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Nucleic Acids notes
Nucleic Acids notes

... of a new DNA strand. End up with 2 new identical DNA molecules with one parent strand per new molecule and one new strand per new molecule ...
Cells
Cells

... A mutation occurs when the sequence of bases in a gene is altered. Mutations may interfere with an organisms ability to produce vital protein and may lead to a new variety within the species, hence, evolution. ...
Effects of mutations
Effects of mutations

... •RNA is similar to DNA in terms of its general properties, but its structure is different in several ways - single-stranded molecule that exists in helical form; can assume secondary and tertiary levels of complexity, leading to specialized forms of RNA (tRNA and rRNA) ...
The BCM Microarray Core Facility
The BCM Microarray Core Facility

... genomic research. We house instrumentation supporting Affymetrix, Agilent, NimbleGen, Luminex, and Illumina platforms. The MCF provides expertise in the following applications: gene expression profiling using arrays, array comparative genomic hybridization (aCGH), SNP genotyping, and nextgeneration ...
DNA intro website questions
DNA intro website questions

... 2. How many amino acids regularly occur in proteins? (Chromosome Structure) 3. What is a nucleosome? (DNA structure) 4. What does DNA stand for? 5. What are the two purines? 6. What are the two pyrimidines? 7. The human genome contains over_____________ base pairs. (DNA Replication) 8. What splits t ...
AIMS Review Packet
AIMS Review Packet

... both expressed in a heterozygote. Horses that have a both a red and white coat are said to have a roan coat (both red and white hairs). Horses that have neither of the dominant alleles have a blonde coat. Cross two individuals with roan coats. Calculate the phenotypic and genotypic ratios. ...
Honors Biology Module 7 Cellular Reproduction
Honors Biology Module 7 Cellular Reproduction

... Messenger RNA reads this sequence and makes a “negative image” of the relevant portion of DNA. It then takes this series of nucleotide base sequences out to the ribosome. Once at the ribosome, each codon (set of three nucleotide bases) on the mRNA will attract a particular anticodon (set to three nu ...
Structure and function of DNA
Structure and function of DNA

... It is the key that read the code on the mRNA. Each amino acid has its own tRNA, which binds to it and carries it to the growing end of a polypeptide chain. ...
Structure and function of DNA
Structure and function of DNA

... It is the key that read the code on the mRNA. Each amino acid has its own tRNA, which binds to it and carries it to the growing end of a polypeptide chain. ...
Nucleic acids Nucleic acids are information
Nucleic acids Nucleic acids are information

... – Cancer cells, which divide uncontrollably result from mutations in genes whose protein products affect the cell cycle. Proto-oncogene (a normal gene that promotes cell division) ...
DNA polymerase - yusronsugiarto
DNA polymerase - yusronsugiarto

... • Several methods. One is random primers labeling: • use 32P-labeled dNTPs • short random oligonucleotides as primers (made synthetically) • single stranded DNA template (made by melting double stranded DNA by boiling it) • DNA polymerase copies the DNA template, making a new strand that incorporate ...
11_DNA is the genetic material (MRU)
11_DNA is the genetic material (MRU)

... 4. RNA AND PROTEIN While DNA is the genetic material for the vast majority of organisms, there are some viruses that use RNA as their genetic material. These viruses can be either single or double stranded. Examples include SARS, influenza, hepatitis C and polio, a ...
gen-305-16-hw-4-key
gen-305-16-hw-4-key

... Q3. Some vectors used in cloning experiments contain bacterial promoters that are adjacent to unique cloning sites. This makes it possible to insert a gene sequence next to the bacterial promoter and express the gene in bacterial cells. These are called expression vectors. If you wanted to express a ...
Mutations
Mutations

... – Change in second position often either does nothing or changes one amino acid for a similar one ...
Chapter 8 Lecture Notes
Chapter 8 Lecture Notes

