Download 7, 2012

Changhong Li M.D.,Ph.D. CV
The Children's Hospital of Philadelphia
University of Pennsylvania
CURRICULUM VITAE
Changhong Li, Ph.D., M.D. Assistant Professor
7, 2012
Office Address:
802B, ARC
The Children's Hospital of Philadelphia
3615 Civic Center Boulevard
Philadelphia, PA 19104
Phone 215-590-5380, FAX 215-590-1605, [email protected]
Education:
1984-1989
1991-1996
Medical B.A. ChengDe Medical Collage, CHINA
M.D. and Ph.D., Beijing Medical University, CHINA
Postgraduate Training and Fellowship Appointments:
1989-1991
1998-1999
2000-2003
Resident in Internal Medicine, the Fifth Teaching Hospital of Hebei Medical
University, CHINA
Post-doc Fellowship, Lipids and Growth, Faculty of Medicine, INSERM (U921),
Tours University, FRANCE
Post-doc Fellowship, Endocrinology, Children's Hospital of Philadelphia and
Diabetes center, University of Pennsylvania
Working Experience:
1997-2000
2003-2009
2010-now
Attending Doctor, Department of internal medicine, The Fifth Teaching Hospital
of Hebei Medical University, CHINA
Research Scientist, Division of Endocrinology, Children’s Hospital of Philadelphia
Research Assistant Professor, Department of Pediatrics, Children’s Hospital of
Philadelphia and University of Pennsylvania
Memberships in Professional:
American Diabetes Association (ADA)
American Society for Biochemistry and Molecular Biology (ASBMB)
Federation of American Societies for Experimental Biology (FASEB)
Metabolomics Society
Awards:
1. Outstanding Poster Award, University of Pennsylvania, Diabetes Center Spring
Symposium (03/19/2002).
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Changhong Li M.D.,Ph.D. CV
2. Travel Award, Research Symposium on Islet Biology, October 2002, Falmouth,
Massachusetts
3. Travel Grand Award, The 63rd Scientific Sessions of American Diabetes Association,
June 13-17, 2003, New Orleans
4. Outstanding general poster award, University of Pennsylvania, Institute for Diabetes,
Obesity and Metabolism, Spring Symposium, 3/11/2009
5. Young Investigator Travel Grant Award, the 69th Scientific Sessions of American
Diabetes Association, June 5-9, 2009, New Orleans
6. Winner of Poster day at Children’s Hospital of Philadelphia, Feb 24, 2010
Lectures by Invitation:
1. July 19, 2007. “Congenital Hyperinsulinism”. The first Hospital of Hebei Medical
University, Shijiazhuang, Hebei, China
2. July 20, 2007: “Regulation of Insulin Secretion”. The First Hospital of Peking
University. Beijing, China
3. April 6, 2009: “Amino acid stimulated insulin secretion and beyond”. The
Children’s Hospital of Philadelphia, Mitochondria Affinity Group Seminar
4. October 9, 2009, “Insulin and glucagon secretion in type 2 diabetic human islets”.
Seminar in the First Hospital of Peking University. Beijing, China
5. October 11, 2009, “Studies of hyperinsulinism mouse model”, 2009 Beijing Children’s
Hospital Endocrinology Forum and class, Beijing, China
6. April 26, 2010, “Amino acid and glucose homeostasis---the HI story”, Experimental
Biology 2010 Symposium, “Resent and Emerging Clinical Application of Amino
Acids”, Anaheim, California
7. May 8, 2010, “Regulation of glucagon secretion”, 2010 Beijing Children’s Hospital
Endocrinology Forum and class, Beijing, China
8. March 15, 2012, “Amino acid stimulated insulin secretion in hyperinsulinism mouse
models”, 2012 March 15 to 16th, Symposium: Monogenic Disorders of Insulin Secretion:
Congenital Hyperinsulinism and Neonatal Diabetes.
9. June, 8th, 2012. “Islet paracrine/autocrine small molecular signals”. Symposium of intraislet communication. 72nd Scientific Section, American Diabetes Association.
