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A genome-wide analysis of DNA methylation in buccal - VU-DARE
A genome-wide analysis of DNA methylation in buccal - VU-DARE

... experimental design to examine the extent to which such variation is related to individual-specific environmental influences and stochastic events or to familial factors (DNA sequence and shared environment). We measured genome-wide DNA methylation in buccal samples from ten MZ pairs (age 8-19) usin ...
microbial genetics
microbial genetics

... copy number plasmids, there are enough plasmid molecules for each daughter cell to receive at least some (Fig.4a). The daughter cells do not have to inherit exactly half of the plasmid molecules. Thus, even if a daughter cell only receives a fewer copies of the plasmid than the copy number, this can ...
Multifractal characterisation of length sequences of coding and
Multifractal characterisation of length sequences of coding and

... Claverie [6] also substantially corroborated these results. If one considers more details by distinguishing c from t in pyrimidine, and a from g in purine (such as two- or three-dimensional DNA walk model [7] and maps given in Ref. [8]), then the presence of base correlation can be found even in cod ...
Fulltext PDF - Indian Academy of Sciences
Fulltext PDF - Indian Academy of Sciences

... methylation pattern acquired in response to abiotic or biotic stress is often inherited over one to several subsequent generations. Cytosine methylation marks affect physiological functions of plants via their effect(s) on gene expression levels. They also repress transposable elements that are abun ...
Handout - 7 - Misc - Topics for discussion at June 2016
Handout - 7 - Misc - Topics for discussion at June 2016

... Confusion about the Method “EIA” (aka “a case of unfortunate naming”). The “EIA” method was always intended to cover more than just enzyme-linked immunoassay and was just a convenient shorthand. The display name for the long common name has always been “Immunoassay.” Explanation regarding EIA in the ...
Evaluation of Excessive Pregnancy Weight Gain Effect in Non
Evaluation of Excessive Pregnancy Weight Gain Effect in Non

... Clausen T, Burski TK, Øyen N, Godang K, Bollerslev J, Henriksen T. Maternal anthropometric and metabolic factors in the first half of pregnancy and risk of neonatal macrosomia in term pregnancies. A prospective study. Eur ...
Functions of DNA methylation: islands, start sites, gene bodies and
Functions of DNA methylation: islands, start sites, gene bodies and

... methylated DNA-binding proteins followed by next-generation sequencing. Methyl-DNA immunoprecipitation (MeDIP) is another approach in which extracted DNA is cleaved, denatured and precipitated using an antibody to 5mC, and then the precipitated fragments are sequenced35. Methods based on the treatme ...
GENETIC DISORDERS AND PEDIGREES
GENETIC DISORDERS AND PEDIGREES

... Asians. It affects about 30,000 children and adults in the U.S. b. Deficiency of the protein cystic fibrosis transmembrane conductance regulator (CFTR). This protein is found in the membrane of cells. The normal function of this protein is to transport chlorine across the cell membrane. The dysfunct ...
Coordination of replication and transcription along a Drosophila
Coordination of replication and transcription along a Drosophila

... arrays of cDNAs have demonstrated a correlation between time of replication and the probability that a specific gene is expressed, it remained to be determined what step(s) in the replication initiation process are influenced by transcription. Similarly, because the prior studies lacked contiguous i ...
Understanding Ethnic Disparities of Fetal and Infant Death in Multiple-Gestation Pregnancies
Understanding Ethnic Disparities of Fetal and Infant Death in Multiple-Gestation Pregnancies

... groups of 20-29 years (young), 30-39 years (mature), 40-49 years (very mature), and 50 or more years (older) throughout the United States from 1997-1999. The risks for low birthweight, preterm delivery, and infant mortality was tripled among older mothers, and doubled for very mature mothers. Pregna ...
Eds., N. Hamamura, S. Suzuki, S. Mendo, C. M. Barroso,... © by TERRAPUB, 2010.
Eds., N. Hamamura, S. Suzuki, S. Mendo, C. M. Barroso,... © by TERRAPUB, 2010.

... An increased expression level of this gene was observed as the TBT concentration in the culture media increased. No apparent differences were detected on the expression levels of sugE clone (sugE clone), suggesting that in this clone this gene is constitutively expressed. As expected, in clone pUC19 ...
31 Shareable Fragile X Facts (National Fragile X Foundation)
31 Shareable Fragile X Facts (National Fragile X Foundation)

... Fragile X Awareness month is here! We’ve put together a list of 31 facts that you can share each day of the week on sites like Facebook and Twitter. If you share them, please use the hashtags #FragileXFacts [2] and #KnowFragileX [3]! 1. Fragile X encompasses a group of conditions that can affect fam ...
Exercise During Pregnancy - University of Arkansas for Medical
Exercise During Pregnancy - University of Arkansas for Medical

... Complications of Exercise in Diabetic Pregnant Women - 2 • ACE inhibitors are contraindicated pregnancy secondary to risk of IUGR, fetal loss and neonatal hypotension • In late pregnancy, venous return and cardiac preload may be reduced after exertion leading to reduced stroke volume and reduced ca ...
A novel duplication in the HOXA13 gene in a family with atypical
A novel duplication in the HOXA13 gene in a family with atypical

... ventral side of the penis, is one of the most common congenital malformations with an incidence of 3 per 1000 males.1 Hypospadias is considered a complex trait caused by several genetic and environmental factors; low birth weight, for example, is associated with an increased risk for hypospadias.2–5 ...
Article A Distinct Mitochondrial Genome with DUI
Article A Distinct Mitochondrial Genome with DUI

