Download Assiut university researches Blepharisides A and B, new flavonol

Assiut university researches
Blepharisides A and B, new flavonol glycosides
from Blepharis ciliaris growing in Saudi Arabia
Gamal A. Mohamed, Sabrin R.M. Ibrahim, Ehab S. Elkhayat, Samir A.
Ross, Hanaa M. Sayed, Safaa A.M. El-Moghazy, Mohamed A. ElShanawany
Abstract:
Bio-guided fractionation of the total MeOH extract (TME) of the
aerial parts of Blepharis ciliaris (L.) B.L. Burtt. (Acanthaceae)
growing in Saudi Arabia was carried out to evaluate its
hepatoprotective activity against CCl4-induced hepatotoxicity in
rats. Successive chromatographic separations of the potent
hepatoprotective n-BuOH fraction afforded two new flavonol
glycosides, blepharisides A (1) and B (2), along with quercetin
3-O-rutinoside (3). Their structures were established by UV, IR,
1D, 2D NMR, and HRESIMS spectral data, in addition to
comparison with literature data. Co-treatment of CCl4 hepatic
injured rats with the total MeOH extract (TME) and its fractions
significantly restored the hepatic marker enzymes and total
bilirubin to near-normal values compared to silymarin (reference
drug). The isolated compounds were evaluated for their
antioxidant and anti-inflammatory activities. They displayed
significant antioxidant activity (DPPH assay) in relation to propyl
gallate (positive control) (% inhibition of 88.2, 87.9, and 74.2,
respectively). Compounds 1 and 2 demonstrated antiinflammatory effects in the carrageenin induced paw edema
method at a dose of 10 mg/kg.
Key words:
Blepharis ciliaris; Acanthaceae; Blepharisides;
Hepatoprotective; Antioxidant; Anti-inflammatory
Published in:
Phytochemistry Letters,vol. 11,pp. 177–182