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Dominant Suppressors of Yeast Actin Mutations That Are Reciprocally Suppressed.
Dominant Suppressors of Yeast Actin Mutations That Are Reciprocally Suppressed.

... pseudorevertants carrying dominant suppressors of the temperature-sensitive (Ts) actl-1 mutation. Of 30 independent revertants analyzed, 29 were found to carry extragenic suppressor mutations and of these, 24/24 tested were found to be linked to each other. This linkage group identifies a new gene S ...
Compound leaves: equal to the sum of their parts?
Compound leaves: equal to the sum of their parts?

... compound leaves, such as pea, tomato, grapevine and poppy, FLO/LFY expression is prolonged during leaf development and accompanies organogenesis at the marginal blastozone (Busch and Gleissberg, 2003). Therefore, it is possible that FLO/LFY also functions in compound leaf development in species othe ...
Document
Document

... A gene is a segment of DNA that tells the cell how to make a particular polypeptide. The location of a gene on a chromosome is called a locus. A gene has the same locus on both chromosomes in a pair of homologous chromosomes. In genetics, scientists often focus on a single gene or set of genes. Geno ...
Ribosomal frameshifting in decoding antizyme mRNAs from yeast
Ribosomal frameshifting in decoding antizyme mRNAs from yeast

... years. As pointed out previously we have been unable to amplify it from human genomic DNA (14). Based on these and other considerations, we now believe that this cDNA is a contaminant, most likely a mammalian antizyme 1 gene belonging to an unidentified rabbit or hare species. In our search for new a ...
The Rad50 Signature Motif: Essential to ATP Binding and
The Rad50 Signature Motif: Essential to ATP Binding and

... Rad50 and the ABC transporter family include the Walker A and Walker B motifs, the Q loop, a histidine residue in the switch region, a D-loop, and a conserved LSGG sequence.22 – 24,10 This conserved sequence, LSGG, is the most specific and characteristic motif of this family and is thus known as the ...
Case Report Section
Case Report Section

... ALL panel DNA probes including CEP 4, 10, and 17 alpha satellite probes, LSI MLL dual-color break apart probe, BCR/ABL and TEL/AML1 dual-fusion translocation probes was performed (Abbott Molecular, ...
Nucleotide Sequence of fruA, the Gene Specifying Enzyme IIfru of
Nucleotide Sequence of fruA, the Gene Specifying Enzyme IIfru of

... Strain HK 1 145 contains a transposon (zeg : :TnlO) integrated in the genome at a site which maps very close to the region specifyingthe enzymes of fructose uptake and phosphorylation (min 46). When tetracycline-sensitive derivatives of such tetracycline-resistant organisms were selected, some of th ...
Synonymous Codon Usage, Accuracy of Translation, and Gene
Synonymous Codon Usage, Accuracy of Translation, and Gene

... these codons (Akashi 1994). In the rest of the text, the terms “accuracy of translation” and “fidelity of protein synthesis” include the three aspects of translation described above. The effect of translational errors on the function of the protein is not the same for all amino acids. Indeed, some ...
manualE6901
manualE6901

... pTXB1 (NEB #N6707) contains a mini-intein from the Mycobacterium xenopi gyrA gene (Mxe GyrA intein; 198 amino acid residues) that has been modified to undergo thiol-induced cleavage at its N-terminus (3,5). The vector allows for the purification of a target protein without any extra amino acids by c ...
- Wiley Online Library
- Wiley Online Library

... process called mitosis. During gametogenesis, two successive rounds of chromosome segregation following only a single round of DNA replication produce cells with only a single set of chromosomes (haploids) from cells initially bearing two copies (diploids). These specialized divisions are called mei ...
Nucleic Acids - Farmasi Unand
Nucleic Acids - Farmasi Unand

... usually inhibit transcription whereas those acting on RNA normally inhibit translation. • In both cases the net result is the prevention or slowing down of cell growth and division. prof. aza ...
Structural organization of the components of the cell wall from
Structural organization of the components of the cell wall from

... The organization of the components of the cell wall from Candida albicans was studied by means of sequential treatment with hot SDS, anhydrous ethylenediamine (EDA) and l y t i c enzymes, followed by chemical and microscopic analyses of the different separated fractions. The EDA-insoluble fraction r ...
Intervention strategies for mitochondrial disease
Intervention strategies for mitochondrial disease

... • Specific targeting of mitochondria by small molecules (referred to as “cargo”) can be achieved by • Protein-based cargo can be coupled to a mitochondrial targeting sequence recognized by the mitochondrial protein import machinery • Coupling the cargo to a delocalized lipophilic cation, leading to ...
Genetic Evidence for a Silent SUC Gene in Yeast.
Genetic Evidence for a Silent SUC Gene in Yeast.