Bibliography:
Publications:
1. Changhong Li, Chengyang Liu, Itzhak Nissim, Jie Chen, Pan Chen, Nicolai Doliba, Ilina
Nissim, Yevgeny Daikhin, David Stokes, Marc Yudkoff, Michael J. Bennett, Charles A.
Stanley, Franz M. Matschinsky, Ali Naji. Regulation of Glucagon Secretion in Normal
and Diabetic Human Islets by Gamma-Hydroxybutyrate and Glycine. Under
Review at JBC. 2012.
2. Yan Gao, Daniel J. Sartori, Changhong Li, Qian-Chun Yu, Jake A. Kushner, J. Alan
Diehl. PERK is required in the adult pancreas and is essential for maintenance of
glucose homeostasis. Under review in PNAS, 2012
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Changhong Li M.D.,Ph.D. CV
3. Andrew C. Calabria, Changhong Li, Paul R. Gallagher, Charles A. Stanley, and Diva D.
De León. GLP-1 Receptor Antagonist Exendin-(9-39) Elevates Fasting Blood Glucose
Levels in Congenital Hyperinsulinism Owing to Inactivating Mutations in the ATPSensitive K+ Channel. Diabetes. August 1 2012 (published online)
4. Srinivas B Narayan, Stephen R Master, Anthony N Sireci, Charlene Bierl, Paige E
Stanley, Changhong Li, Charles A Stanley, Michael J Bennett. Short-Chain 3Hydroxyacyl-Coenzyme A Dehydrogenase Associates with a Protein Super-Complex
Integrating Multiple Metabolic Pathways. PLoS ONE. Vol: 7 (4); Pages: e35048. 2012.
5. Ming Li, Changhong Li, Aron Allen, Charles A. Stanley, Thomas J. Smith. The structure
and allosteric regulation of mammalian glutamate dehydrogenase. Archives of
Biochemistry and Biophysics, 519(2):69–80, 2012.
6. Doliba NM, Qin W, Najafi H, Liu C, Buettger CW, Sotiris J, Weik-Collins H, Li C, Stanley
CA, Wilson DF, Grimsby J, Sarabu R, Naji A, Matschinsky FM.Glucokinase activation
repairs defective bioenergetics of islets of langerhans isolated from type 2 diabetics. Am
J Physiol Endocrinol Metab, 302(1):E87-E102. (Epub 2011 Sep 27). 2012.
7. Changhong Li, Ming Li, Pan Chen, Srinivas Narayan, Franz M. Matschinsky, Michael J.
Bennett, Charles Stanley, and Thomas J. Smith. Green tea polyphenols control
dysregulated glutamate dehydrogenase in transgenic mice by hijacking the ADP
activation site. J. Biol. Chem. 2011 Sep 30;286(39):34164-74. Epub 2011 Aug 3.
8. Matschinsky FM, Zelent B, Doliba N, Li C, Vanderkooi JM, Naji A, Sarabu R, Grimsby J.
Glucokinase Activators for Diabetes Therapy: May 2010 status report. Diabetes Care.
2011 May; 34 Suppl 2:S236-43.
9. Ming Li, Changhong Li, Aron Allen, Charles A. Stanley and Thomas J. Smith. The
structure and allosteric regulation of glutamate dehydrogenase. Neurochem Int. 2011,
59: 445-455 (2010 Nov 9. Epub ahead of print)
10. Changhong Li, Pan Chen, Andrew Palladino, Srinivas Narayan, Laurie K. Russell,
Samir Sayed, Guoxiang Xiong, Jie Chen, David Stokes, Yasmeen M. Butt, Patricia M.
Jones, Heather W. Collins, Noam A. Cohen, Akiva S. Cohen, Itzhak Nissim, Thomas J.
Smith, Arnold W. Strauss, Franz M. Matschinsky, Michael J. Bennett, Charles A. Stanley.
Mechanism of hyperinsulinism in short-chain 3-hydroxyacyl-CoA dehydrogenase
deficiency involves activation of glutamate dehydrogenase. J. Biol. Chem. 285: 3180631818. 2010.
