Construction of a new cloning vector utilizing a cryptic plasmid and
... plasmid, pHY6202. We constructed a Streptomyces cloning vector, pSY10CMM, consisting of the HUT6202 rep gene, the thiostrepton resistance gene and an operon encoding the synthesis of melanin pigment, abbreviated mel, from S. castaneoglobisporus. The vector, which has SphI and BamHI sites as cloning ...
... plasmid, pHY6202. We constructed a Streptomyces cloning vector, pSY10CMM, consisting of the HUT6202 rep gene, the thiostrepton resistance gene and an operon encoding the synthesis of melanin pigment, abbreviated mel, from S. castaneoglobisporus. The vector, which has SphI and BamHI sites as cloning ...
Defenses Against Infection
... Antigens How does the immune system recognize “others”? Specific immune defenses are triggered by molecules called antigens. An antigen is any foreign substance that can stimulate an immune response. Typically, antigens are located on the outer surfaces of bacteria, viruses, or parasites. The immune ...
... Antigens How does the immune system recognize “others”? Specific immune defenses are triggered by molecules called antigens. An antigen is any foreign substance that can stimulate an immune response. Typically, antigens are located on the outer surfaces of bacteria, viruses, or parasites. The immune ...
Untitled
... alternative protocol developed by US researchers. Fred’s method revolutionised genetics over the following three decades, and has only recently been superseded by modern ‘next-generation’ technology – the super-speedy high-definition Blu-ray disc to replace Fred’s clunky VHS cassette. Most of us wil ...
... alternative protocol developed by US researchers. Fred’s method revolutionised genetics over the following three decades, and has only recently been superseded by modern ‘next-generation’ technology – the super-speedy high-definition Blu-ray disc to replace Fred’s clunky VHS cassette. Most of us wil ...
Lyme Borreliosis is a complex multisystem illness caused by
... via endocytic pits, and induce death via membrane permeabilization of both B and T lymphocytes, with B lymphocytes showing apoptosis to a greater degree (Dorward et al 1997). In contrasting results to the Sandra study, no cytotoxicity was observed from heat-killed Bb or Bb sonicates. In a separate p ...
... via endocytic pits, and induce death via membrane permeabilization of both B and T lymphocytes, with B lymphocytes showing apoptosis to a greater degree (Dorward et al 1997). In contrasting results to the Sandra study, no cytotoxicity was observed from heat-killed Bb or Bb sonicates. In a separate p ...
Slides
... for a gene product, protein, or RNA §Genome - complete DNA base sequence of an organism §Replication - DNA synthesis involves complementary base pairing between the parental and newly synthesized strand ...
... for a gene product, protein, or RNA §Genome - complete DNA base sequence of an organism §Replication - DNA synthesis involves complementary base pairing between the parental and newly synthesized strand ...
PPT - IAS-USA
... • Infusion of ZFN-treated ex vivo expanded autologous Tcells is generally safe and well tolerated • Durable increases seen in both CD4 counts and total Tcell counts (for CD4+CD8 infusions) • CCR5 modified T-cells persist long-term in vivo • Cytoxan conditioning further improves both total T-cell cou ...
... • Infusion of ZFN-treated ex vivo expanded autologous Tcells is generally safe and well tolerated • Durable increases seen in both CD4 counts and total Tcell counts (for CD4+CD8 infusions) • CCR5 modified T-cells persist long-term in vivo • Cytoxan conditioning further improves both total T-cell cou ...
Antibody structure and isotypes
... F(ab) and Fc regions The Y-shape of an antibody can be divided into three sections: two F(ab) regions and an Fc region. The F(ab) regions contain the variable domain that binds to cognate antigens. The Fc fragment provides a binding site for endogenous Fc receptors on the surface of lymphocytes, and ...
... F(ab) and Fc regions The Y-shape of an antibody can be divided into three sections: two F(ab) regions and an Fc region. The F(ab) regions contain the variable domain that binds to cognate antigens. The Fc fragment provides a binding site for endogenous Fc receptors on the surface of lymphocytes, and ...
Supplemental material
... each representing the paired sister centromeres of homologous third chromosomes, are visible within one bivalent at prometaphase I (PMI). The two foci segregate to opposite spindle poles at anaphase I (AI). Sister foci remain fused, indicating that sister centromeres are held together during prometa ...
... each representing the paired sister centromeres of homologous third chromosomes, are visible within one bivalent at prometaphase I (PMI). The two foci segregate to opposite spindle poles at anaphase I (AI). Sister foci remain fused, indicating that sister centromeres are held together during prometa ...
