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UCSC Known Genes (by Jim Kent)
UCSC Known Genes (by Jim Kent)

... • Weight of 3 on an edge is good enough. • Single exon gene edges take 4 though. • Rank input RNA by whether refSeq, and number of good edges they use. • If any good edges, output a transcript consisting of the edges used by the first RNA. • Output transcript based on next RNA if the good edges it u ...
DNA and RNA
DNA and RNA

... Before discussing the major role of DNA, it is important to discuss DNA’s first cousin, ribonucleic acid or RNA. Besides its chemical composition, RNA has important similarities and differences with DNA. First, like DNA, RNA has four and only four nucleotides. But unlike DNA, RNA uses the nucleotide ...
M2 RNA Pol Ⅰ genes
M2 RNA Pol Ⅰ genes

... D Enhancers typically lie 100-200 bp upstream from the start of transcription. 6. Which one of the following statements about general transcription factors is false? A TFIID binds to the T ATA box. B TFIID is a multi protein complex consisting of TBP and TAFIIs. C TBP is a common factor in transcrip ...
lec-02-transcript
lec-02-transcript

... phosphate group. The sugar and base are linked to form a nucleoside and attachment of the phosphate group results in a nucleotide. Many such nucleotide units are linked together by means of a covalent bond known as the phosphodiester bond. This is formed between the 3' carbon of one sugar and 5' car ...
Document
Document

... • The polypeptide chain is released from the ribosome and becomes an active protein. Sometimes several ribosomes (polysome) translate the same strand of mRNA at the same time to produce several peptide chains. ...
Document
Document

... CC sequence. For shRNA rescue experiments a plasmid containing GFP-RanBP2 [5], was used. In addition a mutant version lacking zinc fingers 3-8, which lacks 4371-5694 nucleotides of the human RanBP2 ORF, was constructed by digesting the GFP-tagged full length human RanBP2 with SwaI restriction enzyme ...
Dear students, Under Boston`s asking, I persude the leader to agree
Dear students, Under Boston`s asking, I persude the leader to agree

... A. It progresses in both directions away from each point of origin on the chromosome B. It requires a DNA template that is copied in its 5' to 3' direction C. It occurs during the M phase of the cell cycle D. It produces one newly synthesized double helix and one composed of the two parental strands ...
Teacher shi 18940209087 Email:  QQ
Teacher shi 18940209087 Email: QQ

... A. It progresses in both directions away from each point of origin on the chromosome B. It requires a DNA template that is copied in its 5' to 3' direction C. It occurs during the M phase of the cell cycle D. It produces one newly synthesized double helix and one composed of the two parental strands ...
Analysis of the 3′-terminal nucleotide sequence of vesicular
Analysis of the 3′-terminal nucleotide sequence of vesicular

... tract at the 3'-terminus of the mRNA encoding the N polypeptide of vesicular stomatitis virus has been determined by copying with reverse transcriptase and using 2',3'-dideoxynucleoside triphosphates as specific chain terminators. The method appears highly suitable for sequence determination in any ...
HL Protein Synthesis Question Sheet
HL Protein Synthesis Question Sheet

... ribosomes. Because of this, mRNA molecules must be short so that they can travel through the nuclear pores. Once in the cytoplasm, mRNA molecules can become degraded and broken down. In eukaryotes the mRNA molecules are modified while still in the nucleus to prevent this. The average lifespan of a e ...
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Chapter 10

... In prokaryotes, tRNA can be found either as single genes or as parts of operons that can also contain combinations of mRNAs or rRNAs. In any case, whether from a single gene, or after the initial cleavage to separate the tRNA transcript from the rest of the transcript, the resulting pre-tRNA has an ...
SAM Teachers Guide - RI
SAM Teachers Guide - RI

... Students explore the structure and function of two of the four major macromolecules:  proteins and nucleic acids. On the first day they explore proteins and on the second  day, the nucleic acids making up DNA and RNA. After examining the atomic structure  of proteins, students consider linear polyme ...
The Never-Ending Story—The Origin and Diversification of Life
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... reacts with hydrogen sulfide (H2S), a pyrite crystal (FeS2), or “fool’s gold,” forms as well as hydrogen gas, releasing energy in the process. Feedback loops can develop when two molecules regulate their respective synthesis. Since the formation of pyrite is an exergonic reaction, it has the potenti ...
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Chapter 2 DNA to end Multiple Choice

