Applied and Environmental Microbiologyy
... For flow-chamber experiments, the strains were tagged with the green fluorescent protein (GFP). This was accomplished by the insertion of a miniTn7PA1/04/03-gfp-T0T1 transposon cassette into the chromosomes of target strains using the suicide construct pBK-miniTn7-gfp3 (25). Plasmid pBK-miniTn7-gfp3 ...
... For flow-chamber experiments, the strains were tagged with the green fluorescent protein (GFP). This was accomplished by the insertion of a miniTn7PA1/04/03-gfp-T0T1 transposon cassette into the chromosomes of target strains using the suicide construct pBK-miniTn7-gfp3 (25). Plasmid pBK-miniTn7-gfp3 ...
NUCLEOTIDES AND NUCLEIC ACIDS
... both DNA and RNA are hydrophilic. The hydroxyl groups of the sugar residues form hydrogen bonds with water. The phosphate groups, with a pKa near 0, are completely ionized and negatively charged at pH 7, and the negative charges are generally neutralized by ionic interactions with positive charges o ...
... both DNA and RNA are hydrophilic. The hydroxyl groups of the sugar residues form hydrogen bonds with water. The phosphate groups, with a pKa near 0, are completely ionized and negatively charged at pH 7, and the negative charges are generally neutralized by ionic interactions with positive charges o ...
Author`s personal copy
... If a highly efficient method for mutant construction is developed, a genome-wide analysis of gene function is possible (Hammelmann and Soppa, 2008). It would not only accelerate the genome-wide identification of the genes responsible for PHA biosynthesis, but may also be used to genetically engineer ...
... If a highly efficient method for mutant construction is developed, a genome-wide analysis of gene function is possible (Hammelmann and Soppa, 2008). It would not only accelerate the genome-wide identification of the genes responsible for PHA biosynthesis, but may also be used to genetically engineer ...
brat regulates cell growth and rRNA synthesis
... third instar brat mutant larvae, although appearing normal in situ, are able to metastasize and form secondary tumors when injected into the abdomen of a wild-type host fly (Woodhouse et al., 1998), thus indicating that brat also has a function in the imaginal discs. Superficially, brat and ncl-1 mu ...
... third instar brat mutant larvae, although appearing normal in situ, are able to metastasize and form secondary tumors when injected into the abdomen of a wild-type host fly (Woodhouse et al., 1998), thus indicating that brat also has a function in the imaginal discs. Superficially, brat and ncl-1 mu ...
PDF
... Cloning and sequences analysis Differentially expressed cDNA was cloned in the pCR4-TOPO vector (Invitrogen, USA) and sequenced. Sequence analysis identified a cDNA fragment of 102 nucleotides. Similarity searches performed using the FASTA algorithm (http://www.ebi.ac.uk/ Tools/fasta/) showed a rele ...
... Cloning and sequences analysis Differentially expressed cDNA was cloned in the pCR4-TOPO vector (Invitrogen, USA) and sequenced. Sequence analysis identified a cDNA fragment of 102 nucleotides. Similarity searches performed using the FASTA algorithm (http://www.ebi.ac.uk/ Tools/fasta/) showed a rele ...
Reactive Oxygen Species and Cellular Defense System
... hydrogen atom from another molecule, bind to another molecule, or interact in various ways with other free radicals. Free radicals can be defined as reactive chemical species having a single unpaired electron in an outer orbit and are continuously produced by the organism’s normal use of oxygen [2]. ...
... hydrogen atom from another molecule, bind to another molecule, or interact in various ways with other free radicals. Free radicals can be defined as reactive chemical species having a single unpaired electron in an outer orbit and are continuously produced by the organism’s normal use of oxygen [2]. ...
Nucleotide sequence of the thioredoxin gene from
... box and a -35 region (Fig. 3); both showing four out of six residues i d e n t i c a l to the respective consensus sequences (30) in E. c o l t . Therefore, it seems as if the transcription of E. c o l t thioredoxin is governed by its own promotor region, which is consistent with the fact that thior ...