... c. RNA polymerase can be blocked by repressor protein when repressor protein binds to the operator. This prevents transcription. Fig. 12 (step 2). i. This occurs when lactose is absent. ii. Repressor protein is reversibly bound to the operator. d. When lactose is present, lactose is transported into ...
BL414 Genetics Spring 2006  page Test 2
BL414 Genetics Spring 2006 page Test 2

... and those of higher eukaryotes, including the following terms in your description: regional centromeres, alpha satellite, point centromeres and localized centromeres. Yeast and higher eukaryotes both have localized centromeres which have a single specific site on the chromosome. Yeast centromeres ar ...
Promoters - Pennsylvania State University
Promoters - Pennsylvania State University

... Enhancers : Additional DNA sequences that regulate transcription • Enhancers cause an increase in expression of a gene. • Can act in either orientation. • Can act in a variety of positions: – 5’ to gene (similar to an upstream activation sequence) – Internal to a gene (e.g. in an intron) – 3’ to a ...
KTH | BB2430 Gene Technology and Molecular Biology, theory 5.5
KTH | BB2430 Gene Technology and Molecular Biology, theory 5.5

... strategies that hold promise to solve real-world problems; some are related to the diagnosis and treatment of disease, others to the use of genetically modified organisms for detoxification of the environment or production of biofuels, while still others deal with the engineering of proteins to adap ...
Human Genetics Lec 4
Human Genetics Lec 4

... cytoplasm, most ribosomes become attached to the endoplasmic reticulum and begin the task of protein synthesis. Proteins are made from a standard set of amino acids, which are joined end to end to form the long polypeptide chains of protein molecules. Each polypeptide chain may have as many as 100 t ...
Slide 1
Slide 1

...  RAPD markers need to be converted to stable PCR markers  The polymorphic RAPD marker band is isolated from the gel  It is used a template and re-PCRed  The new PCR product is cloned and sequenced  Once the sequence is determined, new longer and specific primers can be designed ...
Group 4 members
Group 4 members

... – High throughput deep sequencing  analyze pools of cells, get genome-wide overviews of genes and enable rapid assessment of the spectrum of genes, assigning genes to phenotypes with high saturation and accuracy; ...
Trans-HHS Workshop: Diet, DNA Methylation
Trans-HHS Workshop: Diet, DNA Methylation

... evaluation of genomic DNA methylation status is important for the study of cell growth regulation, tissue-specific differentiation (2,4,7) and carcinogenesis (6). Most recently, an interaction was described between folate status and a common mutation in a key enzyme of the onecarbon metabolism that ...
Soybean (Glycine max L.) Nuclear DNA Contains
Soybean (Glycine max L.) Nuclear DNA Contains

... transcribed tufA genes exist. Equivalent fragments were cut from both cDN A samples (cDNAl and cDNA2) and used as probes in Southern experiments (Fig. 1). We see that either probe interacts with four HindIII fragments albeit in adifferential manner: cDNA2 (panel I) gives a strong signal with the two ...
Transposable Elements
Transposable Elements

... element excises during development. ...
Chapter 19. - Kenston Local Schools
Chapter 19. - Kenston Local Schools

... regulatory proteins attach to 5’ end of mRNA prevent attachment of ribosomal subunits & ...
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Epigenomics

Epigenomics is the study of the complete set of epigenetic modifications on the genetic material of a cell, known as the epigenome. The field is analogous to genomics and proteomics, which are the study of the genome and proteome of a cell (Russell 2010 p. 217 & 230). Epigenetic modifications are reversible modifications on a cell’s DNA or histones that affect gene expression without altering the DNA sequence (Russell 2010 p. 475). Two of the most characterized epigenetic modifications are DNA methylation and histone modification. Epigenetic modifications play an important role in gene expression and regulation, and are involved in numerous cellular processes such as in differentiation/development and tumorigenesis (Russell 2010 p. 597). The study of epigenetics on a global level has been made possible only recently through the adaptation of genomic high-throughput assays (Laird 2010) and.
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