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Name____________________________
Punnett Squares:
A Dihybrid Cross
A Dihybrid Cross traces two traits from parents to offspring.
Example 1:
In pea plants, tall stems are dominant over short stems; and Axial flowers are dominant over
Terminal flowers. Two plants are crossbred. The first parent is heterozygous for stem
height and heterozygous for flower type. The second parent is also hybrid for both of these
traits. What genotypes and phenotypes can be expected in the offspring?
a. Step One: Write down gene symbols and what they stand for.
b. Step Two: Write down the parent genotypes.
c. Step Three: Complete the Punnett Square (use the one provided below).
d. Step Four: Summarize the genotype and phenotype results.
Genotype and Phenotype Results of Cross:
Example 2: (On your own)
In cats, black fur is dominant over white fur and short tails are dominant over long tails. A
heterozygous black fur cat with a long tail is mated with a homozygous black fur cat, which
has a heterozygous short tail. What genotypes and phenotypes can be expected in the
offspring?
Name_____ANSWER KEY________
Punnett Squares:
A Dihybrid Cross
A Dihybrid Cross traces two traits from parents to offspring.
Example 1:
In pea plants, tall stems are dominant over short stems; and Axial flowers are dominant over
Terminal flowers. Two plants are crossbred. The first parent is heterozygous for stem
height and heterozygous for flower type. The second parent is also hybrid for both of these
traits. What genotypes and phenotypes can be expected in the offspring?
a. Step One: Write down gene symbols and what they stand for.
A = Axial
a = terminal
T = Tall
t = short
b. Step Two: Write down the parent genotypes.
Parent 1 = AaTt
Parent 2 = AaTt
c. Step Three: Complete the Punnett Square (use the one provided below).
d. Step Four: Summarize the genotype and phenotype results.
AT
At
aT
at
AT
At
aT
at
AATT
AATt
AaTT
AaTt
AATt
AAtt
AaTt
AAtt
AaTT
AaTt
aaTT
aaTt
AaTt
Aatt
aaTt
aatt
Genotype and Phenotype Results of Cross:
Genotype
AATT 1/16
Phenotype
Axial, tall 9/16
AATt
AAtt
AaTT
AaTt
Aatt
aaTT
aaTt
aatt
2/16
1/16
2/16
4/16
2/16
1/16
2/16
1/16
Axial, short 3/16
Terminal, tall
3/16
Terminal, short 1/16
Phenotypic ratio 9:3:3:1
Example 2: (On your own)
In cats, black fur is dominant over white fur and short tails are dominant over long tails. A
heterozygous black fur cat with a long tail is mated with a homozygous black fur cat, which
has a heterozygous short tail. What genotypes and phenotypes can be expected in the
offspring?
Parent 1 = Bbss
Parent 2 = BBSs
BS
Bs
BS
Bs
Genotype
BBSs 4/16
BBss 4/16
BbSs 4/16
Bbss 4/16
Bs
BBSs
BBss
BBSs
BBss
Bs
BBSs
BBss
BBSs
BBss
bs
BbSs
Bbss
BbSs
Bbss
bs
BbSs
Bbss
BbSs
Bbss
Phenotype
Black, short ½
Black, long ½
Black, short
Black, long
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