... box and a -35 region (Fig. 3); both showing four out of six residues i d e n t i c a l to the respective consensus sequences (30) in E. c o l t . Therefore, it seems as if the transcription of E. c o l t thioredoxin is governed by its own promotor region, which is consistent with the fact that thior ...
Genetics - Max Appeal!
... The extent to which a person is affected is extremely variable and almost impossible to predict. There are almost 200 anomalies caused by the deletion, and each individual could be affected by many (but not all!), just a few issues or have some minor problems, say, not being very good at maths at sc ...
... The extent to which a person is affected is extremely variable and almost impossible to predict. There are almost 200 anomalies caused by the deletion, and each individual could be affected by many (but not all!), just a few issues or have some minor problems, say, not being very good at maths at sc ...
RASPBERRY3 Gene Encodes a Novel Protein Important for Embryo
... protein may be localized in the chloroplast. Complementation analysis supports the possibility that the RASPBERRY3 protein may be involved in chloroplast development. Our experiments demonstrate the important role of the chloroplast, directly or indirectly, in embryo morphogenesis and development. ...
... protein may be localized in the chloroplast. Complementation analysis supports the possibility that the RASPBERRY3 protein may be involved in chloroplast development. Our experiments demonstrate the important role of the chloroplast, directly or indirectly, in embryo morphogenesis and development. ...
Full-Text PDF
... stress. These genes can be classified into two groups: the first group includes genes related to cell metabolism and stress tolerance, and the second group consists of regulatory genes that encode protein kinases or phosphatases and transcription factors [2]. Transcription factors are DNA-binding pr ...
... stress. These genes can be classified into two groups: the first group includes genes related to cell metabolism and stress tolerance, and the second group consists of regulatory genes that encode protein kinases or phosphatases and transcription factors [2]. Transcription factors are DNA-binding pr ...
Similarities between putative transport proteins of plant viruses
... 1981), and of CMV RNA3 strain Q (Davies & Symons, 1988). The sequence of proteins encoded by CMV strain O (Hayakawa et al., 1989) and the very similar strain Y (Nitta et al., 1988) were not separately analysed. Three ORF I product caulimoviral sequences were used: CaMV, Cabb S isolate (Franck et al. ...
... 1981), and of CMV RNA3 strain Q (Davies & Symons, 1988). The sequence of proteins encoded by CMV strain O (Hayakawa et al., 1989) and the very similar strain Y (Nitta et al., 1988) were not separately analysed. Three ORF I product caulimoviral sequences were used: CaMV, Cabb S isolate (Franck et al. ...
Genetics - Max Appeal!
... The extent to which a person is affected is extremely variable and almost impossible to predict. There are almost 200 anomalies caused by the deletion, and each individual could be affected by many (but not all!) or just a few or have some minor problems, say, not being very good at maths at school. ...
... The extent to which a person is affected is extremely variable and almost impossible to predict. There are almost 200 anomalies caused by the deletion, and each individual could be affected by many (but not all!) or just a few or have some minor problems, say, not being very good at maths at school. ...
The role of regulatory variation in complex traits and
... blood had a local eQTL in a recent survey of nearly 1,000 individuals47. In yeast, ~25% of genes had a local eQTL in a comparison of two different isolates41, and many more local regulatory variants are expected to exist in additional yeast strains that have not been studied so far. Indeed, a popula ...
... blood had a local eQTL in a recent survey of nearly 1,000 individuals47. In yeast, ~25% of genes had a local eQTL in a comparison of two different isolates41, and many more local regulatory variants are expected to exist in additional yeast strains that have not been studied so far. Indeed, a popula ...
Transcription in Eukaryotes Eukaryotes have 3 different RNA
... First concensus sequence from lining up several eukaryotic promoters: TATA box ...
... First concensus sequence from lining up several eukaryotic promoters: TATA box ...
