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HISTORY IN OB/GY
HISTORY IN OB/GY

... Date (year or how many years ago) and location of delivery Duration of gestation in weeks (if possible) Any important problems or complications during prenatal or antepartum course Duration of & type of labor (spontaneous or induced) Type of delivery (vaginal delivery, instrumental delivery such as ...
Test One Study Guide - Gordon State College
Test One Study Guide - Gordon State College

... • Down Syndrome: A chromosomally transmitted form of mental retardation caused by the presence of an extra chromosome (approximately 1 out of 700 live births). An individual with Down syndrome typically has a round face, a flattened skull, an extra fold of skin over the eyelids, a protruding tongue, ...
Genetic engineering in budding yeast
Genetic engineering in budding yeast

Physical Mapping I
Physical Mapping I

... An Example - Cystic Fibrosis • Cystic fibrosis is a fatal disease  1 in 25 Caucasians carries a faulty cystic fibrosis gene  Children who inherit faulty genes from both parents become sick  Best hope for a cure starts with finding the responsible gene  In the mid 80s nothing was known about the ...
Example Lab Report - UNC
Example Lab Report - UNC

... We preformed crosses between the variegated and wildtype plants. The F1 generation was scored for variegation with the result that variegated females mated with wildtype males gave rise to all variegated offspring while the reciprocal cross produced wildtype plants. This indicates that variegation i ...
Pregnancy
Pregnancy

...  As soon a it is born the baby breathes for the first time  The umbilical cord is tied and cut above the point where it attaches to the baby. ...
C2005/F2401 `09
C2005/F2401 `09

... implies they did find some synonymous mutations. B-1. Synonymous mutations were probably ignored because they are expected to change (the genotype only) (the phenotype only) (both) (neither) (beats me). B-2. Which of the following is/are ‘non-synonymous missense mutations? (See code on last page.) C ...
S4 File
S4 File

... 24 out of these 44 TNBC cases with the T panel. Blood vs. tumor germline concordance was higher but not significantly different in this series as compared to the BR series (mean [±SD] 89.12 [±12.97], 95% CI 85.29 – 92.95; min – max: 93 – 100). With the T panel, 27 out of 43 blood-only variants were ...
African Horse Sickness
African Horse Sickness

Genetica per Scienze Naturali aa 03
Genetica per Scienze Naturali aa 03

point mutations - Plant Developmental Biology
point mutations - Plant Developmental Biology

... Monoploid plants from tissue culture ...
Amplification and partial sequencing of Ixodes Scapularis Shaker
Amplification and partial sequencing of Ixodes Scapularis Shaker

... Although the elongase kit had been used, it had only been used twice within the entire experiment so the conditions had not been optimized for amplifying the tick and fruit fly DNA. The elongase kit had been designed to amplify templates up to 12 kb, so with the correct conditions, large fragments o ...
From Gene to Protein  I.
From Gene to Protein I.

... Each amino acid is joined to the correct tRNA by aminoacyl-tRNA synthetase. The 20 different synthetases match the 20 different amino acids. Each has active sites for only a specific tRNA-and-amino-acid combination. The synthetase catalyzes a covalent bond between them in a process driven by ATP hyd ...
Part Two - West Lakes GP Training
Part Two - West Lakes GP Training

... the GP to organise a kidney ultrasound for his two children aged 10 and 7, to see if they have inherited the condition. The GP should do so. ...
Chapter 04
Chapter 04

... 7.2.e Students know DNA (deoxyribonucleic acid) is the genetic material of living organisms and is located in the chromosomes of each cell. ...
Imprinted green beards: a little less than kin and more than kind
Imprinted green beards: a little less than kin and more than kind

... Figure 1. The mesiRNA ratchet. A population initially fixed for allele A (upper left) is successively invaded by an allele A 0 that also encodes a mesiRNA; an allele A8 that retains the mesiRNA but is insensitive to its effects and an allele A* that encodes a new mesiRNA (lower right). Subscripts m ...
Student`s guide -
Student`s guide -

