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doc BIOL 200 final notes
doc BIOL 200 final notes

... - during cell replication, DNA is assembled into nucleosomes shortly after replication fork passes: specific chaperones that bind to histones & assemble them together with newly replicated DNA into nucleosomes Structure of 30-nm fiber - most chromatin appear as fibers ~ 30 nm in diameter Interaction ...
Here - EdSpace
Here - EdSpace

... The CRISPR/Cas9 system stands as one of the new developments in genetic engineering used to modify any genomic sequence with high levels of specificity. The system first found in bacteria allows these species to develop resistance to foreign genetic elements, providing an acquired immunity.1 More re ...
Chapter 11 Transcription and RNA Processing
Chapter 11 Transcription and RNA Processing

... A Triplex of Sorts ...
Prenatal Exposure of Mice to the Human Liver Carcinogen Aflatoxin
Prenatal Exposure of Mice to the Human Liver Carcinogen Aflatoxin

... of mutations in the gpt transgenic mouse ...
The DNA repair helicase UvrD is essential for replication
The DNA repair helicase UvrD is essential for replication

... • RecG can regress forks quickly and extensively, but not processively • RecG and RecA likely act independently of each other • RecG doesn't like free Mg • …because free cations freeze Holliday junction geometry? • RecG can work on fully duplex 3-stranded structures, but RecA cannot • (since RecA re ...
Question 1 _____/ 30 points Question 2 _____/ 20 points Question 3
Question 1 _____/ 30 points Question 2 _____/ 20 points Question 3

... likely to be? Briefly explain your logic. (If you don’t have a calculator, set up the equation you would use to calculate this.) There should be only one recognition site for the MAT-cleaving nuclease in the entire genome since this cleavage event needs to be tightly regulated and only occurs at the ...
the genetic material
the genetic material

... Thymine, tell me the two complementary pairs. ...
- Ms. Ottolini`s Biology Wiki!
- Ms. Ottolini`s Biology Wiki!

...  Chaperonins = barrel shaped protein molecules that help wrap polypeptides around each other to create the final 3D shape of the full protein, which determines its function  Some have molecular groups added (sugars, lipids, phosphates, etc)… a specific example is a glycoprotein, a protein with a s ...
Document
Document

... Males are hemizygous for X chromosome gene expression, and a mutation of MeCP2 will lead to a loss or partial inactivation of the MeCP2 function. The severe phenotype in males results in early lethality. Theory 2 for female bias: Theory 1 does not explain why there are some males with Rett syndrome, ...
Section J Analysis and Uses of Cloned DNA
Section J Analysis and Uses of Cloned DNA

... • Length of target sequences:  Short target sequences amplify more easily, so often this distance is less than 500 bp, but, with optimization, PCR can amplify fragments over 10 kb in length. • Primer design: – The region to be amplified should be inspected for two sequences of  about 20 nt with a ...
View/print full test page
View/print full test page

... Syndrome Gene Panel OR the more comprehensive Autism/Intellectual Disability/Multiple Anomalies Gene Panel which includes the Rett/Atypical Rett/Angelman Syndrome Panel plus additional genes of interest. o Approximately 10-11% of individuals with AS will not have an identifiable AS-causing genetic a ...
Portfolio 2 - Biology2Nash
Portfolio 2 - Biology2Nash

... Nucleic Acid and Nucleotides DNA is made of long chains of nucleotides. Each nucleotide contains three basic parts: a base, a deoxyribose molecule, and a phosphate group. There are four different bases: adenine, cytosine, guanine, and thymine. Only one base is found in each nucleotide. Follow the di ...
brief talk
brief talk

... Test for loop condition – Fluorescent markers Can be detected by the robotic assistant Can have more than one type, allowing nested looping ...
Genetics and Heredity Completed notes
Genetics and Heredity Completed notes

... Each chromosome contains DNA. A gene is a part of DNA that contains the instructions that control a trait. You have different genes for each of the different traits that you inherit. Genes Each cell contains 46 chromosomes except for sex cells (eggs and sperm) which contain 23 chromosomes. Therefore ...
Personal Genetics: PCR Determination of PTC Tasters
Personal Genetics: PCR Determination of PTC Tasters