03-Biological 42-3-Rosa
... obtained within cytotype A sample, which weakens the hypothesis that they originated through hybridization between the two cytotypes. Therefore, the different cell lines appear to be a consequence of chromosomal alterations (non-disjunction) in the early mitotic divisions of zygotes belonging to the ...
... obtained within cytotype A sample, which weakens the hypothesis that they originated through hybridization between the two cytotypes. Therefore, the different cell lines appear to be a consequence of chromosomal alterations (non-disjunction) in the early mitotic divisions of zygotes belonging to the ...
Cancer - Stanford University
... • B cells are white blood cells that produce antibodies. • A healthy immune system has millions of B cell subpopulations, each expressing a unique antibody. • In myeloma (cancer of mature B cells), all the myeloma cells express the same antibody. • Conclusion: Tumors are monoclonal ...
... • B cells are white blood cells that produce antibodies. • A healthy immune system has millions of B cell subpopulations, each expressing a unique antibody. • In myeloma (cancer of mature B cells), all the myeloma cells express the same antibody. • Conclusion: Tumors are monoclonal ...
Pisum Genetics Volume 26 1994 Preface 1 PGA "Pisum Genetics
... map update provided by the Linkage Committee appears on the cover of Volume 25 and an RFLP map by Ellis et al appears inVolume 25:5. While some areas of the map are beginning to stabilise, there are still some inconsistencies and doubts. Adherence to the proposed Mapping Guidelines (Volume 25:13-14) ...
... map update provided by the Linkage Committee appears on the cover of Volume 25 and an RFLP map by Ellis et al appears inVolume 25:5. While some areas of the map are beginning to stabilise, there are still some inconsistencies and doubts. Adherence to the proposed Mapping Guidelines (Volume 25:13-14) ...
Bioimage_Informatics_2
... cancers in humans, and it turns out that each of those genes has many sites at which mutations can lead to altered gene function. Therefore, conducting an oncogene screen requires assessing changes in cell phenotype for at least 140 genes, each with potentially dozens of different mutations, and in ...
... cancers in humans, and it turns out that each of those genes has many sites at which mutations can lead to altered gene function. Therefore, conducting an oncogene screen requires assessing changes in cell phenotype for at least 140 genes, each with potentially dozens of different mutations, and in ...
The Caenorhabditis elegans NK-2 class
... Fig. 1. The myo-2 enhancer contains multiple elements that cooperate to activate transcription. (A) To characterize the myo-2 enhancer, DNA fragments are cloned upstream of a myo-3::lacZ fusion, which is normally expressed only in body wall muscle. The resulting plasmids are injected into the germli ...
... Fig. 1. The myo-2 enhancer contains multiple elements that cooperate to activate transcription. (A) To characterize the myo-2 enhancer, DNA fragments are cloned upstream of a myo-3::lacZ fusion, which is normally expressed only in body wall muscle. The resulting plasmids are injected into the germli ...
CHAPTER 13 MEIOSIS AND SEXUAL LIFE CYCLES
... Any sexually reproducing species has a characteristic haploid and diploid number of chromosomes. ...
... Any sexually reproducing species has a characteristic haploid and diploid number of chromosomes. ...
Managing Blackleg Resistance Breakdown and Trade Barriers through Blackleg
... • Getting tighter • Increase risk for disease development ...
... • Getting tighter • Increase risk for disease development ...
Anther Development
... identity genes and those involved in stamen primordia specification. Second, and most important, it is not known what target, or downstream, genes are activated transcriptionally by the DEF (AP3), GLO (PI), and PLE (AG) proteins within the stamen primordia. That is, what are the genes whose products ...
... identity genes and those involved in stamen primordia specification. Second, and most important, it is not known what target, or downstream, genes are activated transcriptionally by the DEF (AP3), GLO (PI), and PLE (AG) proteins within the stamen primordia. That is, what are the genes whose products ...