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MODULE 9: GENE THERAPY AND TRANSGENIC TECHNOLOGY
Distinguish between retrovirus and retroviral vector.
What are retroviral packaging cell lines?
How is a replication-defective recombinant retrovirus generated?
How can you treat hemophilia by gene therapy?
What is severe combined immunodeficiency syndrome? How can it be treated by gene
therapy?
Discuss the different nonviral methods of delivering genes into mammalian tissues and
mention their advantages and disadvantages.
What is a DNA vaccine? Describe its merits and demerits.
What are the limitations of gene therapy that hinder its clinical success?
Describe the various steps involved in the generation of a transgenic mouse
How are transgenic plants generated?
Name the transgenic crop that is approved for cultivation in India.
Discuss the merits and demerits of transgenic crops.
How are knockout mice generated?
What are conditional knockouts?
Described the technology that was used for the generation of a transgenic rat.
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