Slide 1
... cell survival under nonselective conditions. Episome no longer in use. They usually occur in bacteria, sometimes in eukaryotic organisms (e.g., the 2um-ring in yeast S. cerevisiae). Sizes: 1 to over 400 kb. Copy numbers: 1 - hundreds in a single cell, or even thousands of copies. Every plasmid conta ...
... cell survival under nonselective conditions. Episome no longer in use. They usually occur in bacteria, sometimes in eukaryotic organisms (e.g., the 2um-ring in yeast S. cerevisiae). Sizes: 1 to over 400 kb. Copy numbers: 1 - hundreds in a single cell, or even thousands of copies. Every plasmid conta ...
• Transcription Transcription • Translation Information flow in
... How similar are the promoter core elements (-10,-35, and their spacing) to the consensus? - in general the more similar they are, the more active the promoter will be to initiate transcription - however, some positions are more important than others ...
... How similar are the promoter core elements (-10,-35, and their spacing) to the consensus? - in general the more similar they are, the more active the promoter will be to initiate transcription - however, some positions are more important than others ...
BDOL Interactive Chalkboard
... Sequencing the human genome • The difficult job of sequencing the human genome is begun by ____________ samples of DNA into fragments using restriction enzymes. • Then, each individual fragment is cloned and sequenced. The cloned fragments are aligned in the ______ order by overlapping matching seq ...
... Sequencing the human genome • The difficult job of sequencing the human genome is begun by ____________ samples of DNA into fragments using restriction enzymes. • Then, each individual fragment is cloned and sequenced. The cloned fragments are aligned in the ______ order by overlapping matching seq ...
Soybean (Glycine max L.) Nuclear DNA Contains
... transcribed tufA genes exist. Equivalent fragments were cut from both cDN A samples (cDNAl and cDNA2) and used as probes in Southern experiments (Fig. 1). We see that either probe interacts with four HindIII fragments albeit in adifferential manner: cDNA2 (panel I) gives a strong signal with the two ...
... transcribed tufA genes exist. Equivalent fragments were cut from both cDN A samples (cDNAl and cDNA2) and used as probes in Southern experiments (Fig. 1). We see that either probe interacts with four HindIII fragments albeit in adifferential manner: cDNA2 (panel I) gives a strong signal with the two ...
Chap2 DNA RNA and Protein
... In mammals, the methylation of DNA occurs at the cytosine bases in CpG dinucleotide via the methyltransferase [1]. A high CpG content is found in regions known as CpG islands (a stretch of DNA 1-2 kb that has clusters of CpG doublets). CpG islands surround the promoters of constitutively expressed ...
... In mammals, the methylation of DNA occurs at the cytosine bases in CpG dinucleotide via the methyltransferase [1]. A high CpG content is found in regions known as CpG islands (a stretch of DNA 1-2 kb that has clusters of CpG doublets). CpG islands surround the promoters of constitutively expressed ...
Chapter 3
... that bears in its nucleotide sequence the linear array of genes. In prokaryotes, chromosomal DNA is circular, and the entire genome is carried on one chromosome. Eukaryotic genomes consist of a number of chromosomes whose DNA is associated with different kinds of proteins. daughter cells – two cells ...
... that bears in its nucleotide sequence the linear array of genes. In prokaryotes, chromosomal DNA is circular, and the entire genome is carried on one chromosome. Eukaryotic genomes consist of a number of chromosomes whose DNA is associated with different kinds of proteins. daughter cells – two cells ...
DNA and Forensic Science
... Now that it is clear how important DNA is to generating and maintaining life, we will discuss the application of DNA to forensic testing. As discussed, DNA is composed of a string of nucleotides. Within each human cell, this string is approximately three billion bases long. A fortunate consequence o ...
... Now that it is clear how important DNA is to generating and maintaining life, we will discuss the application of DNA to forensic testing. As discussed, DNA is composed of a string of nucleotides. Within each human cell, this string is approximately three billion bases long. A fortunate consequence o ...
Lecture 11 Gene1cs BIOL 335
... Conjuga>on of Hfr strains is similar to F+ strains, via a pilus F-containing chromosome is nicked at the F locus, and a single strand is passed through the pilus to the recipient cell DNA is transferred un>l complete, or un>l the pilus falls apart. Strands are replicated in both cells ...
