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Construction of a new cloning vector utilizing a cryptic plasmid and
Construction of a new cloning vector utilizing a cryptic plasmid and

... Osaka University of Pharmaceutical Sciences, Takatsuki, Osaka 569-1041, Japan Received 25 August 1998; received in revised form 16 September 1998; accepted 18 September 1998 ...
miRNASelect™ pEGP-mmu-mir-21 Expression Vector
miRNASelect™ pEGP-mmu-mir-21 Expression Vector

... which recruit a large protein complex known as the RNA-induced silencing complex (RISC) to target mRNAs which are subsequently silenced. Diverse functions have been attributed to miRNAs including the regulation of cellular differentiation, proliferation, and apoptosis. Moreover, significant evidence ...
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... A. DNA – RNA – protein B. protein – RNA – DNA C. DNA – protein – RNA D. RNA – protein – DNA ...
6.6 Meiosis and Genetic Variation
6.6 Meiosis and Genetic Variation

... – Explain how crossing over during meiosis creates genetic diversity – Fruit fly gametes each have four chromosomes, representing 2^4, or 16, possible chromosome combinations. How many chromosome combinations could result from fertilization between a fruit fly egg and a sperm cell? – Suppose two gen ...
Screening for Recombinants
Screening for Recombinants

... rec A minus, but there are other recombinases present). You can transform the plasmid into an E. coli strain deficient in more recombinases than just the recA. Some strains like SURE® cells from Stratagene are deficient in recombinases rec B and rec J and may allow you to propagate the unstable inse ...
Founder mutations: evidence for evolution?
Founder mutations: evidence for evolution?

... Studying the prevalence of this haplotype in various populations has led to the conclusion that the founder lived in Central Europe between 60 and 70 generations ago and was probably of Celtic origin. This mutation has been carried through space and time in that European’s descendants to now include ...
Mitosis
Mitosis

... 29. What are all the chromosomes except the sex chromosomes called? _________________. 30. What chromosomes are needed to produce a female? _______ male? ________ 31. Give three examples of sex-linked disorders. __________________________________ __________________________________ __________________ ...
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... A mouse skin cell contains 40 chromosomes. Based on this information, how many chromosomes would a mouse SPERM cell contain? ...
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Hox

... • Regulatory genes: code for signal proteins and transcription factor proteins – SP: target particular groups of cells for gene expression ...
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... 29. What are all the chromosomes except the sex chromosomes called? _________________. 30. What chromosomes are needed to produce a female? _______ male? ________ 31. Give three examples of sex-linked disorders. __________________________________ __________________________________ __________________ ...
DNAandproteinsynthesis
DNAandproteinsynthesis

... The first initiation stage of translation brings together mRNA, a tRNA bearing the first amino acid of a polypeptide, and the two ribosomal subunits. The small ribosomal sub-unit attaches to a specific nucleotide sequence on the mRNA strand just ‘upstream’ the initiation codon (AUG) where translatio ...
69 Evidence from DNA
69 Evidence from DNA

... The genetic code is made up of four “letters” (A, T, C, and G), each of which stands for one of four related chemicals that are strung together in the DNA. The order of these letters provides information. Since the sequences of the genes do not vary much among people, fingerprinting genes would not ...
Prokaryotic Gene Regulation
Prokaryotic Gene Regulation

... • b-galactosidase • Gene Regulation • Biochemical Regulation ...
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Supplementary METHODS

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... Can DNA be improved on? Although it is prodigious at encoding genetic information, it does so with an alphabet of only four letters. If this alphabet were extended, more information could be stored. Ichiro Hirao and Shigeyuki Yokoyama at the RIKEN Genomic Sciences Center in Yokohama, Japan, and thei ...
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Bioinformatics - University of Hawaii
Bioinformatics - University of Hawaii

... expression, proteomics is, in effect, the “product” science made possible by bioinformatics A proteome is the collection of all proteins expressed in a cell at a given time Every organism has 1 genome, but many proteomes In addition to “high throughput” protein analysis, proteomics is researched thr ...
Review Session One
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... 1. List properties and examples for the four tissue types. Relate structure and function. 2. Describe the respiration processes in a mammal. Compare to a fish, insect, and amphibian. 3. How does oxygen and carbon dioxide travel in the blood? 4. How does a nephron work to concentrate urine yet excret ...
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... Transcription Activators of Eukaryotes • The general transcription factors by themselves dictate the starting point and direction of transcription but they are capable of sponsoring only a low level of transcription or basal transcription • Transcription of active genes in cells rises above the bas ...
Genetics - Stjosephcs.org
Genetics - Stjosephcs.org

... DNA is found in all living cells – It controls all functions inside a cell – It stores all the genetic information for an entire living organism – Single cell like an amoeba – Multi cell like a human ...
Meiosis Notes:
Meiosis Notes:

... Each organism must inherit a single copy of every gene from each of its _______________. (Humans = _______ chromosomes) _______________ (sex cells) are formed by a process that _____________ the two sets of genes so that each ______________ends up with just one set. (____ splits to _____) Chromosome ...
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Vectors in gene therapy

Gene therapy utilizes the delivery of DNA into cells, which can be accomplished by several methods, summarized below. The two major classes of methods are those that use recombinant viruses (sometimes called biological nanoparticles or viral vectors) and those that use naked DNA or DNA complexes (non-viral methods).
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