Download 葉部形態的研究一直是植物學家努力的目標

Title：Functional study of a T-DNA insertional mutant with serrated leaves in
Arabidopsis
M.S. Student： Yin-JU Ho (R91226008)
Advisor：Dr. Tsung-Luo Jinn
Abstract：
The research of leaf morphology in plant science is still in great demand so far. The
development of leaf in plant is influenced by a lot of factors, for example: the polar growth,
distribution of phytohormone, cell differentiation, etc. Besides, leaf is the specific organ in
plant but not in animal. Therefore, we summarize three major points resulting in research
difficulty, first, the functions and mechanisms of leaf development are too complicated for
studying; second, there are no analogous genes in animal system to be compared with ; third,
most of the gene functions in leaf are related to photosynthesis and respiration. For these
reasons, it’s difficult to isolate specific genes which are functional in leaf growth and
development.
In our laboratory, we used T-DNA containing 35S promoter:Bar:SK for transforming
Arabidopsis to generate and select mutants with abnormal leaf phenotype. From these mutants,
we try to find out (1) the numbers and sites of T-DNA insertion, (2) the influenced genes near
the insertion site, (3) the genes that is really responsible for abnormal leaf phenotype, and (4)
the functions of these genes interested in point (3).
Here, we showed a gain-of-function mutant, TPR8, with more serrated leaves than wild
type started from the fourth true leaves. Southern blot and inversed PCR identified one
T-DNA inserted in about 3.35 Kb upstream of At4g01240. Using RT-PCR, we showed that
At4g01240, At4g01245, and At4g01260 were all upragulated in TPR8 mutant. However,
At4g01240 was most upragulated. By using CaMV 35s promoter to overdrive At4g01240 in
wild type, we could recapitulate the serrated phenotype implicating that the phenotype of
TPR8 may result from At4g01240 upragulation, meanwhile, we also found that the gene
product of At4g01240 is a hypothetical protein with unknown function. We will be proceeding
the promoter:GUS transformation and RT-PCR assay to detect where At4g01240 expressed
and study its functions.