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No Slide Title
No Slide Title

... Addition of a 5’ cap Addition of a 3’ tail ...
Chromosomes come in pairs
Chromosomes come in pairs

... sequence, or any other alteration of the genome that is not manifested as reciprocal recombination. ...
DNA SEQUENCING (using an ABI automated sequencer)
DNA SEQUENCING (using an ABI automated sequencer)

... DNA polymerase) in conjunction with a mix of deoxynucleotide triphosphates (dNTPs) and dideoxynucleotide triphosphates (ddNTPs). Since dideoxynucleotides terminate the growth of the DNA polymer once they are incorporated (since the hydroxyl at the 3' position is absent), a series of fragments is pro ...
20.1 Structural Genomics Determines the DNA Sequences of Entire
20.1 Structural Genomics Determines the DNA Sequences of Entire

... c. a set of fragments generated by a restriction enzyme d. a small DNA fragment used in sequencing ...
Gene Expression
Gene Expression

... RNA polymerase transcribes both the exons and introns, producing a long RNA molecule. Enzymes in the nucleus then add further nucleotides at the beginning (cap) and end (tail) of the RNA transcript. Other enzymes cut out the RNA introns and splice together the exons to form the true mRNA, which move ...
Transgenic Animal Models
Transgenic Animal Models

... Mate this line with the desired Cre recombinase mouse strain. E.g., one that has the Cre recombinase in the hippocampus. There is a very large number of Cre recombinase mouse lines, each with tissue-specific, developmentally specific, or exogenous chemical specific properties (e.g., responds to tetr ...
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... • Antibiotics help cure infections by decreasing the bacterial population to a level that the human immune system can handle • Gonorrhea is treated with antibiotics ...
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Chapter 2

... •Mutations in these regions have mild and severe effects on transcription. ...
Evolution of genomes
Evolution of genomes

... Over the course of evolution, mane large-scale genome rearrangements are known to have occurred. This involve such processes as large-scale inversions and transpositions (often involving the movement of genetic material from one chromosome to another) as well as linking or breaking up chromosomes. ...
Mutation - NIU Department of Biological Sciences
Mutation - NIU Department of Biological Sciences

... Eukaryotes often contain very short (200-500 bp) elements that contain the ends of a longer DNA transposon and miscellaneous junk inside. They move to new locations using the transposase enzyme from a full length element. Most bacterial TEs are DNA only. In eukaryotes, DNA transposable elements occu ...
AP Bio Ch.18 “Genetics of Viruses and Bacteria” The Genetics of Viruses
AP Bio Ch.18 “Genetics of Viruses and Bacteria” The Genetics of Viruses

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U4Word
U4Word

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DNA and RNA Chapter 12

... 1. On a sheet of paper, draw a curving or zig-zagging line that divides the paper into two halves. Vary the bends in the line as you draw it. Without tracing, copy the line on a second sheet of paper. 2. Hold the papers side by side, and compare the lines. Do they look the ...
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File

... Steps in processing 1. Add the cap – cells have different types of caps (5 cap is made up of modified Guanine) 2. 3 tail 3. Splicing is the removal of the introns Eukaryotic organisms – interrupted gene has two parts 1. Exons – DNA sequence which is transcribed into RNA to be transferred into protei ...
Biology 120 Lab Exam 2 Review Session
Biology 120 Lab Exam 2 Review Session

... 1. Ferns have a complex life cycle called an __alternation______ __of__ ______generations______. 2. In the fern, ___sporocytes___ undergo meiosis to form haploid cells called spores. 3. Crossing over occurs in ___Prophase I___ of meiosis. 4. In the fern life cycle, the multicellular _____gametophyt ...
Chapter 13, 14 Rev
Chapter 13, 14 Rev

... The sequence of nitrogenous bases on one strand of DNA may determine the sequence of: a. Fatty acids in a fat molecule b. Amino acids in a protein molecule c. Sugars in a polysaccharide molecule d. All of the above choices are correct e. Bases in a protein molecule The sequence of nitrogen bases on ...
Bio 101 Homework 2 Prof. Fournier
Bio 101 Homework 2 Prof. Fournier

... selective breeding for particular traits recombination of genes during mitosis mutations present only in body cells natural selection of favorable traits ...
Slide 1
Slide 1

... Development of Plasmid Vectors Plasmids serve as important tools in genetics and biochemistry labs, where they are commonly used to multiply or express particular genes. Plasmids used in genetic engineering are called vectors. Vectors are vehicles to transfer genes from one organism to another and ...
e) Describe the structure of a bacterial chromosome including the
e) Describe the structure of a bacterial chromosome including the

... 3. cell wall – peptidoglycan = sugars cross linked by short polypeptide chains 4. bi-lipid cell membrane 5. Capsule – covers cell = A layer of polysaccharide. Often present in pathogenic bacteria that protects the bacteria cell from environmental - antibiotics or phagocytosis by white blood cells, h ...
ch. 12 Biotechnology-notes-ppt
ch. 12 Biotechnology-notes-ppt

... • New genetic varieties of animals and plants are being produced – A plant with a new trait can be created using the Ti plasmid ...
dominant gene
dominant gene

... adding specific characteristics that give the plant new benefits. For example, biotech rice contains more iron than regular rice. 2. A specific gene is taken from one plant and placed into the DNA of a second plant. The second plant will then grow with all its own traits plus the one from the first ...
41. Situations in which one allele for a gene is not
41. Situations in which one allele for a gene is not

... determine whether a particular allele of a gene is dominant or recessive. c. identify similarities and differences in the genomes of different kinds of organisms. d. compare the phenotypes of different organisms. A Punnett square shows all of the following EXCEPT a. the genotypes of the offspring. b ...
Anatomy and Physiology BIO 137
Anatomy and Physiology BIO 137

... • Given that PCR involves very high temperatures, it is imperative that a heat-stable DNA polymerase be used in the reaction. • Most DNA polymerases would denature (and thus not function properly) at the high temperatures of PCR. ...
HOMEWORK #8 KEY 1. Draw a restriction map of the 20 kb DNA
HOMEWORK #8 KEY 1. Draw a restriction map of the 20 kb DNA

... representing the virus genome which shows the relative positions (ie, order) of the restriction sites. Either include a scale (in kb) or indicate the approximately distances between restriction sites. ...
Gene Section PEG3 (paternally expressed 3)  Atlas of Genetics and Cytogenetics
Gene Section PEG3 (paternally expressed 3) Atlas of Genetics and Cytogenetics

... There are several studies revealed murine Peg3 acts as an intermediary between p53 and Bax in a cell death pathway activated by DNA damage in primary mouse cortical neurons, inhibiting Peg3 activity blocks p53induced apoptosis (Johnson et al., 2002). Pw1/Peg3 interacts with a p53-inducible gene prod ...
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Epigenomics

Epigenomics is the study of the complete set of epigenetic modifications on the genetic material of a cell, known as the epigenome. The field is analogous to genomics and proteomics, which are the study of the genome and proteome of a cell (Russell 2010 p. 217 & 230). Epigenetic modifications are reversible modifications on a cell’s DNA or histones that affect gene expression without altering the DNA sequence (Russell 2010 p. 475). Two of the most characterized epigenetic modifications are DNA methylation and histone modification. Epigenetic modifications play an important role in gene expression and regulation, and are involved in numerous cellular processes such as in differentiation/development and tumorigenesis (Russell 2010 p. 597). The study of epigenetics on a global level has been made possible only recently through the adaptation of genomic high-throughput assays (Laird 2010) and.
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