11. Diva D. Deleon, Changhong Li, Madeleine Delson, Charles A. Stanley, Franz M.
Matschinsky, Doris A. Stoffers. Exendin (9-39) corrects fasting hypoglycemia in sur-1-/mice by lowering cAMP in pancreatic beta-cells and inhibiting insulin secretion. J. Biol.
Chem. 283:25786-25793, 2008
12. Changhong Li, Itzhak Nissim, Pan Chen, Carol Buettger, Habiba Najafi, Yevgeny
Daikhin, Ilana Nissim, Heather W. Collins, Marc Yudkoff, Charles A. Stanley and Franz
M. Matschinsky. Elimination of KATP channels in mouse islets results in elevated [U13C]glucose metabolism, glutaminolysis and pyruvate cycling but a decreased GABA
shunt. J. Biol. Chem. 283: 17238-17249, 2008
13. Maria F. Pino, Kyoung-Ah Kim, Kathy D. Shelton, Jill Lindner, Stella Odili, Changhong
Li, Heather W. Collins, Masakazu Shiota, Franz M. Matschinsky, and Mark A.
Magnuson. Glucokinase thermolability and hepatic regulatory protein binding are
essential factors for predicting the blood glucose phenotype of missense mutations. J.
Biol. Chem. 282: 13906-13916, 2007
14. Changhong Li, Andrea Matter, Andrea Kelly, Thomas J. Petty, Habiba Najafi, Courtney
MacMullen, Yevgeny Daikhin, Ilana Nissim, Adam Lazarow, Jae Kwagh, Heather W.
Collins, Betty Y.L. Hsu, Itzhak Nissim, Marc Yudkoff, Franz M. Matschinsky and Charles
A. Stanley. Effects of a GTP-insensitive mutation of glutamate dehydrogenase on insulin
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Changhong Li M.D.,Ph.D. CV
secretion in transgenic mice. J. Biol. Chem. Vol. 281: 15064-15072, 2006. (Paper of the
week, also illustrated on the cover of June 2nd issue.)
15. Changhong Li, Aron Allen, Jae Kwagh, Nicolai Doliba, Wei Qin, Habiba Najafi, Heather
W. Collins, Franz M. Matschinsky, Charles A. Stanley, and Thomas J. Smith. Green tea
polyphenols modulate insulin secretion by inhibiting glutamate dehydrogenase. J. Biol.
Chem. Vol. 281: 10214-10221.2006.
16. Jacques Delarue, Chang-Hong Li, Richard Cohen, Charlotte Corporeau, Brigitte Simon.
Interaction of fish oil and a glucocorticoid on metabolic responses to an oral glucose load
in healthy human subjects. Br J Nutr. 2006. 95(2): 267-272
17. D. Zelent, H. Najafi, S. Odili, C. Buettger, H. W. Collins, C, Li, N. Doliba, J. Grimsby and
F.M. Matschinsky. Glucokinase and Glucose Homeostasis: Proven Concepts and New
Ideas. Biochem Soc Trans. 2005 Feb;33(Pt 1):306-10.
18. Nicolai M. Doliba, Wei Qin, Marko Z. Vatamaniuk, Changhong Li, Dorothy Zelent,
Habiba Najafi, Carol W. Buettger, Heather W. Collins, Richard D. Carr, Mark A.
Magnuson, and Franz M. Matschinsky. Restitution of Defective Glucose-Stimulated
insulin Release of Sulfonylurea Type 1 Receptor Knockout Mice by Acetylcholine. Am J
Physiol Endocrinol Metab Vol. 286(5):E834-43. Epub 2004 Jan 21, 2004
19. Changhong Li, Carol Buettger, Jae Kwagh, Andrea Matter, Yevgeny Daikhin, Ilana B.
Nissim, Heather W. Collins, Marc Yudkoff, Charles A. Stanley, and Franz M.
