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Determining the Structure of DNA
Determining the Structure of DNA

... 2) Watson and Crick believed that Pauling would soon recognise the error in his proposed DNA structure. How did Watson and Crick finally derive their structure. Watson and Crick’s rapport led them to speedy insights as well. They incessantly discussed the problem, bouncing ideas off one another. Thi ...
The Secret Code of Life: - Richmond School District
The Secret Code of Life: - Richmond School District

... nucleotides form a triplet which, when in a gene, codes for a part of a protein. There are 34 total different triplets that can be created but only 20 different amino acids. (Would a doublet code work just as well?? i.e. only 2 nucleotides to represent 20 amino acids. Why are there a lot of codes th ...
The iGEM Series
The iGEM Series

... Biochemical ‘scissors’ called restriction enzymes are used to cut the strings of DNA in different places and select the required genes. These genes are usually then inserted into circular pieces of DNA found in bacteria. The bacteria reproduce rapidly and within a short time thousands of identical c ...
Heredity Study Guide
Heredity Study Guide

... 3. __________ is the passing of traits from parents to offspring. 4. What trait appears in the first generation? 5. What trait appears in the second generation? 6. __________ are different forms of a characteristic. 7. What are alleles? 8. Instructions for an inherited trait are called _____. 9. Use ...
Information- Part 1 Study Guide
Information- Part 1 Study Guide

... (A) Viruses have highly efficient replicative capabilities that allow for rapid evolution and acquisition of new phenotypes. (B) Viruses replicate via a component assembly model allowing one virus to produce many progeny simultaneously via the lytic cycle. (C) Virus replication allows for mutations ...
Understanding DNA Technology
Understanding DNA Technology

... What is a Gene? A gene is a specific sequence of base pairs at a particular location on the chromosome. It codes for a specific product that generally has an effect on cell function. It is estimated there are between 22,000 and 28,000 genes in a beef or dairy animal. The genes are separated on the ...
DNA Technology
DNA Technology

... One gene of an insertion sequence codes for transposase, which catalyzes the transposon’s movement. The inverted repeats, about 20 to 40 nucleotide pairs long, are backward, upside-down versions of each other. In transposition, transposase molecules bind to the inverted repeats & catalyze the cuttin ...
Mutations
Mutations

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The Production of a

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Slide 1

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problem set #2
problem set #2

... b) The stage at which “sister chromatids go to opposite poles” immediately follows which of the above stages? c) Assuming that all nuclear DNA is restricted to chromosomes and that the amount of nuclear DNA essentially doubles during the S phase of interphase, how much nuclear DNA would be present ...
Unit 4 Review
Unit 4 Review

... them at least once. Revise your answers until all key terms are crossed out. Write in complete sentences. ...
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Chapter 16

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Evidence that a Safe Dose of Mutagen Does Not Exist
Evidence that a Safe Dose of Mutagen Does Not Exist

... steroids, etc. etc.) or alcohol a person may consume. Alcohol and most drugs upregulate bioactivation pathways and/or inhibit DNA repair pathways. Finally, no assay can take into account the extent to which detoxification enzymes and DNA repair enzymes are inactivated by mercury, lead, arsenic and o ...
Document
Document

... • Be able to describe the components of DNA electrophoresis, and recognize patterns in a gel • Be able to describe the form and function of restriction enzymes (restriction endonucleases) • Be able to describe the process of DNA-mediated transformation of bacterial cells • Discuss the molecular basi ...
Chapter 5-3 - Mahtomedi Middle School
Chapter 5-3 - Mahtomedi Middle School

Multiple choice questions BIO1130MM
Multiple choice questions BIO1130MM

... b. the time it takes to replicate the viral genome. c. the time it takes to assemble one viral particle. d. the period from infection to host cell lysis. MM.2 Viruses form a capsid around their nucleic acid core. What is this capsid composed of? X a. protein b. lipoprotein c. glycoprotein d. polysac ...
Chapter 8 Microbial Genetics
Chapter 8 Microbial Genetics

... • Sequence of AA – based on the sequence of mRNA – based on the NB sequence of the gene from which it was transcribed • Genetic information flows from the gene to mRNA to protein. • Change in the NB of the gene – change the codon on mRNA – change AA sequence of the protein – protein becomes ...
CH 9 cont
CH 9 cont

... These complementary bases were A bonded w/ ____ G bonded w/ _______ II. DNA REPLICATION What is it? __________ When does it occur? _____ Where does it occur ______? See p 286 and Draw ...
downloadable  file
downloadable file

... sequence. Next, you add a DNA priming sequence, the four nucleotides and an enzyme called DNA polymerase which incorporates new nucleotide bases making a new piece of DNA which is a copy of the original piece. In Sanger’s original method, four different sequencing reactions are performed. Each react ...
Gel Electrophoresis
Gel Electrophoresis

... molecules on the basis of their charge and size. The charged macromolecules migrate across a span of gel because they are placed in an electrical field. The gel acts as a sieve to to retard the passage of molecules according to their size and shape. ...
Human Mitochondrial DNA
Human Mitochondrial DNA

... more plasmids are “sucked” in. The culture is then quickly transferred to the ice which traps the plasmids within the cell’s membrane. Cells containing the foreign DNA grow and multiply within the tube, but to ensure that transformation was successful and purification of the gene of interest to proc ...
Join us in downtown Chicago, July 27-29, at the
Join us in downtown Chicago, July 27-29, at the

... As an added bonus for attending, we are offering optional DNAcreator v3 certification training for only $200! Combined with the early conference registration fee, this is $100 less than the normal v3 certification training…but with the all added learning and networking benefits of attending the DNAc ...
CHAPTER 1 - HCC Learning Web
CHAPTER 1 - HCC Learning Web

... • Biology consists of more than memorizing factual details • Themes help to organize biological information ...
Name AP EXAM REVIEW SESSION II ASSESSMENT QUIZ Use the
Name AP EXAM REVIEW SESSION II ASSESSMENT QUIZ Use the

... d. Sample 2 was cut at more restriction sites than was sample 4. e. Sample 4 was cut at more restriction sites than was sample 2. 6. Once a plasmid has incorporated specific genes, such as the gene coding for ampicillin resistance, the plasmid may be cloned by a. inserting it into a virus to generat ...
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Extrachromosomal DNA



Extrachromosomal DNA is any DNA that is found outside of the nucleus of a cell. It is also referred to as extranuclear DNA or cytoplasmic DNA. Most DNA in an individual genome is found in chromosomes but DNA found outside of the nucleus also serves important biological functions.In prokaryotes, nonviral extrachromosomal DNA is primarily found in plasmids whereas in eukaryotes extrachromosomal DNA is primarily found in organelles. Mitochondrial DNA is a main source of this extrachromosomal DNA in eukaryotes. Extrachromosomal DNA is often used in research of replication because it is easy to identify and isolate.Extrachromosomal DNA was found to be structurally different from nuclear DNA. Cytoplasmic DNA is less methylated than DNA found within the nucleus. It was also confirmed that the sequences of cytoplasmic DNA was different from nuclear DNA in the same organism, showing that cytoplasmic DNAs are not simply fragments of nuclear DNA.In addition to DNA found outside of the nucleus in cells, infection of viral genomes also provides an example of extrachromosomal DNA.
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