REVIEW Why Do Bacterial Plasmids Carry Some Genes and Not
... 1987). One expected result is that plasmid geneswill be able to evolve more rapidly than ...
... 1987). One expected result is that plasmid geneswill be able to evolve more rapidly than ...
Comparative sequence analysis of the long repeat regions and
... conserved and there was no satisfactory alternative ...
... conserved and there was no satisfactory alternative ...
Genomic Organization of Evolutionarily Correlated Genes in
... these periods (inset), from 100 kb to 1 Mb, is scanned with a higher resolution after subtracting the basal line drift due to chromosomal proximity. The labels indicate peak periods in kilobase pairs. In particular, if periodicity was not present, scores would typically be on the same order as those ...
... these periods (inset), from 100 kb to 1 Mb, is scanned with a higher resolution after subtracting the basal line drift due to chromosomal proximity. The labels indicate peak periods in kilobase pairs. In particular, if periodicity was not present, scores would typically be on the same order as those ...
RT2 Profiler PCR Arrays: Pathway-focused gene
... pathway or disease state and three RNA quality control elements (see Figure 2 for the layout of a typical RT2 Profiler PCR Array). Researchers are able to focus on genes related to their biological pathway or disease state with our pre-designed pathway- or application-specific gene panels. By limiti ...
... pathway or disease state and three RNA quality control elements (see Figure 2 for the layout of a typical RT2 Profiler PCR Array). Researchers are able to focus on genes related to their biological pathway or disease state with our pre-designed pathway- or application-specific gene panels. By limiti ...
Cloning and sequencing of a gene encoding acidophilic amylase
... acidocaldarius ATCC 27009 chromosomal DNA was constructed as follows. Chromosomal DNA from B. acidocaldarius was isolated according to Marmur (1961). The DNA was digested partially with HaeIII. DNA fragments of 5.0 to 6.5 kb, collected from an agarose gel, were methylated with EcoIU methylase and li ...
... acidocaldarius ATCC 27009 chromosomal DNA was constructed as follows. Chromosomal DNA from B. acidocaldarius was isolated according to Marmur (1961). The DNA was digested partially with HaeIII. DNA fragments of 5.0 to 6.5 kb, collected from an agarose gel, were methylated with EcoIU methylase and li ...
A non-canonical pathway for aromatic amino acid biosynthesis in
... Expression and purification of OE1472F, OE2019F & OE1477R ............ 100 Expression of OE1472F in H. salinarum......................................................... 100 ...
... Expression and purification of OE1472F, OE2019F & OE1477R ............ 100 Expression of OE1472F in H. salinarum......................................................... 100 ...
Preneoplastic lesions of the lung
... authors have therefore suggested using size (for example 5 mm) to distinguish between AAH and BAC. As in the squamous cell carcinoma sequence, other problems with the classification of AAH relate to reproducibility and uniformity, and lack of data about progression from hyperplasia to mild, and then ...
... authors have therefore suggested using size (for example 5 mm) to distinguish between AAH and BAC. As in the squamous cell carcinoma sequence, other problems with the classification of AAH relate to reproducibility and uniformity, and lack of data about progression from hyperplasia to mild, and then ...
The Drosophila Gene Disruption Project: Progress
... and have many other applications for analyzing gene function. Here we describe the addition of 7600 new strains, which were selected from .140,000 additional P or piggyBac element integrations and 12,500 newly generated insertions of the Minos transposon. These additions nearly double the size of t ...
... and have many other applications for analyzing gene function. Here we describe the addition of 7600 new strains, which were selected from .140,000 additional P or piggyBac element integrations and 12,500 newly generated insertions of the Minos transposon. These additions nearly double the size of t ...
Biomarkery a mechanismy toxicity
... (effects at lower concentrations than expected from Kow, molecular mechanisms not fully clear) 3) Further, some compounds with reactive properties may directly - and nonspecifically (nonselectively) - react and modify any biological macromolecule (lipids, proteins, nucleic acids) (effects at even lo ...
... (effects at lower concentrations than expected from Kow, molecular mechanisms not fully clear) 3) Further, some compounds with reactive properties may directly - and nonspecifically (nonselectively) - react and modify any biological macromolecule (lipids, proteins, nucleic acids) (effects at even lo ...
Streptococcus pneumoniae Senses a Human-like Sialic Acid
... experiment with TIGR4DnanA and did not detect any nanA transcription in these samples (data not shown). Intracellular Signaling of Sialic Acid Leads to Higher Transcription of the Two-Component System CiaRH in Response to Neu5Ac Compared with Neu5Gc Because strain TIGR4 signals differentially in res ...