... Conjuga>on of Hfr strains is similar to F+ strains, via a pilus F-containing chromosome is nicked at the F locus, and a single strand is passed through the pilus to the recipient cell DNA is transferred un>l complete, or un>l the pilus falls apart. Strands are replicated in both cells ...
overexpression of mcm protein potentially causes cancer
... protein to break sites and form sharply defined point (foci). Similar to procedures of RPA-GFP assay, Rad52-GFP assay is designed to localize double-strand DNA breaks sites. The test strains will be grown in the appropriate media, and the percent of cells containing RPA-GFP or Rad52-GFP foci will be ...
... protein to break sites and form sharply defined point (foci). Similar to procedures of RPA-GFP assay, Rad52-GFP assay is designed to localize double-strand DNA breaks sites. The test strains will be grown in the appropriate media, and the percent of cells containing RPA-GFP or Rad52-GFP foci will be ...
Printable PDF - Science Prof Online
... • Images used on this resource, and on the SPO website are, wherever possible, credited and linked to their source. Any words underlined and appearing in blue are links that can be clicked on for more information. PowerPoints must be viewed in slide show mode to use the hyperlinks directly. • Seve ...
... • Images used on this resource, and on the SPO website are, wherever possible, credited and linked to their source. Any words underlined and appearing in blue are links that can be clicked on for more information. PowerPoints must be viewed in slide show mode to use the hyperlinks directly. • Seve ...
DNA Questions #4 Questions on the PCR Process:
... __greater___________ (greater, less) the value in DNA fingerprinting. (There are two different answers heresmaller, greater and larger, less.) Explain why: _____Like identifying people in a room, it is better to use a characteristic like, “wears pink colored glasses” (not as common or frequent) than ...
... __greater___________ (greater, less) the value in DNA fingerprinting. (There are two different answers heresmaller, greater and larger, less.) Explain why: _____Like identifying people in a room, it is better to use a characteristic like, “wears pink colored glasses” (not as common or frequent) than ...
Epigenase™ 5-mC Hydroxylase TET Activity/Inhibition
... 2. Ensure that all buffers are in clear solution. Shake or vortex if these buffers precipitate. Antibody Binding to the Assay Plate 1. Determine the number of strip wells required. Leave these strips in the plate frame (remaining unused strips can be placed back in the bag. Seal the bag tightly and ...
... 2. Ensure that all buffers are in clear solution. Shake or vortex if these buffers precipitate. Antibody Binding to the Assay Plate 1. Determine the number of strip wells required. Leave these strips in the plate frame (remaining unused strips can be placed back in the bag. Seal the bag tightly and ...
DNA - Ms Futch
... 25. How were RFLP’s used in the impeachment trial investigation of former President Bill Clinton? Monica Lewinsky had a dress with semen on it from President Clinton. The FBI lab compared the DNA from the dress to that of Bill Clinton. A seven probe RFLP match was obtained. Between the DNA from the ...
... 25. How were RFLP’s used in the impeachment trial investigation of former President Bill Clinton? Monica Lewinsky had a dress with semen on it from President Clinton. The FBI lab compared the DNA from the dress to that of Bill Clinton. A seven probe RFLP match was obtained. Between the DNA from the ...
Section 13-2
... The Good With the Bad The manipulation of DNA allows scientists to do some interesting things. Scientists have developed many transgenic organisms, which are organisms that contain genes from other organisms. Recently, scientists have removed a gene for green fluorescent protein from a jellyfish and ...
... The Good With the Bad The manipulation of DNA allows scientists to do some interesting things. Scientists have developed many transgenic organisms, which are organisms that contain genes from other organisms. Recently, scientists have removed a gene for green fluorescent protein from a jellyfish and ...
10/16
... restriction enzyme is used for both sides, the plasmid is likely to religate to itself. ...
... restriction enzyme is used for both sides, the plasmid is likely to religate to itself. ...
GeNeViSTA Coffin Siris Syndrome: A Disorder of SWI/SNF Pathway
... subunits involved in DNA and protein-protein interactions which causes alteration of nucleosome structure in an ATP-dependent manner. flSC ”flemodeling the Structure of Chromatin), a closely related complex, was also identi ed in yeast. This complex is composed of 17 subunits and shows similarities to ...
... subunits involved in DNA and protein-protein interactions which causes alteration of nucleosome structure in an ATP-dependent manner. flSC ”flemodeling the Structure of Chromatin), a closely related complex, was also identi ed in yeast. This complex is composed of 17 subunits and shows similarities to ...
Ch. 5: Presentation Slides
... genomes = genetic contents of a cell • Genomic size increases with evolutionary complexity (in general!) • Size of DNA is measured in kb = kilobase ...
