Download Supplementary Table 1. Plant polysaccharide

Supplementary Table 1. Plant polysaccharide-degrading enzymes screened from rumen libraries using a functional
metagenomics approach.
Target activity
Endoglucanase
Library Library size Mb
Hits
type
(# screened clones)
λ phage 1100 (14000;
22
representing ~7%
of the total library)
Activity or substrate degraded
Salient characteristic
Family
Animal
9 endoglucanases (ostazin brilliant
red-hydroxyethyl cellulose)
Novel endoglucanase?
(low homology to known
endoglucanases and esterases)
Novel esterases
High amylase and pullulanase
activities
Highly active laccase with low
homology to know laccases.
Novel family proposed
Acidic cellulose
GH5 & GH26
Cattle
(cow)
GH5
(cellulase)
buffalo
(Duan et al., 2009)
Double catalytic domain
hydrolase:a) β-1,4-mannanase
and b) β-1,4-xylanase and β1,4-glucanase
GH5 & GH26
+ CBM
Cattle
(cow)
(Palackal et al.,
2007)
GH5
Cattle
(cow)
(Shedova et al.,
2009)
Esterase
Cyclodextrinase
12 esterases (α-naphthyl acetate)
1 dextrinase (α-cyclodextrin)
Polyphenol
oxidase
Syringaldazine, 2,6-dimetoxyphenol,
veratryl alcohol, guaiacol, and 4metoxybenzyl alcohol among others
14 endoglucanase
47 -glucosidases
Endoglucanase
-glucosidase,
Cellodextrinase
Endoglucanase
Cosmid 525 (15000)
61
λ phage NA1 (50000 plates)
phage
1
Mannanase, glucanase and
xylanase
Endoglucanase
Plasmid 90 (6000)
35
Lipases
BAC
837 (15360)
NA
Endoglucanase and lichenase
actitivy
-xylosidase (ORF)
Several clones with amylase,
endoglucanase, xylanase,
cellobiosidase, and lipase activity (2
lipases characterised).
Endoglucanase
Cosmid 650 (15600)
46
Xylanaseendocellulase
Cosmid NA (4000)
3
Lipase
Xylanase
Amylase
Plasmid NA
BAC
NA (15360)
24
1
NA: information not available
41 β-glucosidase
5 glucanase (1 endoglucanase
characterised)
1 characterised
2
18
10
High activity for long-chain fatty
acid substrates for the lipases
bifunctional glycosyl hydrolase
domain with β-1, 4-xylosidase
and β-1,4-glucosidase activity
Proposed new lipase family
Double endo-β-1,4-xylanase
domains
Low similarity to other amylases
Reference
(Ferrer et al.,
2005)
(Ferrer et al.,
2007)
(Beloqui et al.,
2006)
GH13
GH3
One enzyme
Cattle (Zhu et al., 2007,
Liu et al., 2009a)
might be
(cow)
classified as a
new subfamily
of true lipases
(EC 3.1.1.3)
GH5
Buffalo (Liu et al., 2009b)
GH5
Yak
(Chang et al.,
2011)
GH43, CBM4
GH57
Sheep
Cattle
(cow)
(Bayer et al., 2010)
(Zhao et al., 2010)