Matschinsky. A signaling role of glutamine in insulin secretion. J. Biol. Chem. Vol. 279:
13393-13401,2004
20. Changhong Li, Habiba Najafi, Yevgeny Daikhin, Ilana Nissim, Heather Collins, Marc
Yudkoff, Franz M. Matschinsky, Charles A. Stanley: Regulation of Leucine-stimulated
Insulin Secretion and Glutamine Metabolism in Isolated Rat Islets. J. Biol. Chem. Vol.
278: 2853-2858, 2003
21. Andrea Kelly, Changhong Li, Zhiyong Gao, Charles A. Stanley, Franz M. Matschinsky.
Glutaminolysis and Insulin Secretion: From Bedside to Bench and Back. Diabetes,
Vol.51, (Supp 3): S421-S426, 2002
Chapter of Books:
1.
2.
3.
Changhong Li, Franz M. Matschinsky, Charles Stanley. Chapter: Amino acid stimulated
insulin secretion: the role of glutamine-glutamate-a-ketoglutarate axis. Frontiers in
Diabetes. Monogenic Hyperinsulinemic Hypoglycemia Disorders. Vol 21. (112-124).
Karger. 2012
Andrea Kelly, Changhong Li. Chapter: Leucine sensitivity. Encyclopedia of Molecular
Mechanisms of Disease. Springer (Editor in chief: Florian Lang). 2009
Andrea Kelly, Changhong Li, Charles A. Stanley: Chapter: Glucokinase/ glutamate
dehydrogenase interactions in the GDH form of Congenital Hyperinsulinism. Glucokinase
and Glycemic Disease: from the Bedside to Novel Therapeutics. Frontiers in Diabetes,
2004. Vol 16, P110-124 Basel, Karger. (Matschinsky FM, Magnuson MA, eds)
Abstract Publications:
1. PUJA PATEL, CHANGHONG LI, STEPHANIE GIVLER, FRANZ M. MATSCHINSKY,
CHARLES STANLEY, DIVA D. DE LEON. Functional Evaluation of Islets from Infants with
KATP Hyperinsulinism. Diabetes. Vol 61 (Suppl 1), 2012. A11.
2. MATTHEW M. RANKIN, KOURTNEY B. KING, JENNY J. KIM, JUSTIN X. TU,
CHANGHONG LI, JAKE A. KUSHNER. New ß-cells are Not Generated by Prolonged
Administration of Incretin-Based Therapies (Exendin-4 or Sitagliptin) in Adult Mouse
Pancreas. Diabetes. Vol 61 (Suppl 1), 2012. Lb34.
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Changhong Li M.D.,Ph.D. CV
3. CHANGHONG LI, PUJA PATEL, TINGTING ZHANG, PAN CHEN, STEPHANIE GIVLER,
CHENGYANG LIU, ITZHAK NISSIM, ALI NAJI, FRANZ M. MATSCHINSKY, DIVA D. DE
LEÓN, CHARLES STANLEY. [U-13C]-Glucose Metabolism in Islets From Infants With
KATP Hyperinsulinism. Diabetes. Vol 61 (Suppl 1), 2012. A536.
4. NICOLAI M. DOLIBA, QING LIU, WEI QIN, INDERROOP SINGH, CHENGYANG LIU,
CHANGHONG LI, ALI NAJI, FRANZ M. MATSCHINSKY. Acute and Chronic Effects of Fatty
Acids on Acetylcholine- and Exendin-4-Stimulated Insulin Secretion in Mouse and Human
Pancreatic Islets. Diabetes. Vol 61 (Suppl 1), 2012. A537.
5. DANIEL J. SARTORI, CHRISTOPHER J. WILBUR, SIMON LONG, CHANGHONG LI,
WILLIAM T. PU, JAKE A. KUSHNER. Gata Transcription Factors Promote Endoplasmic
Reticulum Integrity and Pancreatic ß-cell Survival. Diabetes. Vol 61 (Suppl 1), 2012. A522.