... 1.-Models for the structure of suco alleles. The diagrams show schematic representations (not to scale) of a chromosome carrying a SUC locus; the filled circle represents the centromere. (a) A SUC+ allele at the SUC locus is represented by an open bar. (b) A suc0 allele is depicted as a “silent” gen ...
Hereditary Proteinuria Syndromes and Mechanisms of Proteinuria
Hereditary Proteinuria Syndromes and Mechanisms of Proteinuria

... not have a crucial role, because intravenous glycosaminoglycan-degrading enzymes can affect glycosaminoglycans in all three layers of the filtration barrier. Moreover, genetically engineered mice whose glomerular basement membrane contains heparan sulfate–deficient perlecan or lacks agrin do not hav ...
Natural Selection, Infectious Transfer and the Existence Conditions
Natural Selection, Infectious Transfer and the Existence Conditions

Category 2000
Category 2000

... Peroxisome proliferator-activated receptor coactivator 1 (PPARGC1A) is a versatile coactivator with many functions, but is primarily involved in fat and energy metabolism. Therefore, it is a very interesting candidate gene for meat quality, which is one of the most important criteria in pig selectio ...
Altered Fermentative Metabolism in
Altered Fermentative Metabolism in

... anaerobic induction was performed and compared with immunoblots for PFL1 in the various mutant strains (Figure 3D). Based on this analysis, <2% of the PFL1 protein accumulated in pfl1-2 and pfl1-3 relative to wild-type cells (Figure 3D). Additionally a double mutant was generated by crossing a singl ...
Anatomy of a Cell :
Anatomy of a Cell :

... Cells use DNA (deoxyribonucleic acid) for their genetic material, and all cells contain the transcriptional and translational enzymes to read it and use the information to construct more cell components. However, simply having genetic material does not define life: viruses have genetic material cont ...
Global Transcriptional Profiling Reveals Distinct Functions of Thymic
Global Transcriptional Profiling Reveals Distinct Functions of Thymic

... et al., 2012). Both hematopoietic agerelated dysfunction and degeneration of the thymic stromal compartment likely contribute to thymic involution (BerentMaoz et al., 2012; Chinn et al., 2012). Although reduced levels of the transcription factor Foxn1 contribute to TEC atrophy (Chen et al., 2009) an ...
Reebops: A "Model" Organism for Teaching Genetic Concepts
Reebops: A "Model" Organism for Teaching Genetic Concepts

... both X and Y sex chromosomes, we know it is a male. Human females have two X chromosomes (XX). Now, let’s think about what happens during reproduction. If both the mother’s and father’s cells have 46 chromosomes, the joining of two cells during fertilization should produce 92 chromosomes in each c ...
TOTAL LYMPHOCYTE COUNT AMONG PATIENTS WITH HIV
TOTAL LYMPHOCYTE COUNT AMONG PATIENTS WITH HIV

... heterosexual sex partners of people with AIDS (Friedland, Klein 1987). Studies of these diverse groups led to the conclusion that AIDS is an infectious disease spread by blood, sexual contact and perinatally from mother to child. Two types of HIV infect humans: HIV-1 and HIV-2. Although a variety o ...
Zinc Ions and Cation Diffusion Facilitator Proteins
Zinc Ions and Cation Diffusion Facilitator Proteins

... ectodermal blast cells, P5.p, P6.p, and P7.p. In wildtype hermaphrodites, the anchor cell of the somatic gonad signals to P6.p using a LIN-3 epidermal growth factor-like ligand. LIN-3 binds to the LET-23 RTK, and this leads to the activation of the SEM-5 adaptor protein, the LET-341 RasGEF, LET-60 R ...
Phylogenetic Relationships between the Western Aster Yellows
Phylogenetic Relationships between the Western Aster Yellows

... were used to make phylogenetic comparisons between prokaryotes (10, 57, 58). Comparative analyses of full-length 16s rRNA genes have allowed an even more detailed evaluation of phylogenetic relationships among microorganisms. Evolutionary distances between organisms are calculated on the basis of th ...
A Fitness-Independent Evolvability Measure for Evolutionary
A Fitness-Independent Evolvability Measure for Evolutionary

... are employed. A method for calculating the Euclidean distance between two genomes of different structures and lengths was suggested in [9], [10]. The basic idea is similar to pairwise gene sequence alignment, where the number of matches is maximized and the number of gaps (mismatches) is minimized. ...
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Vectors in gene therapy

Gene therapy utilizes the delivery of DNA into cells, which can be accomplished by several methods, summarized below. The two major classes of methods are those that use recombinant viruses (sometimes called biological nanoparticles or viral vectors) and those that use naked DNA or DNA complexes (non-viral methods).
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