Download Volvox carteri ft. nagariensis

Table S1. Annotated cycloartenol synthase orthologs in microalgal genomes.
Organism
Ostreococcus tauri
Green Algae
Ostreococcus lucimarinus
Green Algae
Micromonas pusilla
Green Algae
Micromonas sp. RCC299
Green Algae
Chlamydomonas reinhardtii
Green Algae
Volvox carteri ft. nagariensis
Green algae
Chlorella variabilis
Green algae
Thalassiosira pseudonana
Diatom
Phaeodactylum tricornutum
Diatom
Fragilariopsis cylindrus
Diatom
Aureococcus anophagefferens
Brown tide algae
Nannochloropsis gaditana
Eustigmatophyte
Aphanomyces euteiches
Oomycete
Ectocarpus siliculosus
Cyanidioschyzon merolae
Red Algae
Chondrus crispus
Red Algae
Galdieria sulphuraria
Red Algae
Emiliania huxleyi 1516
Haptophyte
Ortholog
Accession number
XP_003077997.1*
XP_001416570.1
XP_003055963.1
XP_002507650.1
XP_001689874.1*
XP_002955292.1
EFN56189.1
XP_002287432.1*
XP_002185678.1
Scaffold 9
EGB12462.1
GAGR01001964.1
CAQ55984.1
CBN75619.1*
BAM80160.1*
CDF35754.1*
EME26352.1*
Scaffold 360
Taxonomy
Viridiplantae; Chlorophyta;
Mamiellophyceae
Viridiplantae; Chlorophyta;
Mamiellophyceae
Viridiplantae; Chlorophyta;
Mamiellophyceae
Viridiplantae; Chlorophyta;
Mamiellophyceae
Viridiplantae; Chlorophyta;
Chlorophyceae;
Viridiplantae; Chlorophyta;
Chlorophyceae;
Viridiplantae; Chlorophyta;
Trebouxiophyceae
Stramenopiles; Bacillariophyta;
Coscinodiscophyceae;
Stramenopiles; Bacillariophyta;
Bacillariophyceae;
Stramenopiles; Bacillariophyta;
Bacillariophyceae;
Stramenopiles; Pelagophyceae;
Aureococcus
Stramenopiles;
Eustigmatophyceae;
Eustigmatales
Stramenopiles; Oomycetes;
Saprolegniales
Stramenopiles; PX clade;
Phaeophyceae; Ectocarpales
Rhodophyta; Bangiophyceae;
Cyanidiales
Rhodophyta; Florideophyceae;
Gigartinales
Rhodophyta; Bangiophyceae;
Cyanidiales
Haptophyceae; Isochrysidales;
Noelaerhabdaceae
*annotated as cycloartenol synthase coding gene. The rest of the proteins were annotated as putative CS in
this study based on percentage of identity with CS orthologs in other taxa. Peptide sequences matching
query sequences with E-value <1e-150 and aligning over 55% of the query protein length were considered as
significant.
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Table S2. List of primers used for the detection of CS gene coding sequences.
Name
Protein sequence
Primer (5’-3’)
Name
Cycloart_F
WLLPNWF
(146–152 aa)*
TGGCTKCTMCCMAACTGGTTT
Cycloart_R
CycloF_TPF
PNW(F/I)PFHP
(149–156 aa)
CCMAACTGGWTTCCTTTYCATC
CycloR_TPF
Protein
sequence
GYNGSQC
(310–316 aa)
GYNGSQC
(310–316 aa)
Primer
G[CATTGGCTKCCGTTRTAGCC]
[CAYTGGCTKCCGTTRTAKCC]†
*amino acid (aa) positions of the cycloartenol synthase of T. pseudonana (Accession number XP_002287432.1). K = G/T; M = A/C; W = A/T; Y = C/T.
†Primer CycloR_TPF has one nucleotide less than Cycloart_R primer but we indicate the same amino acid motif for reference.
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Table S3. Diatom cultures investigated in this study.
Species
Thalassiosira pseudonana CCMP 1335
Thalassiosira gravida CCMP 986
Thalassiosira aff antarctica CCAP 1085/9
Fragilaropsis cylindrus CCMP 1102
Pseudo-nitzschia seriata CCMP 1309
Extubocellulus spinifer CCMP 393
Extubocellulus cribiger CCAP 1026/1
Phaeodactylum tricornutum
Attheya septentrionalis CS 425/03
Skeletonema costatum CCMP 1281
Skeletonema subsalsum CCAP 1077/8
Proboscia indica CCMP 1896
Proboscia inermis CCAP 1064/1
Proboscia alata
Sterol composition*
C28 11(85), 15(6); C29 25(5), 26(4)
C28 11(95); C29 25(5)
C27 2(4); C28 11(83); C29 25(3)
C27 2(68), 5(32)
C27 2(82), 5(18)
C27 2(79), 5(2); C28 10(13), 11(1), 13(1), 14(1); C29
29(3)
C27 2(74), 5(4); C28 10(12), 11(4), 13(1), 15(2); C29
29(3)
C28 10(99), 15(1)
C27 5(7), 5(2); C28 11(37), 15(19); C29 26(tr), 35(38)
C27 3(5), 5(8), 8(1); C28 11(73), 18(11); C29 25(1),
26(1)
C27 3(10), 5(44); C28 11(24), 15(5); C29 25(tr),
26(11), 35(6)
C28 11(90), 18(10)
C27 5(44); C28 11(56)
C27 3(34), 5(29); C28 11(35); C29 24(2)
PCR
result
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*Data from Rampen et al., 2010.: Numbers in front of parentheses correspond to the sterol numbers as it follows: [2 (cholesta-5,22E-dien-3β-ol); 3 (cholesta5,24-dien-3β-ol); 5 (cholest-5-en-3β-ol); 8 (5α-cholestan-3β-ol); 10 (24-methylcholesta-5,22E-dien-3β-ol); 11 (24-methylcholesta-5,24(28)-dien-3β-ol); 13 (23methylcholesta-5,22E-dien-3β-ol); 14 (23-methylcholesta-5,23(28)-dien-3β-ol); 15 (24-methylcholesta-5-en-3β-ol); 18 (24-methylcholesta-24(28)-en-3β-ol); 24
(24-ethylcholesta-5,22E-dien-3β-ol); 25 (24-ethylcholesta-5,24(28E)-dien-3β-ol); 26 (24-ethylcholesta-5,24(28Z)-dien-3β-ol); 29 (23,24-dimethylcholesta5,22E-dien-3β-ol); 35 (24-ethylcholest-5-en-3β-ol)]. Values in parentheses represent the concentration of the individual sterol, as a percentage of the total sterols.
tr indicates relative abundances of < 0.5%.