... genomes = genetic contents of a cell • Genomic size increases with evolutionary complexity (in general!) • Size of DNA is measured in kb = kilobase ...
Preview pptx - Sweetpotato Knowledge Portal
... Tree interpretation Clustering method; unweighted-pair group method with arithmetic means (UPGMA) use a sequential clustering algorithm. A tree is built in a stepwise manner, by grouping allele phenotypes /sequences /or groups of sequences– usually referred to as operational taxonomic units (OT ...
... Tree interpretation Clustering method; unweighted-pair group method with arithmetic means (UPGMA) use a sequential clustering algorithm. A tree is built in a stepwise manner, by grouping allele phenotypes /sequences /or groups of sequences– usually referred to as operational taxonomic units (OT ...
2014
... 7. [2 points] Which statement about the carbamoyl phosphate synthetase of mammals, which is used for pyrmidine biosynthesis, is true? A) It is located in the mitochondria. B) It is located in the nucleus. C) It uses NH4+ as a nitrogen source D) It uses glutamine as a nitrogen source Circle the corre ...
... 7. [2 points] Which statement about the carbamoyl phosphate synthetase of mammals, which is used for pyrmidine biosynthesis, is true? A) It is located in the mitochondria. B) It is located in the nucleus. C) It uses NH4+ as a nitrogen source D) It uses glutamine as a nitrogen source Circle the corre ...
Chapter 17 – Molecular genetics
... segments called Okazaki fragments ranging from 1 to 2 thousand nucleotides in lenth ...
... segments called Okazaki fragments ranging from 1 to 2 thousand nucleotides in lenth ...
switchSENSE® compatibility sheet
... Used for the extraction of proteins from (eukaryotic) cells, for instance in the lysis buffer RIPA (50 mM Tris-HCl pH 7.5, 150 mM NaCl, 1% NP40, 0.5% sodium deoxycholate, 0.1% SDS), or to solubilize GABA receptors. Can enhance or suppress interactions between DNA and proteins, often used to reduce a ...
... Used for the extraction of proteins from (eukaryotic) cells, for instance in the lysis buffer RIPA (50 mM Tris-HCl pH 7.5, 150 mM NaCl, 1% NP40, 0.5% sodium deoxycholate, 0.1% SDS), or to solubilize GABA receptors. Can enhance or suppress interactions between DNA and proteins, often used to reduce a ...
Nucleosome
A nucleosome is a basic unit of DNA packaging in eukaryotes, consisting of a segment of DNA wound in sequence around eight histone protein cores. This structure is often compared to thread wrapped around a spool.Nucleosomes form the fundamental repeating units of eukaryotic chromatin, which is used to pack the large eukaryotic genomes into the nucleus while still ensuring appropriate access to it (in mammalian cells approximately 2 m of linear DNA have to be packed into a nucleus of roughly 10 µm diameter). Nucleosomes are folded through a series of successively higher order structures to eventually form a chromosome; this both compacts DNA and creates an added layer of regulatory control, which ensures correct gene expression. Nucleosomes are thought to carry epigenetically inherited information in the form of covalent modifications of their core histones.Nucleosomes were observed as particles in the electron microscope by Don and Ada Olins and their existence and structure (as histone octamers surrounded by approximately 200 base pairs of DNA) were proposed by Roger Kornberg. The role of the nucleosome as a general gene repressor was demonstrated by Lorch et al. in vitro and by Han and Grunstein in vivo.The nucleosome core particle consists of approximately 147 base pairs of DNA wrapped in 1.67 left-handed superhelical turns around a histone octamer consisting of 2 copies each of the core histones H2A, H2B, H3, and H4. Core particles are connected by stretches of ""linker DNA"", which can be up to about 80 bp long. Technically, a nucleosome is defined as the core particle plus one of these linker regions; however the word is often synonymous with the core particle. Genome-wide nucleosome positioning maps are now available for many model organisms including mouse liver and brain.Linker histones such as H1 and its isoforms are involved in chromatin compaction and sit at the base of the nucleosome near the DNA entry and exit binding to the linker region of the DNA. Non-condensed nucleosomes without the linker histone resemble ""beads on a string of DNA"" under an electron microscope.In contrast to most eukaryotic cells, mature sperm cells largely use protamines to package their genomic DNA, most likely to achieve an even higher packaging ratio. Histone equivalents and a simplified chromatin structure have also been found in Archea, suggesting that eukaryotes are not the only organisms that use nucleosomes.