Mutation of Rice BC12/GDD1, Which Encodes a Kinesin
... Showed the same results in “PTCH1” Cell seeding and cell count after 48h. Cell number increase 2 times in patental, control shRNA transfection cell. But, cell proliferation decrease in Gli1-shRNA compare to patental, control shRNA cell. ...
... Showed the same results in “PTCH1” Cell seeding and cell count after 48h. Cell number increase 2 times in patental, control shRNA transfection cell. But, cell proliferation decrease in Gli1-shRNA compare to patental, control shRNA cell. ...
DNA microarrays and beyond: completing the journey from tissue to
... microarray data sets are collected and catalogued, certain combinations of geneexpression profiles will emerge as ‘fingerprints’ that identify particular pathways or cellular processes. For example, a stereotyped set of changes in a particular group of genes may identify a specific signal-transducti ...
... microarray data sets are collected and catalogued, certain combinations of geneexpression profiles will emerge as ‘fingerprints’ that identify particular pathways or cellular processes. For example, a stereotyped set of changes in a particular group of genes may identify a specific signal-transducti ...
Candidate genes for meat production and meat quality – the
... for increased growth rate is associated with increased MRF gene expression, regulation of satellite cell proliferation and differentiation, while selection for increased lean percentage is associated with increased maintenance of muscle tissue. Discovering genes regulated by the MRF genes – ChIP tec ...
... for increased growth rate is associated with increased MRF gene expression, regulation of satellite cell proliferation and differentiation, while selection for increased lean percentage is associated with increased maintenance of muscle tissue. Discovering genes regulated by the MRF genes – ChIP tec ...
Gene silencing in mammalian cells and the spread of DNA
... detectable) low-level transcription in most cells and very high spontaneous reactivation frequencies. At this stage many of the silenced alleles exhibited methylation patterns virtually identical to those observed for expressing alleles. The second stage, which apparently required the first stage as ...
... detectable) low-level transcription in most cells and very high spontaneous reactivation frequencies. At this stage many of the silenced alleles exhibited methylation patterns virtually identical to those observed for expressing alleles. The second stage, which apparently required the first stage as ...
A Statistical Approach to Literature
... Ideas for the Statistical Model • Observation: typically, some genes in the list are related to a given word, but the other genes are not (Few gene clusters are perfect!) • Assumption: the count of a term in a document follows Poisson distribution • Idea: the count of a term in one gene is either f ...
... Ideas for the Statistical Model • Observation: typically, some genes in the list are related to a given word, but the other genes are not (Few gene clusters are perfect!) • Assumption: the count of a term in a document follows Poisson distribution • Idea: the count of a term in one gene is either f ...
Zoo/Bot 3333
... (left table, below), where nutrient A allows the growth of a– auxotrophs, nutrient B allows for the growth of b– autxotrophs, etc. A (+) indicates the presence of the nutrient or drug, a (-) indicates its absence. The table below on the right shows the number of Timings of Samples Number of Colonies ...
... (left table, below), where nutrient A allows the growth of a– auxotrophs, nutrient B allows for the growth of b– autxotrophs, etc. A (+) indicates the presence of the nutrient or drug, a (-) indicates its absence. The table below on the right shows the number of Timings of Samples Number of Colonies ...
19EBarrays
... An example of how the model is imagined to generate the data for the jth gene. • Suppose p=0.05, α=12, α0=0.9, and v=36. • Generate a Bernoulli random variable with success probability 0.05. If the result is a success the gene is DE, otherwise the gene is EE. • If EE, generate λj from Gamma(α0=0.9, ...
... An example of how the model is imagined to generate the data for the jth gene. • Suppose p=0.05, α=12, α0=0.9, and v=36. • Generate a Bernoulli random variable with success probability 0.05. If the result is a success the gene is DE, otherwise the gene is EE. • If EE, generate λj from Gamma(α0=0.9, ...
Genome Editing of a CArG Element in the Mouse Genome
... blastocysts and implanted into surrogate mothers to yield chimeric mice. Fifth, the chimeric mice were bred to obtain mice that had inherited the mutant allele through the germline. Sixth, as part of the breeding, male mice expressing Cre recombinase in the germline were used to remove the antibioti ...