Rapid and simple method for DNA extraction from plant and algal
... The PCR is the most frequently used technique for amplification of specific DNA fragments in vitro. Because of its high sensitivity, PCR does not require high-quality template DNA obtained using the complicated conventional method with proteinase K, phenol, and chloroform. Numerous shortcuts have be ...
... The PCR is the most frequently used technique for amplification of specific DNA fragments in vitro. Because of its high sensitivity, PCR does not require high-quality template DNA obtained using the complicated conventional method with proteinase K, phenol, and chloroform. Numerous shortcuts have be ...
CHAPTER 6
... (G for gap, not growth) is typically the longest part of the cell cycle; G1, is characterized by rapid growth and metabolic activity. Cells that are quiescent, that is, not growing and dividing (such as neurons), are said to be in G0. The S phase is the time of DNA synthesis. S is followed by G2, a ...
... (G for gap, not growth) is typically the longest part of the cell cycle; G1, is characterized by rapid growth and metabolic activity. Cells that are quiescent, that is, not growing and dividing (such as neurons), are said to be in G0. The S phase is the time of DNA synthesis. S is followed by G2, a ...
The Human Gene AHNAK Encodes a Large Phosphoprotein
... The initial characterization of the sera was done using portions of AHNAK protein expressed in Escherichia coli. The cDNA fragments of AHNAK were cloned into appropriate pGEX vectors (11) to produce fusion proteins of glutathionS-transferase (GST) 1 and AHNAK-specific polypeptides. Extracts from bac ...
... The initial characterization of the sera was done using portions of AHNAK protein expressed in Escherichia coli. The cDNA fragments of AHNAK were cloned into appropriate pGEX vectors (11) to produce fusion proteins of glutathionS-transferase (GST) 1 and AHNAK-specific polypeptides. Extracts from bac ...
Upwelling, Downwelling, and El Nino
... Transformation: assimilation of external genetic material by a cell Not a protein-heat denatures proteins but heat did not destroy the transforming ability of the genetic material in the heat killed S cells Later Avery, McCarty, and MacLeod discovered transforming agent was DNA ...
... Transformation: assimilation of external genetic material by a cell Not a protein-heat denatures proteins but heat did not destroy the transforming ability of the genetic material in the heat killed S cells Later Avery, McCarty, and MacLeod discovered transforming agent was DNA ...
Immune Response in Infections Caused by Helminthes
... spp. intraluminal presence of Ascaris spp. in the intestine) (7-11). Intermitent licking of the fluid from echinoccocous cyst can lead to the transient eosinophilia associated with hypersensitive reaction type 1 (anafilactic reaction). Prolonged hypereosinophilia is present in strongiloidosis, filar ...
... spp. intraluminal presence of Ascaris spp. in the intestine) (7-11). Intermitent licking of the fluid from echinoccocous cyst can lead to the transient eosinophilia associated with hypersensitive reaction type 1 (anafilactic reaction). Prolonged hypereosinophilia is present in strongiloidosis, filar ...
Clearance mechanism of a mannosylated antibody–enzyme fusion
... yeast Pichia pastoris and produced to clinical grade. The P. pastoris-derived mannosylation of the fusion protein aids rapid normal tissue clearance required for successful ADEPT. The work presented provides evidence that MFECP1 is cleared by the endocytic and phagocytic mannose receptor (MR), which ...
... yeast Pichia pastoris and produced to clinical grade. The P. pastoris-derived mannosylation of the fusion protein aids rapid normal tissue clearance required for successful ADEPT. The work presented provides evidence that MFECP1 is cleared by the endocytic and phagocytic mannose receptor (MR), which ...
The Human Gene AHNAK Encodes a Large
... The initial characterization of the sera was done using portions of AHNAK protein expressed in Escherichia coli. The cDNA fragments of AHNAK were cloned into appropriate pGEX vectors (11) to produce fusion proteins of glutathionS-transferase (GST) 1 and AHNAK-specific polypeptides. Extracts from bac ...
... The initial characterization of the sera was done using portions of AHNAK protein expressed in Escherichia coli. The cDNA fragments of AHNAK were cloned into appropriate pGEX vectors (11) to produce fusion proteins of glutathionS-transferase (GST) 1 and AHNAK-specific polypeptides. Extracts from bac ...
The Immune System - Body Defenses
... extracellular fluids (blood plasma, lymph, mucus, etc.) and the surface of B cells. Defense against bacteria, bacterial toxins, and viruses that circulate freely in body fluids, before they enter cells. Also cause certain reactions against transplanted tissue. Antigenic determinants - specific regio ...
... extracellular fluids (blood plasma, lymph, mucus, etc.) and the surface of B cells. Defense against bacteria, bacterial toxins, and viruses that circulate freely in body fluids, before they enter cells. Also cause certain reactions against transplanted tissue. Antigenic determinants - specific regio ...
