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Text - Enlighten: Publications
Text - Enlighten: Publications

... outcome can arise from differences between either hosts, pathogens, or both. In trypanosome biology, variation in parasite virulence has been well documented but the genetic basis for this has been largely unexplored. Classically, the two T. brucei subspecies have been described as causing different ...
The causes and molecular consequences of polyploidy in
The causes and molecular consequences of polyploidy in

... produced due to pre-meiotic, meiotic, or post-meiotic aberrations, recent studies have shown that ...
Instructions fro BLAST Alignment of sequences
Instructions fro BLAST Alignment of sequences

... Background on BRCA1 For cells to function properly, they need to be able to repair errors in their DNA. These errors can arise when DNA is being copied, or when DNA somehow becomes damaged when exposed to chemicals or radiation. The breast cancer susceptibility gene (BRCA1) encodes a protein that is ...
The molecule that revolutionised and illuminated cell biology started
The molecule that revolutionised and illuminated cell biology started

... emission, but other amino acids also played smaller roles,’ he explains. By gradually making mutations in the gene to carefully change the amino acids, Tsien was able to tweak GFP to create a broad palette of colours and improve overall brightness. The new colours were an important step forward, as ...
Volume 1, Issue 1, November 2012
Volume 1, Issue 1, November 2012

... Department of Physical Sciences and 6Department of Mathematics and Science, Alabama State University, 915 S. Jackson St. Montgomery, Alabama 36101 USA ...
Distinguishing Among Evolutionary Models for the Maintenance of
Distinguishing Among Evolutionary Models for the Maintenance of

... end joining (NHEJ; reviewed in Paques and Haber 1999). The difference in the 2 pathways is largely based on whether homologous sequences are used as a template during double-strand break repair, and this difference can also be used to infer the mechanism by which individual genes are duplicated (une ...
DNA barcoding parasite organisms found in terrestrial
DNA barcoding parasite organisms found in terrestrial

... new areas with their level of overall diversity across a region (Martiny et. al. 2006). As this broad and lofty goal, to discover and map worldwide biodiversity, has been identified by the scientific community, several studies have made efforts to map terrestrial and aquatic biodiversity (e.g. Colwe ...
"Silencing of Gene Expression in Cultured Cells Using Small
"Silencing of Gene Expression in Cultured Cells Using Small

... percent confluence of adherent cells shown in Table 27.1.2, seed the cells in multiple-well plates at a minimum of two different densities 1 day prior to transfection. In this protocol, 75% seeding density is used to plate four 12-well plates to perform a transfection with two siRNAs, an irrelevant ...
Plasma membrane
Plasma membrane

... a. List the general functions of the plasma membrane. b. Which structural component of the plasma membrane is mostly responsible for its ability to isolate a cell from its external environment? c. Which type of integral protein allows water and small ions to pass through the plasma membrane? © 2011 ...
Pearson science 10 Teaching Program 3–4 weeks Chapter 1 DNA
Pearson science 10 Teaching Program 3–4 weeks Chapter 1 DNA

... organisms. They will use models and diagrams to represent relationships between DNA, genes and chromosomes. They will be able to explain the role of meiosis and fertilisation in the passing on of genetic information, describe patterns of inheritance of a simple dominant/recessive characteristic thro ...
The Development of a Genetic Marker for Resistance to Fescue
The Development of a Genetic Marker for Resistance to Fescue

... sequence which may serve as a marker for susceptibility to Fescue Toxicosis. The sequence variation was an intronic A→G single nucleotide polymorphism (SNP) that was found in the region of the DRD2 gene, creating the genotypes AA, AG and GG. In this study, the informativeness of this genetic marker ...
Lectures prepared by Christine L. Case Chapter 8 Microbial Genetics
Lectures prepared by Christine L. Case Chapter 8 Microbial Genetics

Microdeletions on the long arm of the Y chromosome
Microdeletions on the long arm of the Y chromosome

... AZFa region—namely, AZFaT1. The expression of AZFaT1, USP9Y, UTY, and DBY have been examined in four patients with AZFa microdeletions.55 In three of the patients AZFaT1, USP9Y, and DBY were deleted, but UTY was intact; all four patients had the Sertoli cell–only syndrome. The fourth patient, howeve ...
Gene mapping and medical genetics Human chromosome 8
Gene mapping and medical genetics Human chromosome 8

