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animations of selected figures
animations of selected figures

... Fig. 13.1 Plasmid R1Copy Number Origin of Promoter 2 Copy number control region replication CopA RNA Prevents translation of tap ...
DNA cloning intro - Sundarban Hazi Desarat College
DNA cloning intro - Sundarban Hazi Desarat College

... BACs - Bacterial Artificial Chromosomes These chimeric DNA molecules use a naturallyoccurring low-copy number bacterial plasmid origin of replication, such as that of F-plasmid in E. coli. Can be cloned as a plasmid in a bacterial host, and its natural stability generally permits cloning of large pi ...
Lab Gel Electrophoresis
Lab Gel Electrophoresis

... cellular and subcellular structure, basic cell chemistry, transport across cell membranes, mitosis, meiosis, metabolism, photosynthesis, DNA structure and replication, protein synthesis, and patterns of inheritance. This course is required as a prerequisite for most other four-credit Biology courses ...
Incomplete dominance and Codominance Note
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... a) Codominant alleles are symbolized by a with a b) Codominant alleles express allele phenotypes. c) The presence of both phenotypes is commonly referred to as " ...
Human Monoclonal Antibodies
Human Monoclonal Antibodies

... that recognize tyrosine were randomly mutagenized to form a library of different tRNA synthetases that still recognize the same tRNA, but might attach different amino acids. Next, these library clones (MutTyrRS) were expressed in a cell containing another plasmid carrying genes for the amber tRNA (M ...
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... – Behavior of genes mimics movement of chromosomes – Allelic variants segregate equally (1st Law) – Different genes usually sort independently (Mendel’s 2nd Law) ...
Nucleotide excision repair II: from yeast to mammals
Nucleotide excision repair II: from yeast to mammals

... aQuestion marks indicate characteristics inferred on the basis of the predicted amino acid sequences of the proteins. protein known to be specifically involved in the preferential repair of active genes. The ERCC1 gene did not alleviate the NER defect in cell lines of any XP, CS or PIBIDS complement ...
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Year 1 Medical Genetics Final Examination March 1, 1996
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Practice Exam 3, Biology 211, Sections 1 and 4, Fall, 2007
Practice Exam 3, Biology 211, Sections 1 and 4, Fall, 2007

... c. The number of DNA molecules per chromatid varies between one and two depending on the time in the cell cycle. d. The number of DNA molecules per chromatid is described by the term ploidy. Thus, diploid cells contain two, tetraploid four, etc. e. Each gene is a DNA molecule, and chromosomes contai ...
Evo Lab 3 BLAST
Evo Lab 3 BLAST

... these species are available for anyone in the world to access via the Internet. Why is this information important? Being able to identify the precise location and sequence of human genes will allow us to better understand genetic diseases. In addition, learning about the sequence of genes in other s ...
Practice Exam 3, Biology 211, Sections 1 and 4, Fall, 2007
Practice Exam 3, Biology 211, Sections 1 and 4, Fall, 2007

... c. The number of DNA molecules per chromatid varies between one and two depending on the time in the cell cycle. d. The number of DNA molecules per chromatid is described by the term ploidy. Thus, diploid cells contain two, tetraploid four, etc. e. Each gene is a DNA molecule, and chromosomes contai ...
Product Manual Plant DNA Isolation Reagent
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... standard protocol. For samples which do not contain PCR inhibitors, using this purification method may not be helpful. ・ We recommend using Takara Ex Taq ® Hot Start Version (Product code: RR006A) for amplification. 3.The extracted DNA solution contains too much RNA. ・ RNA cannot always be exclude ...
Supplementary Information (doc 46K)
Supplementary Information (doc 46K)

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... Most human cells are between 10 and 15 micrometres (μm) in diameter (1 μm is onethousandth of a millimetre). Nerve cells may have extensions that are up to a metre long and muscle cells may be up to 30 cm long. However, both nerve and muscle cells are too thin to be seen with the naked eye. Human eg ...
CMSC 838T – Lecture 10 Genomics
CMSC 838T – Lecture 10 Genomics

... O ESTs from different individuals / strains of one species O Distinguishing between mutations and sequencing errors O Genomic & protein databases provide additional clues ...
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Transposable elements
Transposable elements

... Cointegration = movement of a transposon from one genome (e.g., plasmid) to another (e.g., chromosome) integrates transposon to both genomes (duplication). ...
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Vectors in gene therapy

Gene therapy utilizes the delivery of DNA into cells, which can be accomplished by several methods, summarized below. The two major classes of methods are those that use recombinant viruses (sometimes called biological nanoparticles or viral vectors) and those that use naked DNA or DNA complexes (non-viral methods).
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