... blastocysts and implanted into surrogate mothers to yield chimeric mice. Fifth, the chimeric mice were bred to obtain mice that had inherited the mutant allele through the germline. Sixth, as part of the breeding, male mice expressing Cre recombinase in the germline were used to remove the antibioti ...
mRNA over-expression/rescue
... 3. Mutant (today) or morpholino (tomorrow) rescue 4. Epistasis: what genes can and cannot rescue your mutant? Where does your mutant gene lie in a pathway? Why inject DNA? • Gain-of-function experiments: over & ectopic expression • Dominant negative expts • Promoter analysis • Transgenics--in vivo l ...
... 3. Mutant (today) or morpholino (tomorrow) rescue 4. Epistasis: what genes can and cannot rescue your mutant? Where does your mutant gene lie in a pathway? Why inject DNA? • Gain-of-function experiments: over & ectopic expression • Dominant negative expts • Promoter analysis • Transgenics--in vivo l ...
The effect of isozymes on metabolic activity analysis
... The activity score represents the expected number of solutions in which a gene is active. For an active reaction associated with several isozymes, we think of each isozyme as having equal probability of being active and catalyzing the reaction. That is, we assume that isozymes are not concurrently a ...
... The activity score represents the expected number of solutions in which a gene is active. For an active reaction associated with several isozymes, we think of each isozyme as having equal probability of being active and catalyzing the reaction. That is, we assume that isozymes are not concurrently a ...
Powerpoint - Wishart Research Group
... • Most gene finders don’t handle overlapping or nested genes • Most can’t find non-protein genes (tRNAs) ...
... • Most gene finders don’t handle overlapping or nested genes • Most can’t find non-protein genes (tRNAs) ...
SPoRE - LCQB
... How to format the TF.txt matrix (only necessary for DSB model 7): This matrix contains the transcription factor binding sites for each gene. chr: chromosome number from 1 to N (optional - unused by SPoRE) position: position on the chromosome where the transcription factor binds to regulate the t ...
... How to format the TF.txt matrix (only necessary for DSB model 7): This matrix contains the transcription factor binding sites for each gene. chr: chromosome number from 1 to N (optional - unused by SPoRE) position: position on the chromosome where the transcription factor binds to regulate the t ...
March 20 - Mouse Genome Informatics
... • R package for differential gene expression that uses linear modeling for each gene in your data set • Expression data will be log-intensity values for Affy data • Designed to be used in conjunction with the affy package ...
... • R package for differential gene expression that uses linear modeling for each gene in your data set • Expression data will be log-intensity values for Affy data • Designed to be used in conjunction with the affy package ...
Microarrays - Harvard University
... determining its quality are: •How close is it to saturation? •How far above background is its signal? •How consistent is the measured ratio for each pixel in the spot? •How large is the spot? •In addition to a metric of spot quality, there may also be usful metrics of array quality, eg: •Is there ev ...
... determining its quality are: •How close is it to saturation? •How far above background is its signal? •How consistent is the measured ratio for each pixel in the spot? •How large is the spot? •In addition to a metric of spot quality, there may also be usful metrics of array quality, eg: •Is there ev ...
Supplementary Methods
... estimate the correct t value by running the test iteratively on permutations of experiments, thereby estimating the False Discovery Rate (FDR; 4). Indeed, our FDR analysis showed that the Dunnett’s test with m different from zero is more powerful, and much more reliable than classical T-test. To pri ...
... estimate the correct t value by running the test iteratively on permutations of experiments, thereby estimating the False Discovery Rate (FDR; 4). Indeed, our FDR analysis showed that the Dunnett’s test with m different from zero is more powerful, and much more reliable than classical T-test. To pri ...
Supplemental Figure and Methods
... anatomy (7), and mammalian phenotype ontology (8)) were ranked based on vectorspace similarity to the input text (9). For each ontology term, the term, including its description, and the input text were represented as two separate word vectors excluding common stop words, and the similarity of the w ...
... anatomy (7), and mammalian phenotype ontology (8)) were ranked based on vectorspace similarity to the input text (9). For each ontology term, the term, including its description, and the input text were represented as two separate word vectors excluding common stop words, and the similarity of the w ...