6. DANIEL R. JACOBSON, MATTHEW M. RANKIN, ALISA B. SCHIFFMAN, CHANGHONG
LI, JAKE A. KUSHNER. ß-Cells Persist in Some T1DM Pancreata Without Evidence of ßCell Turnover Nor Insulin-Glucagon Co-Expression. Diabetes. Vol 61 (Suppl 1), 2012. A531.
7. Changhong Li, Chengyang Liu, Itzhak Nissim, Jie Chen, Pan Chen, Nicolai Doliba, Ilina
Nissim, Yevgeny Daikhin, David Stokes, Marc Yudkoff, Michael J. Bennett, Charles A.
Stanley, Franz M. Matschinsky, Ali Naji. The role of GABA shunt and r-hydroxybutyrate
(GHB) in regulation of glucagon secretion in human islets. Diabetes. Vol 60 (Suppl 1), 2011.
A97.
8. Changhong Li, Pan Chen, Andrew Palladino, Srinivas Narayan, David Stokes, Arnold W.
Strauss, Franz M. Matschinsky, Michael J. Bennett, Charles A. Stanley. Dysregulation of
insulin secretion in short-chain 3-hydroxyacyl-CoA dehydrogenase (SCHAD) knockout mice.
Diabetes. Vol 58 (Suppl 1), 2009. A1.
9. Changhong Li, Andrew Palladino, Srinivas Narayan, Pan Chen, Arnold W. Strauss, Michael
J. Bennett, Charles A. Stanley. Mechanism of hyperinsulinemia in SCHAD deficiency:
studies of insulin dysregulation in SCHAD-/- mice. Hormone Research. Vol 72, S3, 2009,
62.
10. Changhong Li, Chengyang Liu, Itzhak Nissim, Pan Chen, Nicolai Doliba, Ilana Nissim,
Yevgeny Daikhin, David Stokes, Marc Yudkoff, Charles A. Stanley, Franz M. Matschinsky,
Ali Naji. Islets of human type 2 diabetics have decreased GABA shunt and dysregulated
glucose metabolism. Diabetes. Vol 58 (Suppl 1), 2009. A424.
11. Richard D. Kibbey, Cheol Sod Choi, Hui-Young Lee, Over Cabrera, Andreas Birkenfeld,
Changhong Li, Verman Samuel, Charles Stanley, Gernald I. Shulman. -cell glutamate
dehydrogenase regulates glucagon release from -cells in vivo. Diabetes. Vol 58 (Suppl 1),
2009. A59.
12. Changhong Li, Shaoping Deng, Jing Wang, Wei Qin, Ali Naji, Charles A. Stanley, Franz M.
Matschinsky. Regulation of ATP release from human and mouse islets. Diabetes, Vol 57
(Suppl 1): A464-A465, 2008
13. Li C, Chen P, Narayan SB, Qin W, Chen J, Matschinsky FM, Strauss AW, Bennett MJ,
Stanley CA. Evidence of dysregulated insulin secretion in mice with global knockout of shortchain 3-hydroxy acyl-CoA dehydrogenase. Molecular Genetics and Metabolism. Vol. 90,
256, 2007
14. Changhong Li, Itzhak Nissim, Pan Chen, Carol Buettger, Habiba Najafi, Yevgeny Daikhin,
Ilana Nissim, Heather W. Collins, Marc Yudkoff, Charles A. Stanley and Franz M.
Matschinsky. Chronic elevation of cytosolic calcium impairs the GABA shunt in pancreatic
islets of SUR1 knockout mice --- studies with U-13C-glucose. Diabetes, Vol. 56 (Suppl 1),
A439, 2007.
15. Nicolai M. Doliba, Wei Qin, Changhong Li, Heather W. Collins, Franz M. Matschinsky. Acute
inhibition of islet respiration and acetylcholine responsiveness by fatty acids. Diabetes, Vol.
56 (Suppl 1), A440, 2007.
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Changhong Li M.D.,Ph.D. CV
16. Pan Chen, Changhong Li, Yanmei Sang, Carol Buettger, Heather W. Collins, Franz M.
Matschinsky and Charles A. Stanley. The role of glutamine-glutamate--ketoglutarate axis in
amino acid stimulated insulin secretion in the -HC9 cells. Diabetes, Vol. 56 (Suppl 1), A435,
2007.
