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Proving that DNA Replication is Semiconservative
Proving that DNA Replication is Semiconservative

... N-labeled DNA. Now that the parental DNA was labeled, Meselson and Stahl abruptly changed the medium to one containing 14N as the sole nitrogen source. From this point on, all the DNA synthesized by the bacteria would incorporate 14N, rather than 15N, so that the daughter DNA strands would contain o ...
A human laterality disorder associated with a homozygous
A human laterality disorder associated with a homozygous

... 552 amino acids protein, was not detected in cDNA of our control samples and exon 2 was skipped in the patient cDNA, whereas exon 3 was fully included, thus causing a frameshift. The possibility of a translation from an in-frame downstream ATG seems unlikely given that the next Met is only at residu ...
Recombinant DNA cloning technology
Recombinant DNA cloning technology

... plasmid) both be cut with the same enzyme (or with two enzymes which produce compatible ends). The insert DNA and the vector are then mixed, and DNA ligase is used to join the ...
Evolutionary Computation: A New Way to Search for Solutions
Evolutionary Computation: A New Way to Search for Solutions

... by a genetic code sequence • Rate each individual according to how well that creature solves the problem at hand • We can say that certain genetic code sequences are “better” at solving the problem than others • This is the basic idea behind Evolutionary Computation ...
Supplemental Material
Supplemental Material

... min., followed by gel analysis. D) 2D-gel analysis of replication intermediates from the rtf1-L162Y strain. The PstI-SacI fragment of plasmid pSC11 is analyzed. An analysis of plasmid pSC11 in the wild-type background is shown in Fig. 5C lower panel. E) The RTS1 sequence is required barrier activity ...
Section 6: Information Flow
Section 6: Information Flow

... processes of transcription and translation such that they have a solid foundation on which to build in the upcoming sections. To this end, we have provided a large number of active learning exercises from which instructors may choose. ...
GEP Annotation Report - GEP Community Server
GEP Annotation Report - GEP Community Server

... 2. For each Genscan prediction, perform a BLASTP search using the predicted amino acid sequence against the nr protein database using the strategy described above. 3. Examine the gene expression tracks (e.g., RNA-Seq) for evidence of transcribed regions that do not correspond to alignments to known ...
Large-Scale Variation Among Human and Great Ape Genomes
Large-Scale Variation Among Human and Great Ape Genomes

... large-scale sequence gain or loss events, defined here as copynumber altering events involving >10 kb of sequence, yet events that are undetectable at the cytogenetic level. Events of this size have the potential to significantly impact the gene complement and genome structure of closely related spe ...
GEP Annotation Report - GEP Community Server
GEP Annotation Report - GEP Community Server

... 2. For each Genscan prediction, perform a BLASTP search using the predicted amino acid sequence against the nr protein database using the strategy described above. ...
1. Which of the following statements about homologous
1. Which of the following statements about homologous

... Rhesus positive (Rh+ ) is dominant to Rhesus negative (Rh– ). A mother with Rhesus negative blood gives birth to a baby with Rhesus positive blood and there are concerns that subsequent pregnancies will trigger an immune response. What is a possible explanation for why subsequent pregnancies could t ...
PO Box 157
PO Box 157

... activation. Quantum information storage is now a recognized technology in the computer industry that involves optical and magneto-optical storage. Information storage in computers and in Aulterra’s products share a common mechanism, since they both require an external EM field to store the informati ...
Chapter 8: From DNA to Proteins
Chapter 8: From DNA to Proteins

...  A whole chromosome is too large for scientists to study a particular gene easily, so they had to find a way to get much smaller pieces of DNA.  In gel electrophoresis, an electrical current is used to separate a mixture of DNA fragments from each other.  A sample of DNA is loaded into a gel, whi ...
The Genetic Code
The Genetic Code

... The Central Dogma of Molecular Biology • Francis Crick proposed that DNA is an information storage molecule, and that the sequence of bases in DNA is a kind of code in which different combinations of bases could specify the 20 amino acids. • A particular stretch of DNA (a gene) contains the informa ...
The Genetic Code
The Genetic Code