Presentation
... Why do bacterial and eukaryotic cells have different mechanisms of gene regulation? ...
... Why do bacterial and eukaryotic cells have different mechanisms of gene regulation? ...
Photosynthesis
... These enzymes are encoded by three structural genes which are adjacent to one another on the chromosome. They are controlled by one regulator gene that codes for a one repressor. ...
... These enzymes are encoded by three structural genes which are adjacent to one another on the chromosome. They are controlled by one regulator gene that codes for a one repressor. ...
Epigenetics Annual Research Report 2016
... liver cells when they divide need to produce more liver cells, otherwise cancer or other diseases may be the result. One of the epigenetic modifications we study is DNA methylation – a major biological DNA modification. Maintenance of this memory of cell identity involves a protein called UHRF1 whic ...
... liver cells when they divide need to produce more liver cells, otherwise cancer or other diseases may be the result. One of the epigenetic modifications we study is DNA methylation – a major biological DNA modification. Maintenance of this memory of cell identity involves a protein called UHRF1 whic ...
Characterizing the Imprintome
... expression so that one parent’s allele is selectively expressed. Together, these imprinted genes make up the imprintome. Scientists used to search for imprinted genes one by one, but thanks to modern sequencing techniques, they can now scan entire genomes. The precise size of the imprintome is uncer ...
... expression so that one parent’s allele is selectively expressed. Together, these imprinted genes make up the imprintome. Scientists used to search for imprinted genes one by one, but thanks to modern sequencing techniques, they can now scan entire genomes. The precise size of the imprintome is uncer ...
Document
... or sequences are disrupted, including the ori site, the promoter, and at least one of the genes encoding antibiotic resistance. Cut the plasmid near the promoter so that the inserted gene can be expressed. Cut the human DNA as close as possible to both ends of the gene of interest so that it can be ...
... or sequences are disrupted, including the ori site, the promoter, and at least one of the genes encoding antibiotic resistance. Cut the plasmid near the promoter so that the inserted gene can be expressed. Cut the human DNA as close as possible to both ends of the gene of interest so that it can be ...
HIT*nDRIVE: Multi-driver Gene Prioritization Based on Hitting Time
... Tumor cells accumulate genomic aberrations • Most are “passenger” aberrations while, • few are “driver” ones ...
... Tumor cells accumulate genomic aberrations • Most are “passenger” aberrations while, • few are “driver” ones ...
Chapter 15
... Gene Regulation I. Gene regulation in Prokaryotes A. Many related genes in prokaryotes are arranged sequentially along the chromosome. When one is transcribed, all are transcribed into one long (polycistronic) mRNA. B. Transcription begins when RNA polymerase first binds to a set of DNA bases that s ...
... Gene Regulation I. Gene regulation in Prokaryotes A. Many related genes in prokaryotes are arranged sequentially along the chromosome. When one is transcribed, all are transcribed into one long (polycistronic) mRNA. B. Transcription begins when RNA polymerase first binds to a set of DNA bases that s ...
Gene Section SNAI2 (SNAIL homolog 2) Atlas of Genetics and Cytogenetics
... It is involved in chick limb development and has conserved and divergent roles in the chick and mouse embryo. Human SNAI2 maps to the long arm of chromosome 8 (8q11.21), contains 3 exons and codes for a protein of 268bp (29KDa) with 5 zinc finger regions. This gene has been identified as downstream ...
... It is involved in chick limb development and has conserved and divergent roles in the chick and mouse embryo. Human SNAI2 maps to the long arm of chromosome 8 (8q11.21), contains 3 exons and codes for a protein of 268bp (29KDa) with 5 zinc finger regions. This gene has been identified as downstream ...
IJBT 10(2) 178-182
... individual motility as well as conception rates (CR %) was not found significant. Performance records of those two bulls revealed varying reproductive profile. Mu-5083 yielded freezable ejaculates with normal reproductive performance. While Mu-5525 buffalo bull used to show poor libido and had some ...
... individual motility as well as conception rates (CR %) was not found significant. Performance records of those two bulls revealed varying reproductive profile. Mu-5083 yielded freezable ejaculates with normal reproductive performance. While Mu-5525 buffalo bull used to show poor libido and had some ...