... A. Enzymes contain the code for DNA. B. Enzymes act on DNA during translation. C. Both enzymes and DNA have similar shapes. D. The structure of enzymes is determined by DNA. ...
BIS2A TM Murphy Page 1 PROBLEMS ON MOLECULAR BIOLOGY
BIS2A TM Murphy Page 1 PROBLEMS ON MOLECULAR BIOLOGY

... chains coded by the two messengers. Why don’t different messages always give different polypeptides? c). List the differences in base sequences between mRNA 1 and mRNA 3. List the differences in amino acid sequences in the polypeptide chains coded by the two messengers. What is the relationship betw ...
chapter9_Sections 4-6 - (per 3) and wed 4/24 (per 2,6)
chapter9_Sections 4-6 - (per 3) and wed 4/24 (per 2,6)

... Codons and Amino Acids • There are only twenty kinds of amino acids found in proteins, so some amino acids are specified by more than one codon • Some codons signal the beginning and end of a proteincoding sequence: • AUG (methionine) start translation • UAA, UAG, and UGA are stop codons • The orde ...
Genetics Journal Club
Genetics Journal Club

... mechanisms of obesity by looking at coding and non-coding RNA expression patterns in patients with PWS, ALMS, non-syndromic obesity and comparing them to non-obese subjects. -Disease specific patterns ...
2.4 RNA and Protein Synthesis
2.4 RNA and Protein Synthesis

... –Genetic code – universal language of genetics used by virtually all living organisms •Works in three nucleotide units of mRNA called codons •Each codon codes for a single amino acid •One amino acid may be coded for by more than one codon –There are 64 codons and only 20 amino acids – degeneracy of ...
Lecture 7 - Columbus Labs
Lecture 7 - Columbus Labs

... complex triggers Cleavage of the polypeptide chain from the tRNA and then the dissociation of the ribosome complex ...
6.3 Translation: Synthesizing Proteins from mRNA
6.3 Translation: Synthesizing Proteins from mRNA

... growing polypeptide chain, a peptide bond is formed and the entire chain resides on the A sire of the large ribosomal subunit. ...
Brooker Chapter 12
Brooker Chapter 12

... the sequence of nucleotides in the mRNA This in turn corresponds to the sequence of amino acid in the polypeptide ...
MicroRNAs
MicroRNAs

... Often in clusters. Many times near the genes they regulate or inside them. ...
ppt for
ppt for

... hybridization. Standard sequencing runs could take place on channels with a 127.5 mm2 surface area, requiring 2,750 images to be taken per cycle to image the entire channel area. The surface area needed to accommodate ~350,000 mRNA molecules contained in a single cell is ~0.4 mm2; thus, only eight i ...
BIS2A TM Murphy Page 1 PROBLEMS ON MOLECULAR BIOLOGY
BIS2A TM Murphy Page 1 PROBLEMS ON MOLECULAR BIOLOGY

... chains coded by the two messengers. Why don’t different messages always give different polypeptides? c). List the differences in base sequences between mRNA 1 and mRNA 3. List the differences in amino acid sequences in the polypeptide chains coded by the two messengers. What is the relationship betw ...
Translation
Translation

... the acid group of one amino acid is linked to  the amino group of the next amino acid, forming a peptide bond. ...
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RNA



Ribonucleic acid (RNA) is a polymeric molecule implicated in various biological roles in coding, decoding, regulation, and expression of genes. RNA and DNA are nucleic acids, and, along with proteins and carbohydrates, constitute the three major macromolecules essential for all known forms of life. Like DNA, RNA is assembled as a chain of nucleotides, but unlike DNA it is more often found in nature as a single-strand folded onto itself, rather than a paired double-strand. Cellular organisms use messenger RNA (mRNA) to convey genetic information (using the letters G, U, A, and C to denote the nitrogenous bases guanine, uracil, adenine, and cytosine) that directs synthesis of specific proteins. Many viruses encode their genetic information using an RNA genome.Some RNA molecules play an active role within cells by catalyzing biological reactions, controlling gene expression, or sensing and communicating responses to cellular signals. One of these active processes is protein synthesis, a universal function whereby mRNA molecules direct the assembly of proteins on ribosomes. This process uses transfer RNA (tRNA) molecules to deliver amino acids to the ribosome, where ribosomal RNA (rRNA) links amino acids together to form proteins.
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