Leukaemia Section t(11;19)(q23;p13.1) Atlas of Genetics and Cytogenetics in Oncology and Haematology
... Phenotype / cell stem origin M4/M5 most often; M1/M2 at times; therapy related AL; however, clonal rearrangements of IgH gene have been found, demonstrating a biphenotypic nature. Epidemiology Children and, most often, adults (7 days to 83 yrs); congenital cases are rare, in contrast with the t(11;1 ...
... Phenotype / cell stem origin M4/M5 most often; M1/M2 at times; therapy related AL; however, clonal rearrangements of IgH gene have been found, demonstrating a biphenotypic nature. Epidemiology Children and, most often, adults (7 days to 83 yrs); congenital cases are rare, in contrast with the t(11;1 ...
LEARNING OBJECTIVE 1: Describe the general functions of the
... of Medicine, vol. 332. A small percentage of people infected with HIV for more than a decade have not become ill—why? This issue of the journal contains several articles on long-term survivors of AIDS. Diamond, Jared. September 1992. The mysterious origin of AIDS. Natural History. Did the AIDS epide ...
... of Medicine, vol. 332. A small percentage of people infected with HIV for more than a decade have not become ill—why? This issue of the journal contains several articles on long-term survivors of AIDS. Diamond, Jared. September 1992. The mysterious origin of AIDS. Natural History. Did the AIDS epide ...
IMMUNOLOGIC DEFICIENCY SYNDROMES
... Immature APC cells: Low MHC Class I & II and CD80/86 (B7.1/7.2) co-stimulatory molecules Mature APC cells: Increased MHC & CD80/86 T-cells also express inhibitory B7 receptor, CTLA-4 ((-/-) mice assoc. with AI disease) Polymorphisms assoc. with human AI disease ...
... Immature APC cells: Low MHC Class I & II and CD80/86 (B7.1/7.2) co-stimulatory molecules Mature APC cells: Increased MHC & CD80/86 T-cells also express inhibitory B7 receptor, CTLA-4 ((-/-) mice assoc. with AI disease) Polymorphisms assoc. with human AI disease ...
EXPRESSED SEQUENCE TAGS FROM IMMUNE TISSUES OF
... (SNPs)) are provided. This information enabled us to obtain a preliminary view of regulated genes in response to these pathogens and it constitutes the basis for subsequent and more accurate microarray analysis. An important factor that determines the usefulness of generated data for biologists is e ...
... (SNPs)) are provided. This information enabled us to obtain a preliminary view of regulated genes in response to these pathogens and it constitutes the basis for subsequent and more accurate microarray analysis. An important factor that determines the usefulness of generated data for biologists is e ...
T-CELL RECEPTOR (TCR) PLAY AN IMPORTANT ROLE
... (ALTHOUGH OTHER COMPONENTS MAY CONTRIBUTE TO THESE CHANGES IN SENSITIVITY) ...
... (ALTHOUGH OTHER COMPONENTS MAY CONTRIBUTE TO THESE CHANGES IN SENSITIVITY) ...
Mutations - GK-12 Program at the University of Houston
... NGSS, Life Sciences, High School (6-8), HS-LS3-2, Make and defend a claim based on evidence that inheritable genetic variations may result from: (1) new genetic combinations through meiosis, (2) viable errors occurring during replication, and/or (3) mutations caused by environmental factors. Pre-Req ...
... NGSS, Life Sciences, High School (6-8), HS-LS3-2, Make and defend a claim based on evidence that inheritable genetic variations may result from: (1) new genetic combinations through meiosis, (2) viable errors occurring during replication, and/or (3) mutations caused by environmental factors. Pre-Req ...
Chapter 16: Lymphatic System and Immunity
... 9. A clone is a cell that is identical to the cell from which it was derived. 10. Different varieties of T cells and B cells have a particular type of antigen receptor on their cell membranes that can respond only to a specific antigen. E. T Cells and the Cellular Immune Response ...
... 9. A clone is a cell that is identical to the cell from which it was derived. 10. Different varieties of T cells and B cells have a particular type of antigen receptor on their cell membranes that can respond only to a specific antigen. E. T Cells and the Cellular Immune Response ...
DNA vaccination
DNA vaccination is a technique for protecting an animal against disease by injecting it with genetically engineered DNA so cells directly produce an antigen, resulting in a protective immunological response. Several DNA vaccines have been released for veterinary use, and there has been promising research using the vaccines for viral, bacterial and parasitic diseases, as well as to several tumour types. Although only one DNA vaccine has been approved for human use, DNA vaccines may have a number of potential advantages over conventional vaccines, including the ability to induce a wider range of immune response types.