... 3’ …GGACACAACGGAAGTAG… 5’ ...
Genetics review
Genetics review

... responsible for the formation of various tissues and organs. Recent research suggests that it may be possible to replicate stem cells from sections of skin taken from adult mice, rather than having to use stem cells from the embryos of mice. In the future, human stem cells may be used to replace hum ...
Taster Lab Student Doc PDF
Taster Lab Student Doc PDF

... 3’ …GGACACAACGGAAGTAG… 5’ ...
Gene Silencing In Transgenic plants
Gene Silencing In Transgenic plants

... • It does not affect the transcription of gene locus but only cause sequence specific degradation of target mRNa • In both PTGS AND TGS genes are triggered by presence of dsRNA which are further cleaved into small RNA to become functional in no of gs process • stRNA and miRNA are originally intended ...
How exercise may regulate transcription
How exercise may regulate transcription

... Cellular and Molecular Exercise Physiology. See: Booth FW: Perspectives on molecular and cellular exercise physiology. J. Appl. Physiol, 65: 14611471, 1988. Molecular exercise physiology is a shortened version of the term used by Booth. A narrow definition of the term “molecular exercise physiology” ...
Monster Central Dogma - Lincoln Park High School
Monster Central Dogma - Lincoln Park High School

... 5. Suggest a substitution mutation in the DNA that would cause the first amino acid in the “# of Eyes” gene to change from alanine (Ala) to valine (Val). Write the original DNA codon, then the mutated DNA codon. (1) 6. There is a substitution mutation in the gene for Fangs in which the first DNA bas ...
Gel electrophoresis of restriction digest
Gel electrophoresis of restriction digest

... optimal separation; and (3) the gel is stained or, if ethidium bromide has been incorporated into the gel and electrophoresis buffer, visualized directly upon illumination with UV light. Gel electrophoresis can be used for a wide range of applications. It can be either analytical or preparative and ...
Coffee, B, Zhang, F, Warren, ST and Reines, D: Acetylated histones are associated with the FMR1 gene in normal but not fragile X syndrome cells. Nature Genetics 22:98-101 (1999).
Coffee, B, Zhang, F, Warren, ST and Reines, D: Acetylated histones are associated with the FMR1 gene in normal but not fragile X syndrome cells. Nature Genetics 22:98-101 (1999).

... Cell culture and drug treatments. EBV-transformed lymphoblastoid cell lines were derived from normal males or males with the typical clinical phenotype of fragile X syndrome. In normal cells, the FMR1 repeat is of normal length and methylation status, whereas those cells derived from patients exhibi ...
Preferential X-chromosome inactivation, DNA
Preferential X-chromosome inactivation, DNA

... ing. Silva and White (1988) demonstrated that in the human a number of loci, distinguished by differences in size of tandem repeat sequences (VNTR), may be differently methylated. The methylation pattern specific for a particular allele is heritable through several generations. The allele appears to ...
Evolution of Livestock Improvement
Evolution of Livestock Improvement

... The means by which the injected DNA is incorporated into the host genome is not completely understood. It has been proposed that repair enzymes in the developing embryo are activated by the injection of the unassociated DNA construct and create breaks in the host DNA which acts as an integration sit ...
Document
Document

... – The remaining 98.5% of the DNA contains – Control regions such as promoters and enhancers – Unique noncoding DNA – Repetitive DNA – Found in centromeres and telomeres – Found dispersed throughout the genome, related to transposable elements that can move or be copied from one location to another C ...
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Epigenomics

Epigenomics is the study of the complete set of epigenetic modifications on the genetic material of a cell, known as the epigenome. The field is analogous to genomics and proteomics, which are the study of the genome and proteome of a cell (Russell 2010 p. 217 & 230). Epigenetic modifications are reversible modifications on a cell’s DNA or histones that affect gene expression without altering the DNA sequence (Russell 2010 p. 475). Two of the most characterized epigenetic modifications are DNA methylation and histone modification. Epigenetic modifications play an important role in gene expression and regulation, and are involved in numerous cellular processes such as in differentiation/development and tumorigenesis (Russell 2010 p. 597). The study of epigenetics on a global level has been made possible only recently through the adaptation of genomic high-throughput assays (Laird 2010) and.
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