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Exam1 2012 Life Student
Exam1 2012 Life Student

... ____ 47. Most cells are small. When they reach a certain size, cells typically divide. This has to do with the a. amount of genetic material. b. surface-to-volume ratio. c. inability to produce more components of the cell membrane. d. fact that they cannot possess sufficient numbers of organelles fo ...
cell - immunology.unideb.hu
cell - immunology.unideb.hu

... ‘Non-self’ antigens/pathogens encounter the existing lymphocyte pool (repertoire) Antigens select their matching receptors from the available lymphocyte pool, induce clonal proliferation of specific clones and these clones differentiate to antibody secreting plasma cells The clonally distributed ant ...
Lambda Gene Family
Lambda Gene Family

... VJ or VDJ segments • These regions are extremely susceptible to mutation compared to “regular” DNA, about one base in 600 is altered per two generations of dividing (expanding) lymphocyte population ...
Transcription PPT
Transcription PPT

... suggested that genes coded for enzymes  each disease (phenotype) is caused by non-functional gene product ...
Human Identity Testing
Human Identity Testing

... that this is from the biological specimen at the crime scene. We now want to genotype Moe, Larry and Curly. We could do so and examine the electrophoretic bands as in Figure 11.2, but CODIS, as well as all other modern genotyping technologies, automates this procedure. Imagine a laser that is sensit ...
Projects
Projects

... Project 5. Optimizing vaccination scheduler for sexually transmitted diseases, and in particular for human papillomavirus (HPV) 16 and 18 infection. Supervisors: Andrei Korobeinikov and Tomas Alarcon (CRM), and Mireia Diaz Sanchis (Unit of Infections and Cancer, Institut Català d' Oncologia, IDIBELL ...
DNA & Protein Synthesis
DNA & Protein Synthesis

... Nucleotide sequence on one end which is known as the "Anitcodon" •This Anticodon sequence is complimentary to the Codon sequence found on the strand of mRNA •Each tRNA can bind specifically with a particular amino acid. ...
AP® BIOLOGY 2009 SCORING GUIDELINES (Form B)
AP® BIOLOGY 2009 SCORING GUIDELINES (Form B)

... Generate ends for attachment using endonucleases For joining of sticky ends Correct orientation of insertion to ensure expression DNA cut should be a complete sequence of gene Attach piece with a promoter or insert next to promoter Gene used to identify insertion of desired DNA Insert DNA with a gen ...
Table of Contents - Milan Area Schools
Table of Contents - Milan Area Schools

... Specific Defenses: The Immune System • Four characteristics of the immune system:  1. Specificity: Antigens are organisms or molecules that are specifically recognized by T cell receptors and antibodies.  The sites on antigens that the immune system recognizes are the antigenic determinants (or ep ...
File
File

... Viruses can mutate their DNA or RNA resulting in a slight change in viral structure – this can make them resistant to drugs and can prevent vaccinations from being effective; this is particularly true of viruses such as HIV. ...
2. Biotechnology
2. Biotechnology

... 65. You have a cDNA of unknown sequence in a plasmid vector. How could you do a PCR amplification of the cDNA without first determining its end sequences? 66. What aspects of PCR make it particularly useful in forensic investigations? How do the same properties make PCR particularly susceptible to c ...
Chapter 14 Study Workbook
Chapter 14 Study Workbook

... genomics, the study of whole genomes. The human genome project pinpointed genes and associated particular sequences in those genes with numerous diseases and disorders. It also found that the DNA of all humans matches base-for-base at most sites, but can vary at 3 million sites. The 1000 Genomes Pro ...
Recombination and Repair
Recombination and Repair

... fragments of DNA from their environment. In conjugation, one cell directly transfers genes (e.g., plasmid) to another cell. In transduction, viruses transfer genes between prokaryotes. ...
A Rapid Method for the Identification of Plasmid Desoxyribonucleic
A Rapid Method for the Identification of Plasmid Desoxyribonucleic

... Currently two types of rapid screening techniques for plasmid desoxyribonucleic acid (DNA) are used (1,4,5,7). One type requires little starting material, but subjects the DNA to considerable stress during lysis (5,7) or during separation of plasmid DNA from chromosomal DNA (1) and is therefore not ...
Bacterial Transformation - Baldwinsville Central School
Bacterial Transformation - Baldwinsville Central School

... charge of DNA phosphates & helps neutralize cell membrane so plasmid can get in 2. Incubate on ice -Slows movement of cell membrane so Ca++ can bind & plasmid can slip into bacterial cell ...
pGLO
pGLO

... charge of DNA phosphates & helps neutralize cell membrane so plasmid can get in 2. Incubate on ice -Slows movement of cell membrane so Ca++ can bind & plasmid can slip into bacterial cell ...
Characterisation of DNA by Agarose Gel Electrophoresis and
Characterisation of DNA by Agarose Gel Electrophoresis and

... nucleus. Additionally eukaryotic DNA is associated with basic proteins called histones. They take an important role in causing the high condensation of DNA in its chromosomal form for transport during cell division. Besides the DNA in the nucleus extrachromosomal DNA is found in mitochondria and pla ...
Chapter 17
Chapter 17

...  Immediate protection against recent infection or ongoing disease  Antisera have several limitations ○ Contain antibodies against many antigens ...
Word version
Word version

... 1. Active immunity Active immunity occurs when the animal produces antibodies either in response to natural challenges from disease, or as a result of vaccination. Once an animal has produced a specific antibody, it can produce more antibodies of the same type rapidly in response to an infection. Th ...
Simple and chemical DNA extraction from preserved bivalve mantle
Simple and chemical DNA extraction from preserved bivalve mantle

... In our experiments, however, by using the extract obtained from the step [1] with neither subsequent ethanol precipitation nor silica treatment, no reproducible amplification was detected (Figure 1). Presumably, this was due to significant amounts of protein and other cell materials present in the m ...
Student factsheet for this topic
Student factsheet for this topic

CH 16-17: DNA, RNA & PROTEINS
CH 16-17: DNA, RNA & PROTEINS

... cooperation with other associated factors to help control gene expression. The number and type of SRF-associated factors determines which genes are expressed, where they are expressed, and when they are expressed. SRF and the other factors bind a DNA sequence known as the Serum Response Element (SRE ...
Eukaryotic Gene Regulation
Eukaryotic Gene Regulation

... – Are tissue specific and temporoal-specific – E.g. found in gene that produces a hormone involved in thyroid production/stimulation . This hormone is only produced in pituitary cells. Expression only occurs in these cells because of a silencer that binds a cellular factor which repress transcriptio ...
polymerase chain reaction
polymerase chain reaction

... There is the fear that crops carrying genes from other species are a health concern and could do ecological harm.  So a transgenic plant could transfer the new genes to a closely related species that was never intended to be modified. So if a weeds we want to control picked up a gene from a modifie ...
Ch. 24 Presentation
Ch. 24 Presentation

...  Vertebrate innate immunity includes – barriers such as skin and mucous membranes, – interferons, proteins produced by virus-infected cells, that help to limit the cell-to-cell spread of viruses, ...
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DNA vaccination



DNA vaccination is a technique for protecting an animal against disease by injecting it with genetically engineered DNA so cells directly produce an antigen, resulting in a protective immunological response. Several DNA vaccines have been released for veterinary use, and there has been promising research using the vaccines for viral, bacterial and parasitic diseases, as well as to several tumour types. Although only one DNA vaccine has been approved for human use, DNA vaccines may have a number of potential advantages over conventional vaccines, including the ability to induce a wider range of immune response types.
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