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Day1-UVM-2ndvisit-Pombe
Day1-UVM-2ndvisit-Pombe

... than chlorine, chlorine dioxide, and potassium permanganate. And through catalysis, H2O2 can be converted into hydroxyl radicals (.OH) with reactivity second only to fluorine. • Grow the yeast and treat the control group with buffer (HBSS) and the treated group with buffer containing 0.5 mM H2O2 • I ...
3. What are macromolecules?
3. What are macromolecules?

... The four main classes of organic compounds (carbohydrates, lipids, proteins, and nucleic acids) that are essential to the proper functioning of all living things are known as polymers or macromolecules. All of these compounds are built primarily of carbon, hydrogen, and oxygen but in different ratio ...
Bench Guide
Bench Guide

... What is RNA? RNA is a biological macromolecule that serves a number of different functions. Messenger RNA (mRNA), transcribed from DNA, serves as a template for synthesis of proteins. Protein synthesis is carried out by ribosomes, which consist of ribosomal RNA (rRNA) and proteins. Amino acids for p ...
Introductory Molecular and Cell Biology (Shors, Kostman)
Introductory Molecular and Cell Biology (Shors, Kostman)

... unifying theme in this course is the oneness of all earthly life forms, attesting to their common evolutionary origin. As described in the COLS's learning objectives, in addition to studying the factual content of the science, we will also consider its historical development, experimental basis, and ...
DNA replication
DNA replication

... Cell will not enter the mitotic phase unless all the DNA has replicated. ...
PDF format
PDF format

... Each of us began as a single cell, so one important question is: How did that single cell develop into a body with more than a trillion cells? The production of such a large number of body cells is accomplished by many, many repeats of a cycle of cell division in which one cell divides to form two c ...
Gene Therapy
Gene Therapy

... Gene therapy can be broadly defined as the transfer of defined genetic material to specific target cells of a patient for the ultimate purpose of preventing or altering a particular disease state. Two main approaches to gene therapy include in vivo and ex vivo. Ex vivo gene transfer techniques usual ...
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... C8. Maternal effect genes exert their effects because the gene products are transferred from nurse cells to oocytes. The gene products, mRNA and proteins, do not last a very long time before they are eventually degraded. Therefore, they can only exert their effects during early stages of embryonic d ...
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... C8. Maternal effect genes exert their effects because the gene products are transferred from nurse cells to oocytes. The gene products, mRNA and proteins, do not last a very long time before they are eventually degraded. Therefore, they can only exert their effects during early stages of embryonic d ...
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... ability to take control of host cells, make new virus particles that can then infect other cells, and transmit to other hosts. However, the flu virus must change its topography to adjust to new host species. As such, the protein hemagglutinin allows viruses to enter host cells and uses a “globular h ...
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The HSV Manual - McGovern Institute for Brain Research at MIT
The HSV Manual - McGovern Institute for Brain Research at MIT

... the highest titers possible. This gradual increase in titer has benefited most of those using my vectors, who wanted greater spread and more robust expression in vivo. However, it had the unintended consequence, in a very few regions of the brain, of causing XFP toxicity immediately around the site ...
Fulltext: english, pdf
Fulltext: english, pdf

... sporadic tumours. It is not necessarily always the same gene, it is sufficient that mutation has occurred in an important gene and expression of other genes with malignant phenotype can be altered by epigenetic mechanisms, if not by gene mutation. What makes this key mutation a necessity is its irre ...
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... ability to take control of host cells, make new virus particles that can then infect other cells, and transmit to other hosts. However, the flu virus must change its topography to adjust to new host species. As such, the protein hemagglutinin allows viruses to enter host cells and uses a “globular h ...
BIO305 - National Open University of Nigeria
BIO305 - National Open University of Nigeria

... genetic manipulations similar to those that can be performed using bacteria. Yeast mutants have been important in understanding many fundamental processes in eukaryotes, including DNA replication, transcription, RNA processing, protein sorting and regulation of cell division. ...
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... 2. Understand how the following terms apply to RNA structure: phosphodiester bonds, 5' and 3 ends, nucleosides, complementary base pairing, stem loops. 3. Compare and contrast DNA and RNA structure. 4. What is a gene? What is gene expression? *Understand transcription, translation, and RNA processin ...
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Biology Common Syllabus

... Matter and energy transformations are involved in all life processes, such as photosynthesis, growth and repair, cellular respiration, and the need of living systems for continual input of energy. All single-celled and multicellular organisms have the same basic needs: water, air, a source of energy ...
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Gene and Genome Evolution

... pressure. In a comparison of the human and mouse genomes, 165 Mbp of DNA associated with non-functional transposon sequences were identified in both species. These had about 67% identical bases, which implied a rate of 0.46 substitutions per position over the 75 million years since the human and mou ...
Maple Syrup Urine Disease
Maple Syrup Urine Disease

... encoding dihydrolipoyl transacylase (E2) component of human branched chain alpha-keto acid dehydrogenase and characterization of an E2 pseudogene. J. Biol. Chem. 267: 24090-24096,1992. 4. Mitsubuchi, H.; Nobukuni, Y.; Akaboshi, I.; Indo, Y.; Endo, F.; Matsuda, I.: Maple syrup urine disease caused by ...
IRANIAN BIOLOGICAL RESOURCE CENTER
IRANIAN BIOLOGICAL RESOURCE CENTER

... decades will lead to the sustainable development of preparation of sufficient food and natural resources like water, soil and weather as well as biological resources which is one of the greatest challenges, has been faced by human community. Because of the great importance of biological resources in ...
Description
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... Nested PCR: increases the specificity of DNA amplification, by reducing background due to non-specific amplification of DNA. Two sets (instead of one pair) of primers are used in two successive PCRs. In the first reaction, one pair of primers is used to generate DNA products, which besides the inten ...
supplementary materials and methods
supplementary materials and methods

... TaqMan real-time quantitative RT-PCR analysis was used to measure expression levels of LMNB1 in one affected individual (III-4) and three healthy controls. mRNA was extracted from the lymphoblastoid cell lines and retrotranscribed using the “TaqMan gene expression cells-to-ct” kit under the conditio ...
Science Home Learning Task Year 7 Body systems
Science Home Learning Task Year 7 Body systems

... contain? Use the last page to show your findings. ...
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Vectors in gene therapy

Gene therapy utilizes the delivery of DNA into cells, which can be accomplished by several methods, summarized below. The two major classes of methods are those that use recombinant viruses (sometimes called biological nanoparticles or viral vectors) and those that use naked DNA or DNA complexes (non-viral methods).
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