17. Changhong Li, Andrea Matter, Pan Chen, Nicolai Doliba, Wei Qin, Habiba Najafi, Heather
W. Collins, Thomas J. Smith, Franz M. Matschinsky and Charles A. Stanley. Effects of
epigallocatechin gallate (EGCG) in islets expressing a glutamate dehydrogenase with a gain
of function mutation. Diabetes. Vol. 55 (Suppl 1), A376, 2006
18. Changhong Li, Aron Allen, Jae Kwagh, Wei Qin, Heather W. Collins, Franz M. Matschinsky,
Charles A. Stanley, and Thomas J. Smith. Inhibition of Glutamate Dehydrogenase
Potentiates Glucose Stimulated Insulin Secretion by Rat Islets. Diabetes, Vol. 54 (Suppl 1)
A 417, 2005
19. Changhong Li, Andrea Matter, Habiba Najafi, Yevgeny Daikhin, Ilana B. Nissim, Adam
Lazarow, Heather W. Collins, Itzhak Nissim, Marc Yudkoff, Franz M. Matschinsky and
Charles A. Stanley. Rate and Direction of Glutamate Dehydrogenase Flux in Isolated Mouse
Islets Measured with [C2-15N]-Glutamine and [15N]-Ammonia. Diabetes, Vol. 53 (Suppl 2) A
398, 2004
20. Changhong Li, Nicolai Doliba, Carol Buettger, Jae Kwagh, Andrea Matter, Heather W.
Collins, Charles A. Stanley and Franz M. Matschinsky: A signaling role glutamine in insulin
secretion. Diabetes, Vol. 52 (Suppl 1) A3, 2003
21. Nicolai Doliba, Wei Qin, Marko Vatamaniuk, Changhong Li, Carol Buettger, Heather W.
Collins, Mark Magnuson, Franz M. Matschinsky: Alterations of fuel responsiveness in
pancreatic islets associated with hypersensitivity to acetycholine and IBMX in sulfonylurea
type I receptor knockout mice. Diabetes, Vol. 52 (Suppl 1) A376, 2003
22. Marko Vatamaniuk, Dorothy Zelent, Habiba Hajafi, Nicolai Doliba, Changhong Li, Heather
W. Collins, Carol Buettger, Franz M. Matschinsky. The KATP channel independent pathway
of glucose stimulated insulin release (GSIR) is impaired in sulfonylurea receptor type I
deficient (SUR1-/-) mice. Diabetes, Vol. 52 (Suppl 1) A374, 2003
23. Changhong Li, Habiba Najafi, Yevgeny Daikhin, Ilana Nissim, Heather Collins, Marc
Yudkoff, Franz M. Matschinsky, Charles A. Stanley: Regulation of Glutamine Metabolism
and Insulin Secretion in Isolated Rat Islets. Diabetes vol. 51 (Suppl. 2),A1, 2002
24. Andrea Kelly, Changhong Li, Andrea Matter, Heather W. Collins, Franz M. Matschinsky,
Charles A. Stanley: Amino Acids Stimulated Insulin Secretion in Mice Expressing a
Glutamate Dehydrogenase Transgene with a Gain of Function Mutation. Diabetes vol. 51
(Suppl. 2),A13, 2002
25. Andrea Kelly, Diva NG, Changhong Li, Courtney MacMullen, Franze M Matschinsky,
Charles A Stanely: Molecular Basis of the Hyperinsulinism/Hyperammonemia Syndrome:
Regulation of Glutamate Dehydrogenase by the Energy State of the beta-Cell. Pediatric
Research. Vol 49. (6) (Suppl.): 90A, 2001
26. Changhong Li, Heather W. Collins, Franz M. Matschinsky, Charles A. Stanley: The effects of
beta-cell "fuel depletion" on leucine stimulated insulin secretion (LSIS). Diabetes. Vol. 50
(Suppl. 2): A356, 2001
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