... The Central Dogma of Molecular Biology • Francis Crick proposed that DNA is an information storage molecule, and that the sequence of bases in DNA is a kind of code in which different combinations of bases could specify the 20 amino acids. • A particular stretch of DNA (a gene) contains the informa ...
The Mitochondria as a Minimal Chassis:
The Mitochondria as a Minimal Chassis:

... “To allow homologous recombination between the new construct and rho+ mtDNA, the last S. douglasii cox1 exon and part of its terminator region were cloned upstream of the cox1::RIP1m gene. This large additional region homologous to the 3′ part of the cox1 gene (886 bp) should promote integration of ...
Practical Lecture 1
Practical Lecture 1

... - OBRC: Online Bioinformatics Resources Collection currently lists over 2826 databases (2013) ...
Transcription start sites
Transcription start sites

here - PHGEN
here - PHGEN

... sometimes structural or functional RNA. DNA that corresponds with a gene needs not be contiguous. The term mutation refers to a change in the primary DNA structure (DNA sequence, i.e. excess, absence or substitution of any of the nucleotides dA, dG, DT, or dC) producing a gene or a chromosome differ ...
GenIRL Genomic Information Retrieval using links
GenIRL Genomic Information Retrieval using links

... and different types of data. As biologists identify new genes and gene functions every day, new sequences are stored and new literature is published at an increasing speed. The size of nucleotide sequences databases such as GenBank is growing larger as well as the size of protein sequences, protein ...
Misconceptions, misunderstandings and questions students
Misconceptions, misunderstandings and questions students

... • Only mammalian life cycles contain meiosis, mitosis and fertilization Misunderstandings and questions: • Traits result from an organism’s DNA sequence • What is a flower? What is a fruit? State standards to be addressed by the intervention: GOAL 3 : Concepts of Biology The student will demonstrate ...
EDV- the Definition
EDV- the Definition

... a more accurate representation of the genome • Genome sequence is the genotype ...
Mutations - Kaikoura High School
Mutations - Kaikoura High School

... • If they occur in somatic cells then they are non-inheritable, if in gametes then can be passed on to offspring. • Can be due to mistakes in DNA replication (spontaneous) or caused by mutagenic agents e.g. UV light, ionising radiation, Xrays, chemicals, viruses ...
No Slide Title
No Slide Title

... of all genes, except those that reside on the sex chromosomes, there is a subset of these genes in which only the paternal or maternal copy is functional. One gene copy is silenced depending on the sex of the transmitting parent ...
A detailed gene map of pig chromosome 4, where the first
A detailed gene map of pig chromosome 4, where the first

... was found on chromosome 4 using a wild-boar intercross. Further mapping has refined the FAT1 QTL to a region conserved on both human chromosome 1 and 8. We have now improved the comparative map of the entire SSC4 by mapping 105 loci to pig chromosome 4 (SSC4) and defined the specific human chromosom ...
coding region of DNA. o Introns – non
coding region of DNA. o Introns – non

...  Activator proteins bind to enhancer regions further upstream either proximal (close by) or distal (many bp away).  Whether the STF are present or not determines if a given cell will initiate transcription or not.  They cause the DNA to fold as the specific transcription factor binds to the initi ...
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Genomics

Genomics is a discipline in genetics that applies recombinant DNA, DNA sequencing methods, and bioinformatics to sequence, assemble, and analyze the function and structure of genomes (the complete set of DNA within a single cell of an organism). Advances in genomics have triggered a revolution in discovery-based research to understand even the most complex biological systems such as the brain. The field includes efforts to determine the entire DNA sequence of organisms and fine-scale genetic mapping. The field also includes studies of intragenomic phenomena such as heterosis, epistasis, pleiotropy and other interactions between loci and alleles within the genome. In contrast, the investigation of the roles and functions of single genes is a primary focus of molecular biology or genetics and is a common topic of modern medical and biological research. Research of single genes does not fall into the definition of genomics unless the aim of this genetic, pathway, and functional information analysis is to elucidate its effect on, place in, and response to the entire genome's networks.
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