Download Pre-lab Materials - Harvard Life Science Outreach Program

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Transcript 
• Summer 2008 Workshop
• in Biology and Multimedia
• for High School Teachers
Life Sciences-HHMI Outreach. Copyright 2008 President and Fellows of Harvard College.
STARTING
MATERIAL
Plasmid with ampicillin resistance gene
and arabinose promoter
Life Sciences-HHMI Outreach. Copyright 2008 President and Fellows of Harvard College.
I
I
STARTING
MATERIAL
GFP
gene
Jellyfish DNA
Life Sciences-HHMI Outreach. Copyright 2008 President and Fellows of Harvard College.
I
STARTING
MATERIAL
Bacterial
chromosome
E. coli cell: competent and sensitive to antibiotics
Life Sciences-HHMI Outreach. Copyright 2008 President and Fellows of Harvard College.
I
STARTING
MATERIAL
Bacterial
chromosome
E. coli cell: competent and sensitive to antibiotics
Life Sciences-HHMI Outreach. Copyright 2008 President and Fellows of Harvard College.
I
Life Sciences-HHMI Outreach. Copyright 2008 President and Fellows of Harvard College.
I
GFP
gene
Life Sciences-HHMI Outreach. Copyright 2008 President and Fellows of Harvard College.
I
Recombinant pGLO plasmid
Life Sciences-HHMI Outreach. Copyright 2008 President and Fellows of Harvard College.
I
pGLO
pGLO
pGLO
Life Sciences-HHMI Outreach. Copyright 2008 President and Fellows of Harvard College.
I
pGLO
pGLO
pGLO
Life Sciences-HHMI Outreach. Copyright 2008 President and Fellows of Harvard College.
I
pGLO
pGLO
pGLO
Life Sciences-HHMI Outreach. Copyright 2008 President and Fellows of Harvard College.
I
nutrient
broth
pGLO
pGLO
pGLO
Life Sciences-HHMI Outreach. Copyright 2008 President and Fellows of Harvard College.
END RESULT:
Recombinant
Bacteria
Next steps:
(1) Select only the
transformed cells
(2) Make them glow!
Life Sciences-HHMI Outreach. Copyright 2008 President and Fellows of Harvard College.
I
I
Life Sciences-HHMI Outreach. Copyright 2008 President and Fellows of Harvard College.
I
Life Sciences-HHMI Outreach. Copyright 2008 President and Fellows of Harvard College.
I
Life Sciences-HHMI Outreach. Copyright 2008 President and Fellows of Harvard College.
I
nutrient
broth
Life Sciences-HHMI Outreach. Copyright 2008 President and Fellows of Harvard College.
END RESULT:
NonRecombinant
Bacteria
Life Sciences-HHMI Outreach. Copyright 2008 President and Fellows of Harvard College.
I
II
Non-Recombinant Bacteria
Life Sciences-HHMI Outreach. Copyright 2008 President and Fellows of Harvard College.
II
Non-Recombinant Bacteria
Life Sciences-HHMI Outreach. Copyright 2008 President and Fellows of Harvard College.
II
Non-Recombinant Bacteria
Life Sciences-HHMI Outreach. Copyright 2008 President and Fellows of Harvard College.
II
Recombinant Bacteria
Life Sciences-HHMI Outreach. Copyright 2008 President and Fellows of Harvard College.
II
Recombinant Bacteria
Life Sciences-HHMI Outreach. Copyright 2008 President and Fellows of Harvard College.
II
Recombinant Bacteria
Life Sciences-HHMI Outreach. Copyright 2008 President and Fellows of Harvard College.
II
LB
LB (nutrients only)
agar plate
Life Sciences-HHMI Outreach. Copyright 2008 President and Fellows of Harvard College.
II
LB
LB (nutrients only)
agar plate
Life Sciences-HHMI Outreach. Copyright 2008 President and Fellows of Harvard College.
II
LB/amp
LB + ampicillin
agar plate
Life Sciences-HHMI Outreach. Copyright 2008 President and Fellows of Harvard College.
II
LB/amp
LB + ampicillin
agar plate
Life Sciences-HHMI Outreach. Copyright 2008 President and Fellows of Harvard College.
II
LB/amp/ara
LB + ampicillin + arabinose
agar plate
Life Sciences-HHMI Outreach. Copyright 2008 President and Fellows of Harvard College.
II
LB/amp/ara
LB + ampicillin + arabinose
agar plate
Life Sciences-HHMI Outreach. Copyright 2008 President and Fellows of Harvard College.
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