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Page 1 MEIOSIS AND VARIATION A2.8 QUESTIONSHEET 1
Page 1 MEIOSIS AND VARIATION A2.8 QUESTIONSHEET 1

... in zygotene/early prophase of meiosis; chiasmata formation occurs in diakinesis/late prophase of meiosis; is cross over of genetic material between chromatids of homologous chromosomes; ...
Meiosis II
Meiosis II

... Usually cytokinesis occurs simultaneously with telophase I, forming two haploid daughter cells. Cleavage furrows form in animal cells and cell plates in plant cells. In some species, nuclear membranes and nucleoli reappear and the cell enters a time of interkinesis before meiosis II. In other specie ...
CHAPTER 6
CHAPTER 6

... 10.5 - What Are the Different Classes of Nucleic Acids? • DNA - one type, one purpose: - a single DNA molecules in virus and bacteria - Eukaryotic cells have many diploid chromosomes mainly in nucleus, but also mitochondria and chloroplasts. • RNA - 3 (or 4) types, 3 (or 4) purposes – ribosomal RNA ...
Individual eukaryotic genomes
Individual eukaryotic genomes

... The life cycle is extremely complex. Genome size: 22.8 Mb Chromosomes: 14 (range 0.6 to 3.3 Mb) Genes: 5268 (comparable to S. pombe)(1 gene/4300 bp) Website: http://www.plasmodb.org P. falciparum has an adenine+thymine (AT) content of 80.6%. The P. yoelli yoelli genome was also sequenced (infects ra ...
Introducing:
Introducing:

... gene map. It is called a map because it shows where the genes are located down the chromosome. Genes have numbers and letters that make up their names. •You can see how any rearrangement mutations in the chromosomes can alter the order and/or function of gene. •Numerical mutations will affect the nu ...
Specific biomolecules serve various functions in the body.
Specific biomolecules serve various functions in the body.

... 1. Study the statement above. Identify the molecule which contains the instructions used to create an organism’s enzymes and proteins. ...
Introduction to DNA Microarrays
Introduction to DNA Microarrays

... – Requires a new set of masks for each new array type Intro to gene chips - 4 ...
Gene transcription
Gene transcription

... Transkription Transkription ...
Understanding Our Environment
Understanding Our Environment

... enzymes to attach to the DNA.  Single-stranded RNA transcript produced. Chromosomes contain genes for building tRNA.  Each form of tRNA has a specific anticodon loop. - Base pairs with codon. Genes for rRNA also transcribed in the nucleus - Used to construct ribosomes. Stern - Introductory Plant B ...
IBC-Application-2017-Word - SUNY Downstate Office of Research
IBC-Application-2017-Word - SUNY Downstate Office of Research

... a. All persons conducting this work, including my collaborators, have received instruction on the specific hazards associated with the work and the specific safety equipment, practices, and behaviors required during the course of the work and use of these facilities. My records documenting this inst ...
DNA Prokaryote Transcription Steps (updated February 2013)
DNA Prokaryote Transcription Steps (updated February 2013)

... formation of the replication bubble. Once the RNA strand exceeds 10 nucleotides (bases), TFIIB drops off. Additional phosphorylation of the CTD of the RNA pol II by TFIIH pushes the polymerase into the elongation phase. TFIIH drops off. TFIID stays behind to form a new pre-initiation complex. TFIIF ...
The glpP and glpF genes of the glycerol regulon in
The glpP and glpF genes of the glycerol regulon in

... techniques. Plasmid DNA was prepared by the alkaline lysis method of Ish-Horowicz & Burke (198 1). When preparing plasmid DNA from B. subtilis, the cells were treated with lysozyme ( 5 g 1-') prior to lysis with NaOH/SDS. Plasmid DNA from E. coli XL1-Blue was prepared by the boiling method of Ausube ...
The Two Faces of Higher Eukaryotic DNA Replication Origins
The Two Faces of Higher Eukaryotic DNA Replication Origins

... replication origin contains an origin of bidirectional replication, as suggested by Burhans et al. (1990). We also propose, however, that replication does not initiate solely at this origin. Instead, initiation takes place throughout a large zone as proposed by Vaughn et al. (1990). Why a large zone ...
MICROBIAL GENETICS-III UGc - E
MICROBIAL GENETICS-III UGc - E

... phosphodiester bonds which link the 5’ carbon of the deoxyribose of one mononucleotide unit with the 3’ carbon of the deoxyribose of the next mononucleotide unit. According to Watson and Crick DNA molecule consists of two such polynucleotide chains wrapped helically around each other, with the sugar ...
CHAPTER 17 FROM GENE TO PROTEIN
CHAPTER 17 FROM GENE TO PROTEIN