... a large family, including people affected by the condition, have donated samples of their DNA to be tested. This DNA will enable you to discover how the condition is passed on and to predict whether individuals in the family will be directly affected by the condition. The relationships between the r ...
Horizontal Transfer of DNA From GM Crops to Bacteria and to
Horizontal Transfer of DNA From GM Crops to Bacteria and to

... transformation in some bacteria requires that at least 2 copies of a plasmid, or at least fragments of the 2nd copy, must be introduced simultaneously into the same bacterial cell so that overlapping segments are available to regenerate the plasmid in a natural transformation (Salyers 1998). Given t ...
Io mo0 - Journal of Medical Genetics
Io mo0 - Journal of Medical Genetics

... Shackleton et al.7 SSCP mobility shifts were detected in several sheep DNA samples (fig 1), and one SSCP was found to be the result of a guanine to adenine substitution at base pair position 1019 in the ovine CFTR cDNA sequence.6 This substitution destroys a MspI site, resulting in a 387 bp fragment ...
385 Genetic Transformation : a Retrospective
385 Genetic Transformation : a Retrospective

... (lower longer line) and donor chromosomal fragment (DNA molecule : upper shorter line), which could yield the observed transformants. The chromosomal regions marked -4are concerned with that part of the pathway of polysaccharide synthesis common to types I and I1 capsule ;those marked B determine th ...
Identification of Bacterial Species Using Colony PCR
Identification of Bacterial Species Using Colony PCR

... The reaction occurs in several steps to unwind and replicate the region of interest (Fig 1). The first step is denaturation. During this step, the reaction mixture is heated to 94-98°C, breaking the hydrogen bonds between complementary nucleotides and causing the two strands of DNA to separate. This ...
Revisedchapter12
Revisedchapter12

... codominant over i Of the three, each person carries two – leads to multiple blood types ...
Laboratory Projects
Laboratory Projects

... Cytoplasmic microtubule-based motor proteins contribute to the fidelity of chromosome repair ...
Lecture # 5 Mutations
Lecture # 5 Mutations

... If mutagens interact with DNA, they can produce mutations at high rates. Cells can sometimes repair the damage; but when they cannot, the DNA base ...
DNA helicase deficiencies associated with cancer
DNA helicase deficiencies associated with cancer

... Recent data indicate that BLM binds to the 70 kDa subunit of the heterotrimeric, single-stranded DNA binding protein, replication protein A (RPA) (46). This interaction stimulates the helicase activity of BLM (46). RPA is involved in DNA replication, repair and recombination, and can be detected on ...
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Cell-free fetal DNA

Cell-free fetal DNA (cffDNA) is fetal DNA circulating freely in the maternal blood stream. It can be sampled by venipuncture on the mother. Analysis of cffDNA provides a method of non-invasive prenatal diagnosis.cffDNA originates from the trophoblasts making up the placenta. It is estimated that 2-6% of the DNA in the maternal blood is fetal in origin. The fetal DNA is fragmented and makes its way into the maternal bloodstream via shedding of the placental microparticles into the maternal bloodstream (figure 1). Studies have shown that cffDNA can first be observed as early as 7 weeks gestation, and the amount of cffDNA increases as the pregnancy progresses. cffDNA diminishes quickly after the birth of the baby, so that it is no longer detectable in the maternal blood approximately 2 hours after birth. cffDNA is significantly smaller than the maternal DNA in the bloodstream, with fragments approximately 200bp in size. Many protocols to extract the fetal DNA from the maternal plasma use its size to distinguish it from the maternal DNA.Studies have looked at, and some even optimized, protocols for testing non-compatible RhD factors, sex determination for X-linked genetic disorders and testing for single gene disorders. Current studies are now looking at determining aneuploidies in the developing fetus. These protocols can be done earlier than the current prenatal testing methods, and have no risk of spontaneous abortion, unlike current prenatal testing methods. Non-invasive prenatal diagnosis (NIPD) has been implemented in the UK and parts of the US; it has clear benefits above the standard tests of chorionic villi sample (CVS) and amniocentesis which have procedure-related miscarriage risks of about 1 in 100 pregnancies and 1 in 200 pregnancies, respectively.As a method of prenatal diagnosis, cell-free fetal DNA techniques share the same ethical and practical issues, such as the possibility of prenatal sex discernment and sex selection.
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