... lipase (LPL) deficiency may result from a primary 8q24 region, where the MYC gene resides.'20 The defect in the LIPD gene itself or from a defect in the major or common rearrangement, t(8;14)(q24;q32), APOC2 gene (on chromosome 19)107 which produces involves a translocation of the MYC locus to the a ...
The Family of SMF Metal Ion Transporters in Yeast Cells*
The Family of SMF Metal Ion Transporters in Yeast Cells*

... Properties of Yeast Null Mutants in the Various SMF Genes—The main feature of the SMF1 null mutant was its sensitivity to EGTA concentrations, toward which the parental wild-type strain was insensitive (8). The growth inhibition of ⌬SMF1 in the presence of EGTA could be alleviated by low concentrati ...
Evolutionary Origin and Adaptive Function of Meiosis
Evolutionary Origin and Adaptive Function of Meiosis

... developmental process. In order for a bacterium to bind, take up and recombine exogenous DNA into its chromosome it must enter a special physiological state, referred to as competence. Development of competence in Bacillus subtilis requires expression of about 40 genes (Solomon and Grossman, 1996). ...
DNA-Catalyzed Covalent Modification of Amino Acid Side Chains in
DNA-Catalyzed Covalent Modification of Amino Acid Side Chains in

Skodova-Euk-Cell-2013
Skodova-Euk-Cell-2013

... threonine, which are abundantly present in the tsetse fly (2). This stage has a fully developed mitochondrion containing a full complement of cytochromes and most of the enzymes of the Krebs cycle (2, 3). Unexpectedly, however, the Krebs cycle does not work as a true cycle such as is seen in other e ...
Supplementary Figure Legends (doc 52K)
Supplementary Figure Legends (doc 52K)

... Supplementary Figure S2. Distribution of the ‘heavy’ and ‘light’ DNA in separated SIP fractions. The top of each panel shows the DGGE profiles of bacterial PCR products from separated (a) [13C]-phenanthrene fractions aligned to equivalent [12C]-phenanthrene fractions, (b) [13C]naphthalene fractions ...
DNA Specificity of the Bicoid Activator Protein Is Determined by
DNA Specificity of the Bicoid Activator Protein Is Determined by

... at position 1 and still activated a Bicoid site target. LexABicoid mutant A5 contained a Lys+Ala substitution at position 5 and also activated a Bicoid site target but at reduced levels. However, activation by this mutant was also reduced using a LexA site target, perhaps because the protein is less ...
CHEMOTHERAPY PHARMACOLOGY
CHEMOTHERAPY PHARMACOLOGY

... Platinum compounds • Mechanism of action: React with nucleophilic sites on DNA causing DNA ...
Microarrays and Cancer - URMC
Microarrays and Cancer - URMC

... 4. Print ONE copy of Appendix B that includes pieces for the microarray class simulation. 5. Make a transparency of the microarray chart from Appendix B. For each class (32 students or less), make one copy of the mRNA strips from normal cells (see Appendix B), cut these into strips, and place strips ...
109 GENES OF ARABIDOPSIS THALIANA INVOLVED IN WAX
109 GENES OF ARABIDOPSIS THALIANA INVOLVED IN WAX

... In animals, such proteases activate precursors of hormones, growth factors, or receptors involved in the control of various developmental processes, including embryonic patterning and proper epidermal differentiation. Although many members of this family of proteases were reported in plants [29; 26] ...
Ammonium transport in Escherichia coli: localization and nucleotide
Ammonium transport in Escherichia coli: localization and nucleotide

... (20 mM) as sole source of nitrogen, as described previously (Jayakumar et al., 1986). In complementation assays, restoration of the Amtphenotype to A52653 was monitored on M9 plates containing 100 p ~ ammonium acetate as sole nitrogen source, as previously described (Jayakumar at al., 1989). Strain ...
GATA factor function in heart development
GATA factor function in heart development

... pnr gene affects heart development. (A-D) Expression of pnr results in overexpression of a D-mef2 heart enhancer and cardiac hyperplasia. Stage 11 (A,B) and 16 (C,D) embryos stained for lacZ expression under the control of a D-mef2 cardial cell enhancer are shown. (A,C) WT, wild-type embryos; (B,D) ...
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Vectors in gene therapy

Gene therapy utilizes the delivery of DNA into cells, which can be accomplished by several methods, summarized below. The two major classes of methods are those that use recombinant viruses (sometimes called biological nanoparticles or viral vectors) and those that use naked DNA or DNA complexes (non-viral methods).
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