... experiment with TIGR4DnanA and did not detect any nanA transcription in these samples (data not shown). Intracellular Signaling of Sialic Acid Leads to Higher Transcription of the Two-Component System CiaRH in Response to Neu5Ac Compared with Neu5Gc Because strain TIGR4 signals differentially in res ...
TbMP42 is a structure-sensitive ribonuclease that likely follows a
... cycle (1–3). An editing cycle starts with the annealing of a pre-edited mRNA to a cognate gRNA molecule. The hybridization is facilitated by matchmaking-type RNA/ RNA annealing factors (4–8) that generate a short intermolecular gRNA/pre-mRNA duplex located proximal to an editing site. The pre-mRNA i ...
... cycle (1–3). An editing cycle starts with the annealing of a pre-edited mRNA to a cognate gRNA molecule. The hybridization is facilitated by matchmaking-type RNA/ RNA annealing factors (4–8) that generate a short intermolecular gRNA/pre-mRNA duplex located proximal to an editing site. The pre-mRNA i ...
Genomic Analysis of Arabidopsis Thaliana
... Arabidopsis DNA in 1984. Most of the genome was found to be composed of singlecopy sequences (50–57%), with repetitive sequences (23–27%) derived primarily from chloroplast DNA. The haploid Arabidopsis nuclear genome was calculated to be 70 Mb. Compared to more recent estimates of 115 Mb for single- ...
... Arabidopsis DNA in 1984. Most of the genome was found to be composed of singlecopy sequences (50–57%), with repetitive sequences (23–27%) derived primarily from chloroplast DNA. The haploid Arabidopsis nuclear genome was calculated to be 70 Mb. Compared to more recent estimates of 115 Mb for single- ...
Fanconi anemia and RAD50 deficiency: genetic and functional
... of this thesis is directed at the molecular, cellular and clinical aspects of two groups of inherited diseases that serve as models for the elucidation of causes and consequences of genetic instability. The genes whose defects cause these diseases are referred to as “caretaker” genes since they appe ...
... of this thesis is directed at the molecular, cellular and clinical aspects of two groups of inherited diseases that serve as models for the elucidation of causes and consequences of genetic instability. The genes whose defects cause these diseases are referred to as “caretaker” genes since they appe ...
Stage-specific expressions of four different ribonuclease H genes in
... have many similarities in metabolic pathways and vital molecular structures with humans, they harbor many unique biochemical features that can be exploited for the development of rational chemotherapy against these human pathogens. The diseases caused by these pests are incurable in the chronic stag ...
... have many similarities in metabolic pathways and vital molecular structures with humans, they harbor many unique biochemical features that can be exploited for the development of rational chemotherapy against these human pathogens. The diseases caused by these pests are incurable in the chronic stag ...
Genetics of Primary ciliary dyskinesia - HAL
... mutations have been identified in a second gene encoding a dynein heavy chain, DNAH5 33 34. DNAH5, located on chromosome 5, codes for a protein orthologous of the Chlamydomonas axonemal heavy chain of the ODA. The human DNAH5 gene, which spans 250 kb, comprises 80 exons. So far, DNAH5 mutations hav ...
... mutations have been identified in a second gene encoding a dynein heavy chain, DNAH5 33 34. DNAH5, located on chromosome 5, codes for a protein orthologous of the Chlamydomonas axonemal heavy chain of the ODA. The human DNAH5 gene, which spans 250 kb, comprises 80 exons. So far, DNAH5 mutations hav ...
Distortion of quantitative genomic and expression
... regarding reproducibility of these techniques have been raised by cross-validation studies in different laboratories (1–5). Strategies to mitigate variability in the results obtained from replicate studies have focused on standardizing technical factors, such as array production, RNA synthesis, labe ...
... regarding reproducibility of these techniques have been raised by cross-validation studies in different laboratories (1–5). Strategies to mitigate variability in the results obtained from replicate studies have focused on standardizing technical factors, such as array production, RNA synthesis, labe ...
Isolation and characterization of a repeated sequence (RPS1) of
... were virtually identical; however, one or two chromosomes were variable in size (Asakura et al., 1991). In virtually all cases, the chromosome that varied in size was chromosome 2. This suggested that chromosome 2 is too variable to be useful for distinguishing between strains. A similar variable ch ...
