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genetics, health and disease
genetics, health and disease

... In order to make use of the information stored in its DNA, a cell needs to express that information - that is, to produce the proteins encoded by the genes. Different types of cells (for example bone, liver and skin cells) contain exactly the same DNA, but they have different functional characterist ...
LP - Columbia University
LP - Columbia University

... 2. Nuclear membrane (compartmental separators) disassembled . Disassembly is temporary -- membrane components not lost, just taken apart into subunits. (Lego castle disassembled -- will be reassembled into two smaller castles after division). 3. Spindle -- have set of fibers attached to chromosomes ...
Biology Spring Final Exam Review
Biology Spring Final Exam Review

... Use and venn diagram to compare and contrast passive transport and active transport. Include examples of each. What is unique about osmosis? Describe the overall purpose and process of photosynthesis. Describe the overall purpose and process of cellular respiration. Label the chloroplast/photosynthe ...
Genome-wide analysis of DNA copy-number
Genome-wide analysis of DNA copy-number

... red/green fluorescence ratios for the autosomal cDNAs (solid line) and X-chromosomal cDNAs (dashed line), plotted as percentage of cDNAs on the ordinate versus red/green fluorescence ratio (binned by intervals of 0.2, upper boundary of bin indicated) on the abscissa. The leftward tailing in the dist ...
Science 102 Lab 3
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... differentiate into different types of cells that will perform specialized roles in the functioning of the whole organism. The information that controls the behavior and differentiation of each cell is carried in the chromosomes. In lab 1, you saw darkstaining nuclei in cells. These nuclei stain dark ...
Linkage, Recombination, and Crossing Over
Linkage, Recombination, and Crossing Over

GENETICS = Scientific study of inheritance
GENETICS = Scientific study of inheritance

... mode of inheritance is ______________________; shown by a capital letter : A Recessive trait = the trait that is hidden in a monohybrid cross where the mode of inheritance is_________________; ______________shown by a lower case letter : a Homozygous = the two alleles for a trait that an organism ha ...
Pathology Test 2 Review
Pathology Test 2 Review

... Oncoproteins—products of oncogenes which resemble the normal products of protooncogenes except that oncoproteins are devoid of important regulatory elements, and their production in the transformed cell does not depend on growth factors or other external signals Promoters—non-carcinogenic chemicals ...
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Molecular Evolutionary Analysis of Classical Swine Fever Virus
Molecular Evolutionary Analysis of Classical Swine Fever Virus

... which consists of 5´- and 3´- untranslated regions (UTR) flanking a single large open reading frame (ORF) coding for a polyprotein of about 3898 amino acids (2). The polyprotein is processed to four structural proteins (C, Erns, E1, E2) and eight non-structural (Npr°, p7, NS2, NS3, NS4A, NS4B, NS5A, ...
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Regulation of gene expression: Eukaryotic

... Termination of Transcription in Prokaryotes • A specific nucleotide sequence acts as a termination signal, about 40 base pairs in length • Sometimes a special protein called termination factor, rho is required for termination • At termination, RNA dissociates from DNA and enzyme (RNA polymerase) fa ...
Editing the Mushroom
Editing the Mushroom

Application of PCR-technique in biological labs
Application of PCR-technique in biological labs

... genes into prokaryotes. Most eukaryotic genes contain introns in the genome but not in the mature mRNA, the cDNA generated from a RT-PCR reaction is the DNA sequence which is directly translated into protein after transcription. When these genes are expressed in prokaryotic cells for protein product ...
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Gene Section IRF1 (interferon regulatory factor 1) Atlas of Genetics and Cytogenetics

... expression in the breast tumour cell line, MCF-7 was studied and found to be induced by IFN-gamma inducible transcription factor IRF1. Further studies have shown that IRF1 behaves as a tumour suppressor gene in breast cancer through caspase activation and induction of apoptosis. This suppression of ...
Fishel, R., Lescoe, M. K., Rao, M. R., Copeland, N. G., Jenkins, N. A.
Fishel, R., Lescoe, M. K., Rao, M. R., Copeland, N. G., Jenkins, N. A.

... protein interacts with mismatched nucleotides but cannot interact with other proteins in the repair pathway, thus interfering with normal mismatch repair. To gain an insight into whether hMSH2 plays a role in mismatch repair, the hMSH2 protein was expressed in E. coli under control of the lac promot ...
Diapositiva 1
Diapositiva 1

... sequence snippets). A typical symptom is that a gene appears to map to multiple loci on the same chromosome, with very high sequence similarity. – But there are also sequences that are nearly indentical, but duplicated. This has happened not long ago in evolution by means of transposable elements. ...
Examples of Topic Proposals
Examples of Topic Proposals

... protein sorting system. To determine which protein sorting pathways are operative and which genes are essential in drug resistance, priority will be given to genes overexpressed in the Rev4 mutant and those that control the operation of the endosomal and endosomeindependent routes to the vacuole. In ...
cs 253: principles of plant breeding
cs 253: principles of plant breeding

... The results of this experiment led Mendel to formulate his second law. Mendel's Second Law - the law of independent assortment; during gamete formation the segregation of the alleles of one allelic pair is independent of the segregation of the alleles of another allelic pair It does inevitably cover ...
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Chapter 16 Cell Cycle Regulation and Cancer

... amplified in the green staining region; single copies are seen as the small green dots; normal chromosomes are stained red. ...
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Evolution

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nuclear structure (2): the nucleolus

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preparation for end of course biology exam
preparation for end of course biology exam

... A purebred recessive male hamster is crossed with a female hamster with an unknown genotype. They have 12 offspring. 6 of the offspring have curly fur and the other 6 have straight fur. a. Illustrate, with a Punnett square, the necessary genotype of the mother hamster to obtain ½ curly-furred and ½ ...
Next-Generation Sequencing Applications Complement
Next-Generation Sequencing Applications Complement

... increasing the amount of lab work, and the total time required to yield meaningful answers. In these cases next-generation sequencing (NGS) complements FISH well. Performing both methods simultaneously could potentially reach desired answers more quickly. ...
Lecture 2: Applications of Tissue Culture to Plant Improvement
Lecture 2: Applications of Tissue Culture to Plant Improvement

... • Trait that is encoded by a single gene • A means of driving expression of the gene in plant cells (Promoters and terminators) • Means of putting the gene into a cell (Vector) • A means of selecting for transformants • Means of getting a whole plant back from the single transformed cell (Regenerati ...
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Vectors in gene therapy

Gene therapy utilizes the delivery of DNA into cells, which can be accomplished by several methods, summarized below. The two major classes of methods are those that use recombinant viruses (sometimes called biological nanoparticles or viral vectors) and those that use naked DNA or DNA complexes (non-viral methods).
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