicase. ACS Infect Dis. 2015 Mar
13;1(3):140-148. PMCID: PMC4444071
110. Ndjomou J, Corby MJ, Sweeney NL, Hanson AM, Aydin C, Ali A, Schiffer CA, Li K, Frankowski KJ, Schoenen
FJ, Frick DN. Simultaneously Targeting the NS3 Protease and Helicase Activities for More Effective Hepatitis C Virus
Therapy. ACS Chem Biol. 2015 Aug 21;10(8):1887-96. PMCID: PMC4546510
111. Provazzi PJ, Mukherjee S, Hanson AM, Nogueira ML, Carneiro BM, Frick DN, Rahal P. Analysis of the
Enzymatic Activity of an NS3 Helicase Genotype 3a Variant Sequence Obtained from a Relapse Patient. PLoS One.
2015;10(12):e0144638. PMCID: PMC4684341
112. Kaushik-Basu N, Ratmanova NK, Manvar D, Belov DS, Cevik O, Basu A, Yerukhimovich MM, Lukyanenko ER,
Andreev IA, Belov GM, Manfroni G, Cecchetti V, Frick DN, Kurkin AV, Altieri A, Barreca ML. Bicyclic
octahydrocyclohepta[b]pyrrol-4(1H)one derivatives as novel selective anti-hepatitis C virus agents. Eur J Med Chem.
2016 Oct 21;122:319-25.
113. Bassetto M, Leyssen P, Neyts J, Yerukhimovich MM, Frick DN, Brancale A. Computer-aided identification,
synthesis and evaluation of substituted thienopyrimidines as novel inhibitors of HCV replication. Eur J Med Chem.
2016 Nov 10;123:31-47.
114. Bassetto M, Ferla S, Leyssen P, Neyts J, Yerukhimovich MM, Frick DN, O'Donnell R, Brancale A. Novel
symmetrical phenylenediamines as potential anti-hepatitis C virus agents. Antivir Chem Chemother. 2016 Nov 04.
115. Bassetto M, Leyssen P, Neyts J, Yerukhimovich MM, Frick DN, Courtney-Smith M, Brancale A. In silico
identification, design and synthesis of novel piperazine-based antiviral agents targeting the hepatitis C virus helicase.
Eur J Med Chem. 2017 Jan 05;125:1115-1131.
116. Bassetto M, Leyssen P, Neyts J, Yerukhimovich MM, Frick DN, Brancale A. Shape-based virtual screening,
synthesis and evaluation of novel pyrrolone derivatives as antiviral agents against HCV. Bioorg Med Chem Lett. 2017
Feb 15;27(4):936-940.
117. Corby MJ, Stoneman MR, Biener G, Paprocki JD, Kolli R, Raicu V, Frick DN. Quantitative Micro-Spectroscopic
Imaging Reveals Viral and Cellular RNA Helicase Interactions in Live Cells. J Biol Chem. 2017 May 08.