... copies of species-specific mitochondrial genome. Mitochondrial DNA (mtDNA), albeit circular, doublestranded and short (&16.5 kb), is of crucial importance because genetic variation within the encoded sequence of mitochondrial protein subunits can have significant consequences for whole animal metabo ...
News from the west: Ancient DNA from a French megalithic burial
News from the west: Ancient DNA from a French megalithic burial

... isoamyl alcohol (25:24:1) organic extraction. Subsequently, the aqueous phase was concentrated in 100 lL of sterile distilled water with Centricon-30 columns (Amicon1) according to the manufacturer’s instructions. An extraction blank was systematically coextracted with the ancient Neolithic samples ...
bacterial plasmids - Acta Medica Medianae
bacterial plasmids - Acta Medica Medianae

... Plasmids contain genes essential for plasmid function and their preservation in a host cell (the beginning and the control of replication). Some of them possess genes which control plasmid stability. There is a common opinion that plasmids are unnecessary for a growth of bacterial population and the ...
1. True or False? The standard human karotype consists of 23 pairs
1. True or False? The standard human karotype consists of 23 pairs

Science and the Dead - Council for British Archaeology
Science and the Dead - Council for British Archaeology

... skeletons has traditionally relied upon measurement and visual examination of the remains. In recent years, scientific techniques involving destructive analysis of samples taken from bones or teeth have become increasingly important. The earliest of these laboratory analyses to find regular use in a ...
A GENETIC LINKAGE MAP OF Phycomyces blakesleeanus
A GENETIC LINKAGE MAP OF Phycomyces blakesleeanus

... A contig, short for “contiguous DNA”, is a set of overlapping DNA sequences generated during genome sequencing data assembly. LOD score Likelihood of odds scores Likelihood of odds scores are the probabilities of events occurring at that frequency by chance, expressed as the logarithm base 10. For e ...
Keratosis pilaris and ulerythema ophryogenes associated with an
Keratosis pilaris and ulerythema ophryogenes associated with an

... had a triangular face with coarse hair, high forehead, prominent ear helices with “floppy” earlobes, moderate bitemporal constriction, broad-based long nose with beaked nasal tip, short philtrum, wide mouth with protruding upper lip, high-arched palate, and abnormally shaped teeth. The neck, chest, ...
The Use of Cytochrome B Sequence Variation in Estimation of
The Use of Cytochrome B Sequence Variation in Estimation of

... In these studies, except those of Edwards and Wilson (1990), Johnsonand Cicero (199 l), Richman and Price (1992) and Lanyon (1992) mitochondrial variation was characterized through restriction fragment analysis. In the latter papers, cytochrome b sequence variation was used to estimate genetic varia ...
Information for couples where one partner carries
Information for couples where one partner carries

... How are haemoglobin E and haemoglobin Lepore inherited? They are inherited through genes. Every human characteristic, such as eye colour, or height, or type of haemoglobin is controlled by genes that we inherit from our parents. A child inherits two genes for every characteristic, one from each pare ...
Indications for caesarean section
Indications for caesarean section

... dystocia, 11% are performed for breech presentation and 10% are performed for fetal distress.2,3 In some South American countries section rates are said to be as high as 80%.4 Belizan et al5 demonstrated that the caesarean section rates are directly related to the Gross National Product per capita, ...
Genome-wide analysis by SNP Array
Genome-wide analysis by SNP Array

... Array) has revolutionised conventional cytogenetic diagnostics. This new technique analyses the whole genome with a higher resolution than that observed with classical karyotyping. Single nucleotide polymorphism (SNP) Array can detect and provide a detailed characterisation of the cryptic chromosoma ...
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Cell-free fetal DNA

Cell-free fetal DNA (cffDNA) is fetal DNA circulating freely in the maternal blood stream. It can be sampled by venipuncture on the mother. Analysis of cffDNA provides a method of non-invasive prenatal diagnosis.cffDNA originates from the trophoblasts making up the placenta. It is estimated that 2-6% of the DNA in the maternal blood is fetal in origin. The fetal DNA is fragmented and makes its way into the maternal bloodstream via shedding of the placental microparticles into the maternal bloodstream (figure 1). Studies have shown that cffDNA can first be observed as early as 7 weeks gestation, and the amount of cffDNA increases as the pregnancy progresses. cffDNA diminishes quickly after the birth of the baby, so that it is no longer detectable in the maternal blood approximately 2 hours after birth. cffDNA is significantly smaller than the maternal DNA in the bloodstream, with fragments approximately 200bp in size. Many protocols to extract the fetal DNA from the maternal plasma use its size to distinguish it from the maternal DNA.Studies have looked at, and some even optimized, protocols for testing non-compatible RhD factors, sex determination for X-linked genetic disorders and testing for single gene disorders. Current studies are now looking at determining aneuploidies in the developing fetus. These protocols can be done earlier than the current prenatal testing methods, and have no risk of spontaneous abortion, unlike current prenatal testing methods. Non-invasive prenatal diagnosis (NIPD) has been implemented in the UK and parts of the US; it has clear benefits above the standard tests of chorionic villi sample (CVS) and amniocentesis which have procedure-related miscarriage risks of about 1 in 100 pregnancies and 1 in 200 pregnancies, respectively.As a method of prenatal diagnosis, cell-free fetal DNA techniques share the same ethical and practical issues, such as the possibility of prenatal sex discernment and sex selection.
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