... Transcription proceeds until after the RNA polymerase transcribes a terminator sequence in the DNA. ° In prokaryotes, RNA polymerase stops transcription right at the end of the terminator. ƒ Both the RNA and DNA are then released. ° In eukaryotes, the pre-mRNA is cleaved from the growing RNA chain w ...
Gene Section XPC (xeroderma pigmentosum, complementation group C) Atlas of Genetics and Cytogenetics
Gene Section XPC (xeroderma pigmentosum, complementation group C) Atlas of Genetics and Cytogenetics

... Repair (NER) repair capacity, but the residual repair has been shown to occur specifically in transcribed genes. It is very likely that the XPC-HR23B complex is the principal damage recognition complex i.e. essential for the recognition of DNA lesions in the genome. Binding of XPC-HR23B to a DNA les ...
Recombinant DNA WS
Recombinant DNA WS

... c. Which restriction enzyme is used to cut the GFP gene with? d. What sticky ends will the GFP have? Part D. Answer the questions regarding the Plasmid P. ...
Supplementary Methods
Supplementary Methods

... corresponding to the tetO operator (see below). The promotor of the GAL1 gene of S. cerevisiae was PCR amplified using Pfu DNA polymerase (Stratagene) and oligonucleotides gal320+ (5'-AAGAAGCTTGGAACTTCAGTAATACG-3') and gal725(5'-GAATAAGAAGTAATACAAACCGA-3'), digested with HindIII and cloned into the ...
Chromosome Microarray
Chromosome Microarray

... detected. Many of the current genetic research initiatives employ this array format,4 which benefits parallel development of clinical applications. The ultra high resolution is particularly important in the study of autism, where dosage changes may be very small and in the follow-up of developmental ...
Lesson 12: Single Trait Inheritance student notes
Lesson 12: Single Trait Inheritance student notes

... gain or loss of a sex chromosome. A single X chromosome leads to a female with Turner’s syndrome. XXY leads to Kleinfelter’s syndrome male. XYY is very uncommon, and leads to a male with relatively few differences from an XY male. It used to be believed that there was a disproportionately large prop ...
Microbial Discovery Activity - American Society for Microbiology
Microbial Discovery Activity - American Society for Microbiology

... (Do not choose an amino acid sequence if there are two identical ones.) 5. Ask the student responsible for that particular amino acid sequence for their sheet so that you can use their DNA sequence as the starting point for populating the next generation. 6. Repeat steps 2 through 5 as many times as ...
AP_Bio_power_point_lectures_files/Lecture Chpt. Themes
AP_Bio_power_point_lectures_files/Lecture Chpt. Themes

... •All life connected and traced back to prokaryotes ...
Current Awareness Of Issues Related To Genetically Modified Food
Current Awareness Of Issues Related To Genetically Modified Food

... A single chloroplast can contain between 30 and 200 copies of this genome, and a single mature photosynthetic leaf cell contains on average 50 chloroplasts. This equates to up to 500,000 chloroplasts and up to 1 million copies of the chloroplast genome per square cm of photosynthetic leaf tissue. Wh ...
DNA Self-assembly Model for Matrix Addition Problem
DNA Self-assembly Model for Matrix Addition Problem

... The first one is molecular recognition: elementary molecules selectively bind to others. The second is growth: elementary molecules or intermediate assemblies are the building blocks that bind to each other following a sequential or hierarchical assembly. The cooperativity and non-linear behavior of ...
Modified `one amino acid-one codon` engineering of high GC
Modified `one amino acid-one codon` engineering of high GC

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Extrachromosomal DNA



Extrachromosomal DNA is any DNA that is found outside of the nucleus of a cell. It is also referred to as extranuclear DNA or cytoplasmic DNA. Most DNA in an individual genome is found in chromosomes but DNA found outside of the nucleus also serves important biological functions.In prokaryotes, nonviral extrachromosomal DNA is primarily found in plasmids whereas in eukaryotes extrachromosomal DNA is primarily found in organelles. Mitochondrial DNA is a main source of this extrachromosomal DNA in eukaryotes. Extrachromosomal DNA is often used in research of replication because it is easy to identify and isolate.Extrachromosomal DNA was found to be structurally different from nuclear DNA. Cytoplasmic DNA is less methylated than DNA found within the nucleus. It was also confirmed that the sequences of cytoplasmic DNA was different from nuclear DNA in the same organism, showing that cytoplasmic DNAs are not simply fragments of nuclear DNA.In addition to DNA found outside of the nucleus in cells, infection of viral genomes also provides an example of extrachromosomal DNA.
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