... were virtually identical; however, one or two chromosomes were variable in size (Asakura et al., 1991). In virtually all cases, the chromosome that varied in size was chromosome 2. This suggested that chromosome 2 is too variable to be useful for distinguishing between strains. A similar variable ch ...
A Pneumocystis carinii multi-gene family with
... 1993). The library was screened with a cDNA clone containing a region of a P. carinii f. sp. carinii MSG gene (EMBL accession number 20870, donated by C. J. Delves and F. Volpe), as part of a study examining subtelomeric sequences in P. carinii. A relatively high number of recombinant plaques gave p ...
... 1993). The library was screened with a cDNA clone containing a region of a P. carinii f. sp. carinii MSG gene (EMBL accession number 20870, donated by C. J. Delves and F. Volpe), as part of a study examining subtelomeric sequences in P. carinii. A relatively high number of recombinant plaques gave p ...
Theoretical questions
... of nucleoproteins can be detected in hydrolysate with reactions, which are specific to each constituent of nucleoproteins. Reagents and materials. 500 mg of fresh yeasts or 100 mg of dried yeasts, 10% solution of sulfuric acid, pipettes, filter paper, water bath. Method. Into large tube (15 x 1.5 cm ...
... of nucleoproteins can be detected in hydrolysate with reactions, which are specific to each constituent of nucleoproteins. Reagents and materials. 500 mg of fresh yeasts or 100 mg of dried yeasts, 10% solution of sulfuric acid, pipettes, filter paper, water bath. Method. Into large tube (15 x 1.5 cm ...
FEMS Microbiology Letters
... plasmid than with the chrA gene alone (Fig. 1a). This increased chromate resistance conferred by the complete operon was more pronounced under low-sulfate growth conditions (0.05 mM sulfate; data not shown). Because the chrAC genes conferred a resistance level similar to that of chrA alone (data not ...
... plasmid than with the chrA gene alone (Fig. 1a). This increased chromate resistance conferred by the complete operon was more pronounced under low-sulfate growth conditions (0.05 mM sulfate; data not shown). Because the chrAC genes conferred a resistance level similar to that of chrA alone (data not ...
Occurrence of phosphate acquisition genes in Prochlorococcus cells
... fragments containing pstS in higher abundance in the Caribbean Sea compared with the Eastern Pacific Ocean (Rusch et al., 2007) (see also Fig. 1C and D for the same trend). Some cyanophages carry phosphate uptake genes including pstS (Sullivan et al., 2005), so we removed any GOS hit if the paired-e ...
... fragments containing pstS in higher abundance in the Caribbean Sea compared with the Eastern Pacific Ocean (Rusch et al., 2007) (see also Fig. 1C and D for the same trend). Some cyanophages carry phosphate uptake genes including pstS (Sullivan et al., 2005), so we removed any GOS hit if the paired-e ...
hindlimb - bthsresearch
... engineered to express a gene of interest • The embryo can then be injected with the virus at an early stage • As virus spreads through the developing tissue, gene expression is turned on • In this way we can express genes ectopically in chick tissue – Like a gain of function mutation in a mouse, but ...
... engineered to express a gene of interest • The embryo can then be injected with the virus at an early stage • As virus spreads through the developing tissue, gene expression is turned on • In this way we can express genes ectopically in chick tissue – Like a gain of function mutation in a mouse, but ...
M261 MHC class I antigen presentation April 17, 2000
... recognizes a complex of antigenic peptide and MHC. One consequence of this is that a T cell specific for peptide x and a particular MHC allele, MHCa (left panel), will not recognize the complex of peptide x with a different MHC allele, MHCb (center panel), or the complex of peptide y with MHCa (righ ...
... recognizes a complex of antigenic peptide and MHC. One consequence of this is that a T cell specific for peptide x and a particular MHC allele, MHCa (left panel), will not recognize the complex of peptide x with a different MHC allele, MHCb (center panel), or the complex of peptide y with MHCa (righ ...
PREDICTION OF THE INTERACTION OF HIV
... immunodeficiency virus type 1 (HIV-1) is integration of the double-stranded retroviral DNA into the genome of the host cell. HIV-1 integrase, the enzyme that inserts the vital DNA into the host chromosome, is an attractive and rational target for anti-AIDS drug design because it is essential for HIV ...
... immunodeficiency virus type 1 (HIV-1) is integration of the double-stranded retroviral DNA into the genome of the host cell. HIV-1 integrase, the enzyme that inserts the vital DNA into the host chromosome, is an attractive and rational target for anti-AIDS drug design because it is essential for HIV ...